Detailed information NTG0241655

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Kalk_RS17520
• Genome accession: NZ_CP022684
• Coordinates: 4084642..4085550 (-)
• Length: 302 a.a.
• NCBI ID: WP_101895482.1
• Uniprot ID: A0A2K9LPE1
• Organism: Ketobacter alkanivorans strain GI5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4079642..4090550

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Kalk_RS17495 (Kalk_17470)	argJ	4081149..4082381 (+)	1233	WP_101895477.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
Kalk_RS17500 (Kalk_17475)	-	4082385..4083347 (+)	963	WP_101895478.1	Nudix family hydrolase	-
Kalk_RS17505 (Kalk_17480)	-	4083378..4083710 (+)	333	WP_101895479.1	YegP family protein	-
Kalk_RS17510 (Kalk_17485)	yacG	4083779..4083967 (-)	189	WP_101895480.1	DNA gyrase inhibitor YacG	-
Kalk_RS17515 (Kalk_17490)	coaE	4083964..4084563 (-)	600	WP_101895481.1	dephospho-CoA kinase	-
Kalk_RS17520 (Kalk_17495)	pilD	4084642..4085550 (-)	909	WP_101895482.1	A24 family peptidase	Machinery gene
Kalk_RS17525 (Kalk_17500)	pilC	4085567..4086823 (-)	1257	WP_101895483.1	type II secretion system F family protein	Machinery gene
Kalk_RS17530 (Kalk_17505)	pilB	4086839..4088536 (-)	1698	WP_101895484.1	type IV-A pilus assembly ATPase PilB	Machinery gene
Kalk_RS17535 (Kalk_17510)	-	4088904..4089383 (+)	480	WP_101895485.1	pilin	-
Kalk_RS17540 (Kalk_17515)	-	4089494..4089967 (+)	474	WP_101896382.1	pilin	-

Sequence

Protein

• Length: 302 a.a.
• Molecular weight: 34063.43 Da
• Isoelectric Point: 7.5698

>NTDB_id=241655 Kalk_RS17520 WP_101895482.1 4084642..4085550(-) (pilD) [Ketobacter alkanivorans strain GI5]
MSNQVIQYLANNPEYFTGVIFLFGLMFGSFFNVVIYRVPVMMQLEWLENCRDFIKEMFGKLPKELEAHPESLPKPPFNIA
KPDSTCPHCGHKIRWYENVPLASYVALKGKCSSCKNTISLRYPIIELVTALLSGFAAWNFGFGWETAAALAFTWALICLT
MIDFDHKLLPDQITLPLMWLGLLLNMQGMFASLEDAVIGAVAGYMCLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWMGW
QYLLLILVLSSMVGAVVGISLIVIKGRDKNIPIPFGPYLAAAGWIAFFWGEAILRQYQRFLF

Nucleotide

• Length: 909 bp

>NTDB_id=241655 Kalk_RS17520 WP_101895482.1 4084642..4085550(-) (pilD) [Ketobacter alkanivorans strain GI5]
ATGTCCAACCAAGTAATCCAATACCTCGCCAATAACCCCGAGTACTTCACCGGGGTTATTTTTTTATTCGGCCTGATGTT
CGGCAGTTTTTTTAACGTTGTGATCTACCGTGTACCGGTGATGATGCAACTGGAATGGCTGGAGAACTGCCGCGACTTCA
TCAAAGAAATGTTTGGCAAGCTGCCCAAAGAACTGGAGGCACACCCGGAGTCACTGCCCAAACCACCCTTCAATATCGCA
AAACCGGACTCCACCTGCCCCCACTGCGGCCACAAGATACGCTGGTATGAGAACGTCCCTCTGGCAAGCTATGTGGCCCT
GAAAGGCAAATGCAGCAGCTGCAAAAACACCATTTCATTGCGCTACCCTATCATCGAGCTGGTTACCGCGCTGCTTTCCG
GGTTTGCCGCCTGGAACTTTGGCTTCGGCTGGGAAACCGCCGCCGCCCTGGCGTTCACCTGGGCGCTAATCTGTCTCACC
ATGATCGACTTCGATCACAAACTACTGCCCGATCAAATCACCCTGCCGCTGATGTGGCTGGGCCTGCTGCTGAATATGCA
GGGCATGTTCGCCTCCCTTGAAGACGCTGTGATCGGCGCTGTTGCGGGCTACATGTGTCTATGGAGCCTCTATTGGGCGT
TTAAACTGCTCACTGGCAAGGAGGGCATGGGGTATGGTGACTTCAAGCTACTGGCGGCCCTGGGTGCCTGGATGGGCTGG
CAATACCTGTTACTGATTCTGGTATTATCGTCCATGGTAGGCGCGGTGGTTGGCATCAGCCTGATTGTGATAAAAGGCCG
CGACAAAAATATTCCCATCCCTTTCGGCCCCTATCTCGCTGCCGCAGGCTGGATCGCTTTCTTCTGGGGTGAAGCCATCC
TGCGCCAGTACCAACGCTTCCTGTTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (151-259)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-140)

3D structure

• AlphaFold DB: A0A2K9LPE1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.748	94.702	0.49
pilD	Vibrio campbellii strain DS40M4	49.831	97.682	0.487
pilD	Acinetobacter nosocomialis M2	46.78	97.682	0.457
pilD	Acinetobacter baumannii D1279779	46.441	97.682	0.454
pilD	Neisseria gonorrhoeae MS11	48.54	90.728	0.44