Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CN548_RS14565 Genome accession   NZ_CP023464
Coordinates   3211721..3212947 (+) Length   408 a.a.
NCBI ID   WP_096042341.1    Uniprot ID   -
Organism   Pseudoalteromonas atlantica strain ECSMB14104     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3206721..3217947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN548_RS14535 ampE 3207179..3208030 (-) 852 WP_096740202.1 beta-lactamase regulator AmpE -
  CN548_RS14540 ampD 3208044..3208598 (-) 555 WP_096042336.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CN548_RS14545 nadC 3208814..3209665 (+) 852 WP_096042337.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CN548_RS14550 - 3210135..3210560 (+) 426 WP_096042338.1 pilin -
  CN548_RS16910 - 3210609..3211004 (+) 396 WP_415907446.1 pilin -
  CN548_RS14560 - 3210985..3211596 (-) 612 WP_096042340.1 fimbrial assembly protein -
  CN548_RS14565 pilC 3211721..3212947 (+) 1227 WP_096042341.1 type II secretion system F family protein Machinery gene
  CN548_RS14570 pilD 3213095..3213985 (+) 891 WP_096042342.1 prepilin peptidase Machinery gene
  CN548_RS14575 coaE 3213987..3214607 (+) 621 WP_096042343.1 dephospho-CoA kinase -
  CN548_RS14580 pilB 3214754..3216430 (+) 1677 WP_096042344.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CN548_RS14585 yacG 3216478..3216708 (+) 231 WP_024031401.1 DNA gyrase inhibitor YacG -
  CN548_RS14590 - 3216776..3217267 (-) 492 WP_096042345.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44432.44 Da        Isoelectric Point: 9.7315

>NTDB_id=247222 CN548_RS14565 WP_096042341.1 3211721..3212947(+) (pilC) [Pseudoalteromonas atlantica strain ECSMB14104]
MAKQQEKKGQDTFVWVGVSARGKRLEGELNGNSIALVKAQLRKQGITPSKVKRKAKPLFGTRLQKITPKDIAMVTRQIAT
MLLAGVPLIQAIEMIGTGATNKSVGKLMETIGDEVKSGQPLSQALRKHPRYFDDLYCDLVASGEQSGALDKIFDRVALYK
EKSEALKSKIKKAMFYPIAVLVVALIVTSILLIFVVPQFQDIFNGFGAELPAFTLFVIAISEFMQEYWWIIVLLIAAFGY
GYKEALLKSQKLRDATDRLILKMPVVGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILDIKAEVS
SGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYLP
IFQLGSVI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=247222 CN548_RS14565 WP_096042341.1 3211721..3212947(+) (pilC) [Pseudoalteromonas atlantica strain ECSMB14104]
ATGGCTAAACAACAAGAAAAAAAAGGGCAAGATACCTTTGTTTGGGTCGGTGTTAGTGCTAGAGGTAAACGACTTGAAGG
TGAGTTAAATGGCAATAGTATAGCTTTAGTTAAAGCCCAGCTTCGTAAACAAGGTATAACCCCATCTAAGGTGAAAAGAA
AAGCAAAACCTCTATTCGGAACTCGTTTACAAAAAATTACACCAAAAGACATCGCAATGGTAACTCGCCAAATAGCGACT
ATGTTATTAGCTGGTGTGCCGTTAATTCAAGCTATAGAGATGATTGGTACTGGCGCCACAAATAAAAGCGTAGGCAAGTT
AATGGAGACTATTGGCGATGAAGTTAAATCAGGCCAACCTCTATCTCAAGCACTCAGAAAACATCCTCGCTACTTTGATG
ATTTATATTGTGACTTAGTCGCATCGGGTGAGCAGTCGGGTGCGCTTGATAAAATATTTGACCGTGTTGCTTTATATAAA
GAAAAGTCAGAAGCGCTTAAGTCAAAAATTAAAAAAGCCATGTTTTACCCTATCGCGGTTTTAGTTGTTGCGCTTATCGT
TACTTCAATACTGCTTATATTTGTAGTACCGCAGTTTCAAGATATTTTTAATGGCTTTGGCGCAGAGCTGCCTGCCTTTA
CCTTATTTGTAATCGCCATATCTGAATTTATGCAAGAGTATTGGTGGATTATTGTTTTACTTATTGCTGCTTTTGGTTAT
GGCTATAAAGAAGCGCTACTAAAAAGCCAGAAATTAAGAGATGCAACAGATAGGCTTATTTTAAAAATGCCTGTTGTCGG
CATGATTTTAAATAAAGCCGCTGTTGCACGATATGCTCGTACGCTTTCGACCACCTTTGCTGCGGGTGTACCCCTTGTTG
ATGCACTGGACTCAGCTGCTGGTGCGTCCGGTAATGCTGTGTATCGCTATGCTATTTTAGATATTAAAGCAGAGGTGAGC
TCGGGTAATCAAATGAATTGGGCGATGCGTAACTCTAAAATATTCCCCGATATGGTTATTCAGATGGTGGCCATTGGTGA
AGAGTCTGGCTCGTTAGATGGTATGCTAGCCAAAGTAGCAACAATTTACGAACAAGAAGTTGATGATGCAGTTGATGGCT
TATCTAGCTTATTAGAGCCGCTTATTATGGCTGTGCTGGGTGTGCTCGTCGGTGGCTTAATTGTTGCCATGTACTTGCCG
ATTTTCCAATTAGGCTCTGTAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.869

97.059

0.6
  pilC Acinetobacter baylyi ADP1

56.423

97.304

0.549
  pilC Acinetobacter baumannii D1279779

55.062

99.265

0.547
  pilC Legionella pneumophila strain ERS1305867

55.416

97.304

0.539
  pilC Vibrio cholerae strain A1552

44.836

97.304

0.436
  pilG Neisseria gonorrhoeae MS11

42.469

99.265

0.422
  pilG Neisseria meningitidis 44/76-A

42.469

99.265

0.422
  pilC Vibrio campbellii strain DS40M4

42.065

97.304

0.409
  pilC Thermus thermophilus HB27

37.659

96.324

0.363

Multiple sequence alignment