Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   Kalk_RS17525 Genome accession   NZ_CP022684
Coordinates   4085567..4086823 (-) Length   418 a.a.
NCBI ID   WP_101895483.1    Uniprot ID   A0A2K9LP44
Organism   Ketobacter alkanivorans strain GI5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4080567..4091823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Kalk_RS17495 (Kalk_17470) argJ 4081149..4082381 (+) 1233 WP_101895477.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  Kalk_RS17500 (Kalk_17475) - 4082385..4083347 (+) 963 WP_101895478.1 Nudix family hydrolase -
  Kalk_RS17505 (Kalk_17480) - 4083378..4083710 (+) 333 WP_101895479.1 YegP family protein -
  Kalk_RS17510 (Kalk_17485) yacG 4083779..4083967 (-) 189 WP_101895480.1 DNA gyrase inhibitor YacG -
  Kalk_RS17515 (Kalk_17490) coaE 4083964..4084563 (-) 600 WP_101895481.1 dephospho-CoA kinase -
  Kalk_RS17520 (Kalk_17495) pilD 4084642..4085550 (-) 909 WP_101895482.1 A24 family peptidase Machinery gene
  Kalk_RS17525 (Kalk_17500) pilC 4085567..4086823 (-) 1257 WP_101895483.1 type II secretion system F family protein Machinery gene
  Kalk_RS17530 (Kalk_17505) pilB 4086839..4088536 (-) 1698 WP_101895484.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Kalk_RS17535 (Kalk_17510) - 4088904..4089383 (+) 480 WP_101895485.1 pilin -
  Kalk_RS17540 (Kalk_17515) - 4089494..4089967 (+) 474 WP_101896382.1 pilin -
  Kalk_RS17545 (Kalk_17520) nadC 4089989..4090825 (-) 837 WP_101895486.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 44908.70 Da        Isoelectric Point: 9.6086

>NTDB_id=241656 Kalk_RS17525 WP_101895483.1 4085567..4086823(-) (pilC) [Ketobacter alkanivorans strain GI5]
MATATQAQPKAAKKAAAPKVQTFSWEGLDKKGNKVKGEIPGQNVTLVKAELRKQGIVANKVKKKATSMLGPRKKKITAMD
IAVFTRQLATMMKAGVPLVQSFEIVADGHDNASMRELLMKIKSDIEGGNNLATALESHPAYFDDLFISLVSSGEQSGALE
TMLDRIAIYKEKTEALKAKIKKAIKYPVAVLIVAAIVTAILLIKVVPTFQDLFGSFGAELPAFTQMVINMSDWLQTWWWA
VLLILAATGIAFTEAKKRSQAFCDSLDRLALKLPVAGDIIFKATIARYARTLATTFAAGVPLVEALDSVAGATGNVVYRN
AVNKIRDDVSSGTQLNFSMKASGVFPAMAVQMTSIGEESGALDTMLEKVATYYEDEVDNAVDGLTSLLEPLIMAVLGVLV
GGLIIAMYLPIFQLGSVV

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=241656 Kalk_RS17525 WP_101895483.1 4085567..4086823(-) (pilC) [Ketobacter alkanivorans strain GI5]
ATGGCTACGGCAACTCAAGCTCAACCAAAAGCAGCCAAAAAGGCGGCAGCTCCCAAGGTACAGACATTTTCCTGGGAGGG
CCTGGATAAGAAAGGCAATAAAGTAAAGGGTGAGATCCCCGGGCAAAACGTTACCTTGGTAAAAGCCGAGCTGCGCAAAC
AGGGCATCGTGGCCAACAAGGTCAAAAAGAAAGCAACCTCAATGCTTGGGCCCCGCAAAAAGAAAATCACCGCCATGGAC
ATCGCCGTATTCACCCGGCAACTGGCAACCATGATGAAAGCCGGGGTTCCGTTGGTGCAGTCATTCGAAATCGTTGCCGA
TGGGCACGATAATGCATCCATGCGAGAGCTTTTGATGAAAATCAAAAGTGATATTGAAGGCGGTAACAACCTGGCCACCG
CACTGGAATCCCATCCCGCCTACTTCGATGATTTGTTTATCAGTTTGGTTAGCTCCGGTGAGCAGTCCGGTGCCCTTGAA
ACCATGCTGGACCGCATAGCCATATATAAAGAAAAAACCGAAGCCCTGAAAGCCAAAATCAAAAAAGCAATCAAATACCC
AGTCGCTGTACTGATCGTGGCAGCCATTGTTACCGCCATCCTGCTGATTAAGGTGGTTCCCACCTTTCAGGATCTGTTTG
GTAGCTTTGGAGCAGAGCTGCCCGCCTTTACGCAGATGGTTATCAATATGTCTGATTGGCTTCAAACCTGGTGGTGGGCT
GTATTGTTGATATTGGCCGCCACGGGCATTGCCTTCACCGAAGCAAAAAAGCGCTCTCAGGCATTCTGTGATTCATTGGA
TCGCCTCGCACTCAAGTTGCCTGTGGCCGGTGACATCATCTTTAAAGCCACTATCGCTCGCTACGCCAGAACCCTGGCCA
CCACTTTTGCGGCCGGTGTTCCCCTGGTGGAGGCACTCGACTCCGTTGCAGGTGCCACCGGTAATGTTGTTTACCGCAAC
GCGGTTAACAAGATACGCGACGACGTCTCAAGCGGTACGCAACTGAACTTCTCCATGAAGGCCTCTGGTGTATTCCCTGC
CATGGCCGTACAGATGACTTCCATCGGTGAAGAATCCGGTGCGTTAGATACCATGCTGGAAAAAGTCGCCACTTACTACG
AGGATGAGGTGGACAACGCCGTAGATGGCCTCACCAGCCTCTTGGAGCCACTGATCATGGCCGTGCTCGGCGTACTTGTT
GGCGGCCTGATCATCGCTATGTATCTCCCCATCTTCCAGTTGGGCTCGGTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)

Type II secretion system (T2SS), protein F (T2SSF)

(289-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9LP44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

66.915

96.172

0.644
  pilC Acinetobacter baumannii D1279779

65.829

95.215

0.627
  pilC Acinetobacter baylyi ADP1

62.899

97.368

0.612
  pilC Legionella pneumophila strain ERS1305867

54.975

96.172

0.529
  pilG Neisseria gonorrhoeae MS11

47.395

96.411

0.457
  pilG Neisseria meningitidis 44/76-A

47.395

96.411

0.457
  pilC Vibrio cholerae strain A1552

42.394

95.933

0.407
  pilC Vibrio campbellii strain DS40M4

40.988

96.89

0.397

Multiple sequence alignment