Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CGZ77_RS05025 Genome accession   NZ_CP022527
Coordinates   1018778..1020451 (-) Length   557 a.a.
NCBI ID   WP_009425283.1    Uniprot ID   -
Organism   Neisseria sp. KEM232     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1013778..1025451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ77_RS12030 - 1014028..1014231 (+) 204 WP_157697515.1 hypothetical protein -
  CGZ77_RS04995 (CGZ77_05000) - 1014340..1014948 (-) 609 WP_009425277.1 HI_0552 family protein -
  CGZ77_RS05000 (CGZ77_05005) - 1015073..1015270 (-) 198 WP_009425278.1 DNA gyrase inhibitor YacG -
  CGZ77_RS05005 (CGZ77_05010) zapD 1015271..1016032 (-) 762 WP_009425279.1 cell division protein ZapD -
  CGZ77_RS05010 (CGZ77_05015) coaE 1016029..1016649 (-) 621 WP_009425280.1 dephospho-CoA kinase -
  CGZ77_RS05015 (CGZ77_05020) pilD 1016646..1017509 (-) 864 WP_009425281.1 A24 family peptidase Machinery gene
  CGZ77_RS05020 (CGZ77_05025) pilG 1017537..1018760 (-) 1224 WP_009425282.1 type II secretion system F family protein Machinery gene
  CGZ77_RS05025 (CGZ77_05030) pilF 1018778..1020451 (-) 1674 WP_009425283.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CGZ77_RS05030 (CGZ77_05035) - 1020591..1022180 (-) 1590 WP_009425284.1 inorganic phosphate transporter -
  CGZ77_RS05035 (CGZ77_05040) - 1022399..1023271 (-) 873 WP_009425286.1 DMT family transporter -
  CGZ77_RS05040 (CGZ77_05045) - 1023719..1024294 (+) 576 WP_009425290.1 DUF1415 domain-containing protein -
  CGZ77_RS05045 (CGZ77_05050) - 1024294..1024653 (+) 360 WP_232304371.1 YbaN family protein -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 61982.15 Da        Isoelectric Point: 5.5695

>NTDB_id=240313 CGZ77_RS05025 WP_009425283.1 1018778..1020451(-) (pilF) [Neisseria sp. KEM232]
MSLGLLRVLVQNRILSNEQTGRYQKALGENKDIIPMLFGDNITNPKALAELLAQIFNYPVLDLSYYPRSNIVQDVLPEEQ
MQANACVPVFKRGNRVFLAVSDPTKIQEFQKLVTGYTIDLVVVRHDQLMTLVEWLGQRSTTILREMSQDQLDTSTSQALF
IGDEEAEDGPIARFIHKTLSDALNSGASDIHFEFYENTARVRFRVDGQLREVVKPPVTVRSQLASRIKVMAKLDISEKRV
PQDGRIQIAFSKHGRPTDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEDFQKDLLLEAIHRPYGMVLVTGPTGSGK
TVSLYTCLNILNTEGVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKASQT
GHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKEPMDRPPEHALKKAGFTDEDLAREWTMYR
AVGCDTCRGKGYKGRAGVYEVMPITGEMQRVIMEGGTEVDIQQMAYQEGMVDLRRAGLLKVMDGITSLEEVLGNTND

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=240313 CGZ77_RS05025 WP_009425283.1 1018778..1020451(-) (pilF) [Neisseria sp. KEM232]
ATGAGTTTAGGTTTGTTAAGGGTTTTAGTGCAAAACCGTATACTGTCCAACGAACAGACGGGGCGCTATCAGAAAGCTTT
AGGGGAAAATAAAGACATTATCCCCATGCTGTTTGGCGATAATATTACCAATCCGAAAGCGCTGGCGGAATTGTTGGCTC
AGATTTTCAATTATCCGGTTTTGGATTTGAGTTATTACCCGCGCAGCAATATCGTGCAGGATGTGTTACCGGAAGAGCAG
ATGCAGGCCAACGCCTGCGTGCCGGTATTCAAACGCGGAAACAGGGTGTTTCTGGCAGTTTCCGATCCGACCAAAATTCA
GGAATTTCAAAAACTGGTAACCGGGTATACGATTGATTTGGTTGTCGTACGACATGACCAGCTGATGACTTTGGTCGAGT
GGCTGGGTCAGCGCTCCACCACTATTTTGCGCGAGATGTCGCAAGATCAGTTGGATACCTCGACTTCTCAGGCATTGTTT
ATCGGCGATGAAGAAGCAGAAGACGGGCCGATTGCACGTTTCATCCACAAAACGCTTTCTGATGCCCTTAATTCGGGCGC
GTCGGATATCCACTTTGAGTTTTATGAAAATACGGCAAGGGTTCGTTTCCGTGTCGACGGCCAGCTTAGGGAGGTTGTCA
AACCTCCTGTGACCGTACGAAGCCAGCTCGCATCGCGCATCAAAGTGATGGCCAAACTCGATATTTCCGAGAAGCGCGTC
CCTCAAGACGGCCGTATTCAGATTGCGTTTTCCAAACATGGCCGCCCCACGGATTTCCGTGTCAGCACACTGCCTACCCT
TTTTGGCGAAAAAGTGGTGATGCGTATTTTGAATTCCGATGCGGCATCGCTCAATATAGATCAACTCGGTTTTGAGGATT
TCCAAAAAGATTTGCTGTTGGAGGCAATTCACAGGCCTTACGGCATGGTTTTGGTTACCGGACCGACCGGTTCGGGTAAA
ACCGTCTCGTTGTATACATGCCTGAATATTCTGAATACCGAAGGTGTGAATATTTCTACCGCAGAGGATCCAGCCGAGAT
TAACCTGCCCGGCATTAACCAGGTAAACGTAAACGACAAACAGGGACTGACCTTTGCAGCAGCACTGAAATCGTTTCTCC
GTCAAGACCCGGATATCATCATGGTCGGTGAGATTCGCGATTTGGAGACGGCGGATATTGCAATTAAAGCCTCGCAAACC
GGTCACATGGTGTTCTCAACGCTGCATACCAATAATGCGCCGGCAACTTTGTCCCGTATGTTGAATATGGGTGTCGCGCC
GTTCAATATTGCCAGCTCGGTTAGTCTGATTATGGCGCAGAGGCTGCTCCGCCGCCTGTGTCCGAACTGCAAAGAGCCAA
TGGACAGGCCGCCTGAACATGCATTAAAAAAAGCCGGTTTTACCGATGAAGATTTGGCACGTGAATGGACGATGTATCGT
GCGGTCGGCTGCGACACATGCCGCGGTAAAGGTTACAAAGGCCGAGCCGGTGTTTATGAAGTTATGCCGATAACCGGTGA
AATGCAGAGGGTAATTATGGAAGGTGGTACGGAAGTCGATATACAGCAGATGGCCTATCAGGAGGGTATGGTCGATTTGC
GCAGAGCAGGTTTGTTGAAGGTAATGGATGGGATTACTTCTTTGGAAGAAGTATTGGGTAACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(177-445)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(9-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

74.061

100

0.743
  pilB Acinetobacter baumannii D1279779

51.246

100

0.517
  pilB Acinetobacter baylyi ADP1

50.899

99.82

0.508
  pilB Legionella pneumophila strain ERS1305867

48.485

100

0.488
  pilB Vibrio cholerae strain A1552

47.425

100

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

100

0.46
  pilB Vibrio campbellii strain DS40M4

45.455

94.794

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.927

92.998

0.381

Multiple sequence alignment