Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CGZ77_RS05015 Genome accession   NZ_CP022527
Coordinates   1016646..1017509 (-) Length   287 a.a.
NCBI ID   WP_009425281.1    Uniprot ID   L1NWY0
Organism   Neisseria sp. KEM232     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1011646..1022509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ77_RS04990 (CGZ77_04995) - 1012611..1013987 (+) 1377 WP_009427222.1 phosphomannomutase/phosphoglucomutase -
  CGZ77_RS12030 - 1014028..1014231 (+) 204 WP_157697515.1 hypothetical protein -
  CGZ77_RS04995 (CGZ77_05000) - 1014340..1014948 (-) 609 WP_009425277.1 HI_0552 family protein -
  CGZ77_RS05000 (CGZ77_05005) - 1015073..1015270 (-) 198 WP_009425278.1 DNA gyrase inhibitor YacG -
  CGZ77_RS05005 (CGZ77_05010) zapD 1015271..1016032 (-) 762 WP_009425279.1 cell division protein ZapD -
  CGZ77_RS05010 (CGZ77_05015) coaE 1016029..1016649 (-) 621 WP_009425280.1 dephospho-CoA kinase -
  CGZ77_RS05015 (CGZ77_05020) pilD 1016646..1017509 (-) 864 WP_009425281.1 A24 family peptidase Machinery gene
  CGZ77_RS05020 (CGZ77_05025) pilG 1017537..1018760 (-) 1224 WP_009425282.1 type II secretion system F family protein Machinery gene
  CGZ77_RS05025 (CGZ77_05030) pilF 1018778..1020451 (-) 1674 WP_009425283.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CGZ77_RS05030 (CGZ77_05035) - 1020591..1022180 (-) 1590 WP_009425284.1 inorganic phosphate transporter -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31296.27 Da        Isoelectric Point: 8.5788

>NTDB_id=240311 CGZ77_RS05015 WP_009425281.1 1016646..1017509(-) (pilD) [Neisseria sp. KEM232]
MTEYYAPLVIPISVILGLLVGSFLNVVIYRVPVMMERGWTLFSKEHLGIELTDEEKQPFSLTRPPSRCPKCGNRVRPWQN
IPVVSYLLLKGKCGHCHTHISARYPAVEVLTAVVFGIVAACYGWSWITLTGLVFTGFLVALAFIDADTQYLPDELTIPLV
WLGLLAAYGGSGLVTLQQSVMGAVCGYLSLWLLNRLHKQIRGIDGMGGGDFKLLAALGAWLGAAVLPVVVFVAAVVGIVS
ALIMKVRQSQPMAFGPCLIIAGWIVFVTHDKVLAGVRWWLHMSGFSA

Nucleotide


Download         Length: 864 bp        

>NTDB_id=240311 CGZ77_RS05015 WP_009425281.1 1016646..1017509(-) (pilD) [Neisseria sp. KEM232]
ATGACGGAATATTATGCCCCTTTGGTCATTCCCATCTCGGTAATTCTCGGCCTGCTGGTCGGTAGTTTTCTCAATGTCGT
GATTTACCGCGTTCCCGTGATGATGGAACGCGGGTGGACTCTGTTTTCCAAAGAGCATTTGGGAATCGAGCTGACCGATG
AGGAAAAGCAGCCGTTCAGTCTGACCCGGCCGCCGTCGCGCTGTCCGAAATGCGGTAACCGGGTCAGGCCGTGGCAGAAC
ATACCGGTTGTCAGCTACTTGCTGTTGAAAGGCAAATGCGGCCATTGCCATACCCATATCAGTGCCCGTTATCCCGCTGT
CGAAGTATTGACGGCCGTTGTGTTCGGCATTGTTGCCGCTTGTTACGGCTGGTCTTGGATAACGCTGACAGGCTTGGTGT
TTACGGGTTTCTTGGTGGCTTTGGCCTTTATCGATGCGGATACGCAATATCTGCCTGACGAGCTGACCATTCCTCTGGTT
TGGCTGGGTTTGCTTGCGGCTTATGGCGGCAGCGGCTTGGTTACCTTGCAGCAGTCTGTCATGGGCGCGGTATGCGGCTA
TCTCAGCTTATGGCTGTTAAACCGGCTTCATAAGCAGATTCGCGGAATTGACGGCATGGGCGGAGGCGATTTCAAGCTTT
TGGCCGCATTGGGTGCGTGGCTTGGCGCGGCGGTTTTGCCCGTTGTCGTTTTTGTTGCGGCCGTTGTCGGTATTGTTTCT
GCCCTCATTATGAAAGTCAGACAAAGCCAGCCGATGGCTTTCGGCCCCTGCCTGATTATTGCCGGCTGGATTGTTTTCGT
CACGCATGACAAAGTTCTGGCAGGCGTGCGCTGGTGGCTGCATATGTCGGGTTTTTCGGCATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(132-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(15-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L1NWY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

60

95.819

0.575
  pilD Vibrio campbellii strain DS40M4

45.455

95.819

0.436
  pilD Vibrio cholerae strain A1552

44

95.819

0.422
  pilD Acinetobacter baumannii D1279779

44.828

90.941

0.408
  pilD Acinetobacter nosocomialis M2

44.444

90.941

0.404

Multiple sequence alignment