Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CLI14_RS11415 Genome accession   NZ_CP023208
Coordinates   2498299..2498724 (+) Length   141 a.a.
NCBI ID   WP_040122945.1    Uniprot ID   A0AAW4AAV7
Organism   Vibrio anguillarum strain ATCC-68554     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2493299..2503724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI14_RS11390 (CLI14_11390) - 2494089..2494862 (-) 774 WP_013857678.1 thioredoxin fold domain-containing protein -
  CLI14_RS11395 (CLI14_11395) xerD 2494876..2495784 (-) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  CLI14_RS11400 (CLI14_11400) fldB 2495937..2496455 (+) 519 WP_013857680.1 flavodoxin FldB -
  CLI14_RS11405 (CLI14_11405) ampD 2496477..2497028 (-) 552 WP_202599069.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CLI14_RS11410 (CLI14_11410) nadC 2497153..2498040 (+) 888 WP_013857682.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CLI14_RS11415 (CLI14_11415) pilA 2498299..2498724 (+) 426 WP_040122945.1 pilin Machinery gene
  CLI14_RS11420 (CLI14_11420) pilB 2498734..2500421 (+) 1688 Protein_2198 type IV-A pilus assembly ATPase PilB -
  CLI14_RS11425 (CLI14_11425) pilC 2500464..2501690 (+) 1227 WP_013857686.1 type II secretion system F family protein Machinery gene
  CLI14_RS11430 (CLI14_11430) pilD 2501729..2502610 (+) 882 WP_013857687.1 A24 family peptidase Machinery gene
  CLI14_RS11435 (CLI14_11435) coaE 2502601..2503209 (+) 609 WP_013857688.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14584.80 Da        Isoelectric Point: 9.4689

>NTDB_id=245322 CLI14_RS11415 WP_040122945.1 2498299..2498724(+) (pilA) [Vibrio anguillarum strain ATCC-68554]
MNTNKKTKQSGFTLIELMIVVAIVGVLAAIAVPQYQKYVAKSEAAAALASITGHRTNVETYVLTQGSFPSTSAALAIPTS
PLGIITYDNASSAAGDILFTFNGTGVSPDMVSKKVAVKRNASGAWSCSTDVALELKPKGCS

Nucleotide


Download         Length: 426 bp        

>NTDB_id=245322 CLI14_RS11415 WP_040122945.1 2498299..2498724(+) (pilA) [Vibrio anguillarum strain ATCC-68554]
ATGAACACAAATAAGAAAACGAAACAAAGTGGTTTTACCTTAATTGAATTGATGATTGTAGTAGCTATAGTCGGTGTTCT
AGCCGCAATTGCAGTTCCGCAATATCAAAAATATGTGGCAAAAAGTGAGGCTGCAGCAGCTTTAGCTTCAATTACGGGCC
ACAGAACAAACGTCGAAACATACGTGCTAACCCAAGGTAGCTTCCCTAGCACATCAGCAGCTCTTGCTATTCCAACATCC
CCTCTTGGTATCATAACTTATGATAATGCCTCATCGGCAGCAGGGGACATTTTATTCACCTTCAATGGCACAGGTGTTAG
CCCAGACATGGTAAGTAAAAAAGTTGCTGTGAAAAGAAATGCTAGTGGTGCATGGAGTTGCAGTACTGATGTGGCATTAG
AGTTAAAACCAAAAGGTTGTAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

48.344

100

0.518
  pilA Vibrio cholerae C6706

48.344

100

0.518
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.344

100

0.518
  pilA Pseudomonas aeruginosa PAK

41.096

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.429

99.291

0.411
  pilA Acinetobacter baumannii strain A118

40.58

97.872

0.397
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.589

100

0.376
  pilA Vibrio parahaemolyticus RIMD 2210633

37.063

100

0.376
  pilA Acinetobacter nosocomialis M2

35.664

100

0.362

Multiple sequence alignment