Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Kalk_RS17530 Genome accession   NZ_CP022684
Coordinates   4086839..4088536 (-) Length   565 a.a.
NCBI ID   WP_101895484.1    Uniprot ID   A0A2K9LP12
Organism   Ketobacter alkanivorans strain GI5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4081839..4093536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Kalk_RS17500 (Kalk_17475) - 4082385..4083347 (+) 963 WP_101895478.1 Nudix family hydrolase -
  Kalk_RS17505 (Kalk_17480) - 4083378..4083710 (+) 333 WP_101895479.1 YegP family protein -
  Kalk_RS17510 (Kalk_17485) yacG 4083779..4083967 (-) 189 WP_101895480.1 DNA gyrase inhibitor YacG -
  Kalk_RS17515 (Kalk_17490) coaE 4083964..4084563 (-) 600 WP_101895481.1 dephospho-CoA kinase -
  Kalk_RS17520 (Kalk_17495) pilD 4084642..4085550 (-) 909 WP_101895482.1 A24 family peptidase Machinery gene
  Kalk_RS17525 (Kalk_17500) pilC 4085567..4086823 (-) 1257 WP_101895483.1 type II secretion system F family protein Machinery gene
  Kalk_RS17530 (Kalk_17505) pilB 4086839..4088536 (-) 1698 WP_101895484.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Kalk_RS17535 (Kalk_17510) - 4088904..4089383 (+) 480 WP_101895485.1 pilin -
  Kalk_RS17540 (Kalk_17515) - 4089494..4089967 (+) 474 WP_101896382.1 pilin -
  Kalk_RS17545 (Kalk_17520) nadC 4089989..4090825 (-) 837 WP_101895486.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Kalk_RS17550 (Kalk_17525) - 4090833..4092173 (-) 1341 WP_101895487.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61618.58 Da        Isoelectric Point: 5.1683

>NTDB_id=241657 Kalk_RS17530 WP_101895484.1 4086839..4088536(-) (pilB) [Ketobacter alkanivorans strain GI5]
MNLSGLAKKLLNEGVIAEDAIRSASETAHKQKIPLVTYLVQNNLVPARTIALAASDEFGTPLIELDSMSQDAYPKELVNE
KLVRKHRALPLFKRGTRLYVAVSDPTNLQALDEIKFNTGLSTEAVVVDEDKLDGAIDRYLEGTDTGFSDLGDTDLDDLDI
EAGTDDDKGPDVTAEAADDAPIVRFVNKILLDAIKMGASDIHFEPYEKAYRIRLRADGILREVARPPVNLAPKLAARLKV
MSQLDISERRLPQDGRIKLKLSKTRAIDFRVNSLPTLFGEKIVLRILDPDSAKLGIDMLGYEEDQKDLFMKALEQPQGMI
LVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPAEINLEGINQVNVNTKVGLDFGAALRAFLRQDPDIVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLARRLCKSCKKPIDVPKETLIAEGFKEGE
IGSFTIYGPHGCDKCVNGYKGRVGIYEVVKVTDGISRIIMEEGNAIQIADAARAEGFNDLRASGLKKVKDGLTSLAEVNR
VTTGH

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=241657 Kalk_RS17530 WP_101895484.1 4086839..4088536(-) (pilB) [Ketobacter alkanivorans strain GI5]
ATGAATTTAAGTGGCCTGGCAAAAAAACTGTTAAATGAAGGGGTAATTGCCGAAGACGCTATACGCAGCGCCTCCGAAAC
AGCCCATAAGCAGAAAATCCCTCTGGTAACCTATCTGGTACAAAACAACTTGGTACCAGCCAGAACCATTGCTCTGGCGG
CTTCCGACGAATTTGGAACGCCCCTGATCGAACTGGACTCCATGTCGCAGGATGCCTACCCCAAAGAGCTGGTTAACGAA
AAGCTGGTGCGCAAACACCGGGCCCTGCCTCTGTTCAAACGCGGCACCCGGCTCTATGTGGCGGTGTCCGACCCCACCAA
TCTGCAGGCTCTGGACGAAATCAAATTCAATACCGGCCTGAGCACAGAGGCCGTGGTGGTGGATGAAGACAAGCTCGATG
GTGCCATCGACCGCTACCTGGAAGGCACCGATACTGGCTTCAGCGACCTGGGTGACACCGATCTGGATGACCTGGACATT
GAGGCCGGCACAGATGACGATAAGGGTCCAGATGTTACTGCAGAAGCAGCGGATGATGCCCCTATTGTTCGCTTTGTTAA
CAAAATTTTGTTGGACGCCATCAAAATGGGGGCGTCAGACATCCACTTTGAGCCCTATGAGAAGGCCTATCGTATTCGTC
TGCGGGCCGACGGCATCCTCCGTGAAGTGGCTCGCCCCCCCGTTAACCTGGCACCCAAGTTGGCGGCCCGCCTGAAAGTC
ATGTCACAACTGGACATATCCGAACGCCGACTGCCTCAGGATGGCAGGATCAAGCTGAAACTGTCCAAAACCCGTGCTAT
CGACTTCCGGGTGAACAGCCTGCCCACCCTGTTTGGCGAAAAGATCGTACTGCGAATCCTCGATCCGGACAGCGCGAAAC
TGGGCATCGACATGCTCGGCTACGAAGAGGACCAAAAAGACCTGTTCATGAAGGCGCTGGAACAACCTCAGGGTATGATT
CTGGTGACCGGGCCGACAGGATCAGGTAAAACCGTATCCCTTTATACCGGCCTGAATATCCTAAATACGCCAGAGCGCAA
CATCTCAACCGCGGAAGATCCGGCAGAAATCAACCTGGAAGGCATCAATCAGGTAAACGTAAACACTAAAGTAGGCCTGG
ATTTCGGTGCCGCACTAAGAGCATTCCTGCGGCAAGATCCAGACATTGTTATGGTGGGCGAGATCCGCGATCTTGAAACC
GCAGAAATTGCCATCAAAGCAGCCCAGACTGGTCACTTGGTTTTATCAACGCTCCATACCAATAGCGCACCGGAAACCTT
GACGCGTTTGCGTAACATGGGCGTGCCAGCATTCAACATCGCCACCTCGGTATCGCTGATCATCGCCCAACGACTGGCAA
GGCGTTTGTGCAAAAGCTGCAAGAAGCCCATTGATGTGCCGAAAGAAACTCTTATAGCTGAAGGTTTTAAAGAGGGCGAA
ATAGGCTCATTTACCATATATGGGCCCCATGGTTGTGACAAATGTGTGAACGGGTACAAAGGTCGAGTGGGTATATATGA
AGTAGTTAAAGTCACTGATGGCATTTCACGCATTATAATGGAAGAAGGCAACGCGATTCAGATCGCCGATGCGGCAAGAG
CAGAAGGGTTTAACGACCTTAGAGCCAGCGGACTCAAAAAAGTAAAAGATGGATTAACCAGTCTGGCAGAAGTTAACCGT
GTAACAACCGGGCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9LP12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.199

100

0.614
  pilB Acinetobacter baylyi ADP1

61.348

99.823

0.612
  pilB Legionella pneumophila strain ERS1305867

56.028

99.823

0.559
  pilB Vibrio parahaemolyticus RIMD 2210633

50.799

99.646

0.506
  pilB Vibrio cholerae strain A1552

53.89

93.274

0.503
  pilB Vibrio campbellii strain DS40M4

50.446

99.292

0.501
  pilF Neisseria gonorrhoeae MS11

48.763

100

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.602

97.876

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

40.077

91.858

0.368

Multiple sequence alignment