Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   CNO08_RS19035 Genome accession   NZ_CP023465
Coordinates   4761267..4761677 (+) Length   136 a.a.
NCBI ID   WP_096415524.1    Uniprot ID   -
Organism   Lysobacter capsici strain KNU-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4761267..4771379 4761267..4761677 within 0


Gene organization within MGE regions


Location: 4761267..4771379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNO08_RS19035 (CNO08_19035) pilA2 4761267..4761677 (+) 411 WP_096415524.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CNO08_RS19040 (CNO08_19040) - 4761806..4763698 (+) 1893 WP_157754139.1 hypothetical protein -
  CNO08_RS19045 (CNO08_19045) - 4763695..4765707 (+) 2013 WP_157754140.1 hypothetical protein -
  CNO08_RS19050 (CNO08_19050) - 4765926..4766900 (+) 975 WP_157754141.1 hypothetical protein -
  CNO08_RS19055 (CNO08_19055) - 4766887..4767636 (+) 750 WP_096415536.1 class I SAM-dependent methyltransferase -
  CNO08_RS19060 (CNO08_19060) - 4767633..4768694 (+) 1062 WP_096415539.1 NAD(P)-binding domain-containing protein -
  CNO08_RS19065 (CNO08_19065) - 4768674..4769528 (-) 855 WP_157754142.1 GNAT family N-acetyltransferase -
  CNO08_RS19070 (CNO08_19070) - 4769521..4770678 (-) 1158 WP_096415545.1 glycosyltransferase -
  CNO08_RS19075 (CNO08_19075) - 4770675..4771379 (-) 705 WP_051547075.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14307.52 Da        Isoelectric Point: 7.5890

>NTDB_id=247243 CNO08_RS19035 WP_096415524.1 4761267..4761677(+) (pilA2) [Lysobacter capsici strain KNU-14]
MKRQRGFTLIELMIVIAIIGILISIALPAYLDYVARAKNMECLNVAAGAKLSVSETAQDRGSLSLVTATNTGYSFSASSY
CASIDIGASGVITATTSTVNAPVTFTFRPRSIPGGLEWDCSVPNGTNLTLVPAECR

Nucleotide


Download         Length: 411 bp        

>NTDB_id=247243 CNO08_RS19035 WP_096415524.1 4761267..4761677(+) (pilA2) [Lysobacter capsici strain KNU-14]
ATGAAACGGCAACGTGGATTCACTCTGATCGAATTGATGATCGTGATAGCGATCATCGGAATACTGATTTCAATAGCGCT
CCCGGCTTACCTGGACTATGTCGCACGGGCCAAGAACATGGAGTGCCTCAATGTCGCCGCCGGAGCCAAGCTGTCGGTAT
CGGAAACCGCGCAGGACCGCGGCTCGCTGAGCCTGGTCACCGCGACCAACACCGGTTATAGCTTCAGCGCCTCGAGCTAT
TGCGCGAGCATCGATATCGGCGCCAGCGGTGTGATTACCGCGACCACATCCACGGTAAACGCGCCCGTCACTTTCACCTT
CCGCCCCCGCTCCATACCTGGCGGACTGGAATGGGATTGCAGCGTTCCGAATGGAACCAACCTGACCTTAGTGCCTGCCG
AATGCCGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

49.635

100

0.5
  pilA2 Legionella pneumophila strain ERS1305867

48.905

100

0.493
  pilA Ralstonia pseudosolanacearum GMI1000

37.576

100

0.456
  comP Acinetobacter baylyi ADP1

40.541

100

0.441
  pilA/pilA1 Eikenella corrodens VA1

37.5

100

0.419
  pilE Neisseria gonorrhoeae strain FA1090

33.544

100

0.39
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.945

93.382

0.382
  pilE Neisseria gonorrhoeae MS11

38.168

96.324

0.368

Multiple sequence alignment