Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CN548_RS14580 Genome accession   NZ_CP023464
Coordinates   3214754..3216430 (+) Length   558 a.a.
NCBI ID   WP_096042344.1    Uniprot ID   -
Organism   Pseudoalteromonas atlantica strain ECSMB14104     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3209754..3221430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN548_RS14550 - 3210135..3210560 (+) 426 WP_096042338.1 pilin -
  CN548_RS16910 - 3210609..3211004 (+) 396 WP_415907446.1 pilin -
  CN548_RS14560 - 3210985..3211596 (-) 612 WP_096042340.1 fimbrial assembly protein -
  CN548_RS14565 pilC 3211721..3212947 (+) 1227 WP_096042341.1 type II secretion system F family protein Machinery gene
  CN548_RS14570 pilD 3213095..3213985 (+) 891 WP_096042342.1 prepilin peptidase Machinery gene
  CN548_RS14575 coaE 3213987..3214607 (+) 621 WP_096042343.1 dephospho-CoA kinase -
  CN548_RS14580 pilB 3214754..3216430 (+) 1677 WP_096042344.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CN548_RS14585 yacG 3216478..3216708 (+) 231 WP_024031401.1 DNA gyrase inhibitor YacG -
  CN548_RS14590 - 3216776..3217267 (-) 492 WP_096042345.1 Spy/CpxP family protein refolding chaperone -
  CN548_RS14595 - 3217443..3218132 (+) 690 WP_096042346.1 response regulator -
  CN548_RS14600 - 3218110..3219525 (+) 1416 WP_096740203.1 ATP-binding protein -
  CN548_RS14605 - 3219528..3220475 (+) 948 WP_096740204.1 alpha/beta fold hydrolase -
  CN548_RS14610 - 3220435..3221283 (-) 849 WP_024031405.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61991.17 Da        Isoelectric Point: 5.0828

>NTDB_id=247224 CN548_RS14580 WP_096042344.1 3214754..3216430(+) (pilB) [Pseudoalteromonas atlantica strain ECSMB14104]
MNINSPLLRKFITLGRIDADTVKAKQQEFASTAELISKCGKIDSNDLAEQCIDLFRVPYFDLKDFDPAQIPEDLVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDNKIEQLLDAAGSLDLSADEFKEFADLDTEG
PKETTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVANPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEEDQKKLYMDALGQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINPKAEMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPETLPAEELARQGFSTTQIEDMTL
YAPKGCENCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLQAGFNNLRLSGLRKAADGLTSLAEINRVTNM

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=247224 CN548_RS14580 WP_096042344.1 3214754..3216430(+) (pilB) [Pseudoalteromonas atlantica strain ECSMB14104]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATAACGCTTGGCCGCATTGATGCCGATACCGTAAAAGCAAAACAGCA
AGAGTTTGCCTCAACCGCTGAGCTTATATCTAAATGTGGAAAAATTGACAGCAATGACTTAGCTGAACAATGTATAGACT
TATTTCGCGTACCGTATTTTGATTTAAAAGACTTCGACCCTGCACAAATCCCCGAAGACTTAGTTAAAGAAAAACTAATA
CGTAAACACCATATTTTACCGCTGGTTCAAAAAGGCAGAAAAGTATACATTGCGGCCTCAGATCCGACTGACTACGGTGC
CTTTGAAAACTTTGAATTTAGCACCGGCTTATCGTGCGAAATAGTCGTGGTTGACTATATTCAGCTAGATAACAAAATTG
AGCAACTACTTGATGCTGCAGGCAGCTTAGATTTAAGCGCCGATGAATTTAAAGAGTTTGCCGACTTAGATACTGAAGGC
CCTAAAGAAACCACACCTAAAGATGATGATAAAGACGATGCGCCAATCATTGTTTATATCAATAAAATATTAATGGATGC
CATTAAAAAAGGCGCTTCTGATTTACACTTTGAACCTTACGAGCACAAATACCGTATTCGCTTTCGTATTGATGGTATTT
TGCATGAAGTTGCCAACCCACCCAATACCTTAGCAAGTAAACTGTCGGCGCGTATCAAAGTAATGTCGAGGCTCGATATT
GCTGAAAAACGTAAACCACAAGATGGCCGTATTAAATTAAAAATTACAGAGCGTAAAAGCATCGATTTTCGTGTAAGTAC
TATGCCTACGTTATGGGGCGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCCATGCTAGGTATTGACGTATTAG
GTTACGAAGAGGATCAAAAAAAGCTCTACATGGATGCGCTTGGGCAACCACAAGGCATGATACTAGTTACCGGCCCAACG
GGCTCTGGTAAAACCGTATCGCTTTATACCGGTTTAAATATACTTAACCAGCCAGAGCGTAATATTAGTACCGCCGAGGA
TCCGGTTGAGATAAACCTTGAAGGGGTTAACCAAGTACAAATTAACCCTAAAGCCGAAATGACCTTTGCTAACGCCTTGC
GTGCATTTTTACGTCAAGATCCAGATGTAGTGATGGTAGGTGAAATACGTGACCTAGAAACCGCAGAAATTTCAATAAAA
GCGGCGCAAACAGGTCACTTAGTGTTATCAACACTACATACCAACTCAGCGCCCGAAACCATAACGCGTTTATTAAATAT
GGGCGTGCCTGCGTATAACGTGGCAAGCTCTATAAGTTTGATTATTGCCCAGCGCTTAGCTAGGCGTTTATGCCCTAAGT
GTAAAACCCCAGAAACACTGCCAGCAGAAGAGTTGGCTCGTCAAGGTTTTAGCACAACACAAATTGAAGACATGACACTA
TACGCACCCAAAGGCTGCGAAAATTGCACTGATGGTTATAAAGGCCGTGTGGGTATTTACGAAGTAATGCAAATTACCCC
TGAAATTGCACAAATTATTATGCGCGGTGGTAATTCGCTCGAAATCGCAGAGGTATCGCTACAAGCTGGGTTTAATAACT
TGCGATTATCGGGTTTACGAAAAGCCGCCGATGGTTTAACGTCTCTGGCTGAAATTAATCGCGTAACCAATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.81

100

0.568
  pilB Acinetobacter baylyi ADP1

58.546

91.219

0.534
  pilB Legionella pneumophila strain ERS1305867

53.165

99.104

0.527
  pilB Vibrio cholerae strain A1552

52.799

96.057

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

52.82

95.341

0.504
  pilB Vibrio campbellii strain DS40M4

51.88

95.341

0.495
  pilF Neisseria gonorrhoeae MS11

49.042

93.548

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.645

90.86

0.36

Multiple sequence alignment