Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   CGZ77_RS05020 Genome accession   NZ_CP022527
Coordinates   1017537..1018760 (-) Length   407 a.a.
NCBI ID   WP_009425282.1    Uniprot ID   L1NXF1
Organism   Neisseria sp. KEM232     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1012537..1023760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ77_RS04990 (CGZ77_04995) - 1012611..1013987 (+) 1377 WP_009427222.1 phosphomannomutase/phosphoglucomutase -
  CGZ77_RS12030 - 1014028..1014231 (+) 204 WP_157697515.1 hypothetical protein -
  CGZ77_RS04995 (CGZ77_05000) - 1014340..1014948 (-) 609 WP_009425277.1 HI_0552 family protein -
  CGZ77_RS05000 (CGZ77_05005) - 1015073..1015270 (-) 198 WP_009425278.1 DNA gyrase inhibitor YacG -
  CGZ77_RS05005 (CGZ77_05010) zapD 1015271..1016032 (-) 762 WP_009425279.1 cell division protein ZapD -
  CGZ77_RS05010 (CGZ77_05015) coaE 1016029..1016649 (-) 621 WP_009425280.1 dephospho-CoA kinase -
  CGZ77_RS05015 (CGZ77_05020) pilD 1016646..1017509 (-) 864 WP_009425281.1 A24 family peptidase Machinery gene
  CGZ77_RS05020 (CGZ77_05025) pilG 1017537..1018760 (-) 1224 WP_009425282.1 type II secretion system F family protein Machinery gene
  CGZ77_RS05025 (CGZ77_05030) pilF 1018778..1020451 (-) 1674 WP_009425283.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CGZ77_RS05030 (CGZ77_05035) - 1020591..1022180 (-) 1590 WP_009425284.1 inorganic phosphate transporter -
  CGZ77_RS05035 (CGZ77_05040) - 1022399..1023271 (-) 873 WP_009425286.1 DMT family transporter -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44753.79 Da        Isoelectric Point: 10.0025

>NTDB_id=240312 CGZ77_RS05020 WP_009425282.1 1017537..1018760(-) (pilG) [Neisseria sp. KEM232]
MKANKVNNEKGKRFTFEGKHTQSEQIVRGEVVAANEEEARKKLLRRGIKPLRITQVKATRKKRITQEDITVFTRQLATMM
KAGLPLMQAFEIVARGHSNPSMTQLLMSIRSDVEQGTSMGAAFAKHPKYFDRFYCNLVAAGEAGGVLENLLDKLAVYKEK
TQSIKKKVKSALTYPIAIVVVAVVLVIIMMMFVLPAFGKVYHDMGAELPFLTQVMMNISDFFVSYGWVIIIGLIGMVVAL
VHFHKNSPKFQKRVDAWVLKLPIFGNIVRKATIARWARTTASLFAAGVPLVEALDSVAGASGNILYEEGTITIKSQVNQG
LSLTSGMQSTGLFPNMVIQMASIGEESGSLDDMLNKVAEFYEEEVDTAVAQLSSLMEPIIMVVLGSIIGVILVAMYLPLF
NLGNVVG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=240312 CGZ77_RS05020 WP_009425282.1 1017537..1018760(-) (pilG) [Neisseria sp. KEM232]
ATGAAAGCAAATAAAGTCAATAATGAAAAAGGTAAACGGTTTACTTTTGAAGGTAAGCACACTCAGAGCGAACAAATCGT
CCGCGGAGAAGTAGTAGCTGCCAACGAGGAAGAAGCGCGTAAGAAACTGCTGCGCAGAGGTATCAAACCGTTGCGGATTA
CGCAGGTCAAAGCCACGCGCAAAAAGCGCATTACTCAGGAAGATATCACGGTTTTTACCCGTCAGTTGGCAACTATGATG
AAGGCCGGTTTGCCGTTGATGCAGGCTTTTGAAATTGTGGCGCGCGGACACTCGAATCCGTCGATGACGCAGTTGCTGAT
GTCTATCAGAAGCGATGTGGAGCAGGGCACGTCTATGGGCGCCGCTTTTGCCAAGCATCCGAAATATTTTGACCGTTTCT
ACTGCAATCTGGTTGCTGCGGGTGAGGCTGGTGGTGTGTTGGAAAACTTGTTGGACAAATTGGCTGTCTACAAGGAAAAA
ACCCAGTCTATCAAGAAAAAAGTGAAATCAGCATTGACTTATCCGATTGCCATTGTTGTTGTGGCAGTTGTATTGGTTAT
TATTATGATGATGTTTGTGTTGCCGGCATTCGGAAAAGTTTATCACGACATGGGGGCGGAACTGCCTTTCCTTACCCAGG
TTATGATGAATATTTCTGACTTTTTTGTCAGTTATGGATGGGTAATTATTATAGGACTGATCGGTATGGTGGTTGCCTTG
GTTCATTTTCATAAAAATTCTCCCAAGTTTCAGAAAAGGGTGGATGCTTGGGTGTTGAAATTACCAATATTTGGTAACAT
TGTGCGTAAAGCCACTATTGCCCGATGGGCAAGGACAACCGCCAGCCTTTTTGCCGCTGGTGTGCCTTTGGTTGAAGCTT
TGGATTCTGTGGCAGGTGCTTCAGGTAATATTTTGTACGAAGAGGGTACAATTACAATTAAATCCCAAGTAAACCAAGGT
CTGTCGTTGACTTCGGGCATGCAAAGTACGGGTTTGTTCCCCAATATGGTCATACAGATGGCATCTATCGGTGAAGAGTC
CGGTTCCTTAGACGACATGTTGAACAAGGTAGCAGAGTTTTATGAGGAAGAAGTCGACACAGCTGTTGCCCAGCTGTCTT
CACTGATGGAACCGATTATTATGGTGGTATTGGGCAGCATTATTGGTGTGATATTGGTAGCCATGTACCTGCCGCTGTTC
AACTTGGGTAACGTAGTAGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L1NXF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

68.844

97.789

0.673
  pilG Neisseria meningitidis 44/76-A

68.342

97.789

0.668
  pilC Legionella pneumophila strain ERS1305867

47.99

97.789

0.469
  pilC Acinetobacter baumannii D1279779

45.729

97.789

0.447
  pilC Pseudomonas stutzeri DSM 10701

45.5

98.28

0.447
  pilC Acinetobacter baylyi ADP1

45.363

98.034

0.445
  pilC Vibrio cholerae strain A1552

38.5

98.28

0.378
  pilC Vibrio campbellii strain DS40M4

36.919

100

0.371

Multiple sequence alignment