Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CHF41_RS05060 Genome accession   NZ_CP022680
Coordinates   996701..997339 (+) Length   212 a.a.
NCBI ID   WP_119876269.1    Uniprot ID   -
Organism   Streptococcus respiraculi strain HTS25     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 981568..999665 996701..997339 within 0


Gene organization within MGE regions


Location: 981568..999665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHF41_RS04995 - 981568..981870 (+) 303 WP_119876259.1 hypothetical protein -
  CHF41_RS05000 - 982038..986360 (+) 4323 WP_119876260.1 SpaA isopeptide-forming pilin-related protein -
  CHF41_RS05005 - 986587..986796 (+) 210 WP_240622969.1 PC4/YdbC family ssDNA-binding protein -
  CHF41_RS05010 - 986786..987007 (+) 222 WP_228065155.1 hypothetical protein -
  CHF41_RS05015 - 987023..987772 (+) 750 WP_119876262.1 hypothetical protein -
  CHF41_RS05020 - 987774..988136 (+) 363 WP_119876263.1 PrgI family protein -
  CHF41_RS05025 - 988090..990462 (+) 2373 WP_119876264.1 VirB4-like conjugal transfer ATPase, CD1110 family -
  CHF41_RS05030 - 990482..993076 (+) 2595 WP_119876265.1 phage tail tip lysozyme -
  CHF41_RS05035 - 993099..993713 (+) 615 WP_119876266.1 hypothetical protein -
  CHF41_RS05040 - 993989..994954 (-) 966 WP_119876267.1 DDE-type integrase/transposase/recombinase -
  CHF41_RS05050 - 995385..996254 (+) 870 WP_240622970.1 minor capsid protein -
  CHF41_RS05055 - 996217..996426 (+) 210 WP_119876268.1 CPCC family cysteine-rich protein -
  CHF41_RS05060 comEA 996701..997339 (+) 639 WP_119876269.1 helix-hairpin-helix domain-containing protein Machinery gene
  CHF41_RS05065 comEC/celB 997323..999665 (+) 2343 WP_119876270.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene

Sequence

Protein


Download         Length: 212 a.a.        Molecular weight: 22335.17 Da        Isoelectric Point: 4.5622

>NTDB_id=241576 CHF41_RS05060 WP_119876269.1 996701..997339(+) (comEA) [Streptococcus respiraculi strain HTS25]
MLEKWIQVLRDYKWFIVAGVGLGVILVTVFLVGQKGQTEEAISLTDFSTSSEQVVETPTDSQPTEKLVVDVKGAVKKPGI
YHLSAGSRVQDAVEAAGGLTDAADSKSVNLAQKVNDEGVVYVATKEEAVSVVPAANPVQVSGDKGATTGLVNLNTATEAE
LQTISGIGAKRAADIIAYRESNGRFQSVDDLKNVPGIGGKSLENIRSYVTVD

Nucleotide


Download         Length: 639 bp        

>NTDB_id=241576 CHF41_RS05060 WP_119876269.1 996701..997339(+) (comEA) [Streptococcus respiraculi strain HTS25]
ATGCTTGAAAAGTGGATACAAGTTTTGCGTGATTATAAGTGGTTTATTGTGGCAGGAGTAGGGCTTGGTGTTATTCTTGT
GACCGTCTTTTTAGTGGGACAAAAGGGGCAGACAGAAGAAGCGATTAGTCTGACAGACTTTTCAACTAGTAGCGAGCAGG
TGGTAGAGACACCAACGGATTCGCAGCCAACTGAGAAGCTAGTAGTGGATGTCAAGGGTGCTGTCAAGAAGCCAGGCATT
TATCATCTATCAGCAGGAAGTCGTGTCCAGGATGCGGTAGAAGCAGCGGGAGGTTTGACAGATGCTGCGGATTCTAAGTC
AGTTAATCTGGCACAGAAAGTGAACGATGAGGGCGTTGTTTATGTCGCGACCAAGGAGGAAGCGGTCTCGGTTGTGCCTG
CTGCCAATCCAGTCCAAGTAAGCGGAGACAAGGGAGCAACGACAGGTCTTGTCAATCTCAATACGGCTACCGAAGCGGAA
TTGCAAACCATTTCAGGTATCGGTGCCAAGCGTGCGGCAGATATTATTGCCTACCGTGAGAGTAATGGCCGTTTTCAATC
GGTTGATGATTTGAAGAATGTACCTGGAATTGGTGGCAAGAGTTTGGAGAATATTCGTTCCTATGTCACAGTTGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(150-210)

SLBB domain (SLBB)

(68-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Streptococcus thermophilus LMD-9

45.69

100

0.5
  comEA/celA/cilE Streptococcus mitis NCTC 12261

46.018

100

0.491
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

46.222

100

0.491
  comEA/celA/cilE Streptococcus pneumoniae R6

46.396

100

0.486
  comEA/celA/cilE Streptococcus pneumoniae Rx1

46.396

100

0.486
  comEA/celA/cilE Streptococcus pneumoniae D39

46.396

100

0.486
  comEA/celA/cilE Streptococcus mitis SK321

45.045

100

0.472
  comEA Bacillus subtilis subsp. subtilis str. 168

40.952

99.057

0.406
  comEA Lactococcus lactis subsp. cremoris KW2

37.443

100

0.387
  comEA Latilactobacillus sakei subsp. sakei 23K

33.191

100

0.368

Multiple sequence alignment