Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CEK71_RS05355 Genome accession   NZ_CP022129
Coordinates   1192594..1193814 (+) Length   406 a.a.
NCBI ID   WP_088618415.1    Uniprot ID   A0A1Z4BW84
Organism   Methylovulum psychrotolerans strain HV10_M2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1187594..1198814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEK71_RS05345 (CEK71_05345) - 1187975..1190770 (+) 2796 WP_088618413.1 valine--tRNA ligase -
  CEK71_RS05350 (CEK71_05350) pilB 1190882..1192591 (+) 1710 WP_088618414.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CEK71_RS05355 (CEK71_05355) pilC 1192594..1193814 (+) 1221 WP_088618415.1 type II secretion system F family protein Machinery gene
  CEK71_RS05360 (CEK71_05360) pilD 1193834..1194706 (+) 873 WP_088618416.1 A24 family peptidase Machinery gene
  CEK71_RS05365 (CEK71_05365) coaE 1194711..1195313 (+) 603 WP_088618417.1 dephospho-CoA kinase -
  CEK71_RS05370 (CEK71_05370) zapD 1195321..1196091 (+) 771 WP_088618418.1 cell division protein ZapD -
  CEK71_RS05375 (CEK71_05375) yacG 1196091..1196303 (+) 213 WP_088618419.1 DNA gyrase inhibitor YacG -
  CEK71_RS05380 (CEK71_05380) - 1196482..1197417 (-) 936 WP_088618420.1 Nudix family hydrolase -
  CEK71_RS05385 (CEK71_05385) argJ 1197436..1198659 (-) 1224 WP_088618421.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44697.95 Da        Isoelectric Point: 8.8237

>NTDB_id=237068 CEK71_RS05355 WP_088618415.1 1192594..1193814(+) (pilC) [Methylovulum psychrotolerans strain HV10_M2]
MAEKTDQIDFVWQGLDKVKNKTKGEISAISEVVAKAELRRQGFRVTSIKKKAKPLFGARPKPITPGDIAIFARQLATMMG
AGLPLVQAIDIVGRGHENPSMQAMLMKIKGDIEAGDTLAEALQRHPIQFDELFCNLVRAGEQAGVLESLLDKVATYKEKS
ESMKKKIKKALTYPIAVLVVAFLVTAILLLFVVPVFEDLFKSFGADLPAFTRMVIDMSAWLQAWWYVVLGILLGAYYTFD
FFKKRSKAFNHFLDRMLLQVPVVGLIINKSAIARFARTLSTMSAAGLPLVEALVSVAGSCGNIIYAEAVMKMREDVANGQ
RLQFAMQQTGIWPHMVQQMVAIGEEAGSMDAMLSKVADFYEEEVDNLVDNLSSLMEPIIMVILGILVGGLIVAMYLPIFK
LGAVVS

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=237068 CEK71_RS05355 WP_088618415.1 1192594..1193814(+) (pilC) [Methylovulum psychrotolerans strain HV10_M2]
ATGGCAGAGAAAACCGACCAAATTGATTTTGTCTGGCAAGGTCTGGACAAGGTAAAAAATAAAACCAAAGGCGAAATTTC
CGCCATCAGCGAAGTGGTCGCCAAAGCCGAATTACGGCGGCAGGGCTTTCGCGTCACCAGCATCAAGAAAAAAGCCAAAC
CGCTGTTCGGCGCCCGGCCAAAACCCATCACACCCGGCGACATCGCCATTTTTGCCCGCCAATTGGCGACCATGATGGGG
GCAGGTTTACCCTTAGTACAGGCGATTGACATCGTCGGGCGCGGCCACGAAAACCCCAGTATGCAAGCCATGCTGATGAA
GATAAAAGGCGATATTGAGGCGGGCGATACCTTAGCAGAGGCCTTGCAACGGCATCCTATCCAGTTTGACGAACTGTTTT
GCAACTTGGTGCGGGCAGGTGAACAAGCCGGTGTATTGGAATCCTTACTCGATAAGGTCGCCACTTATAAAGAAAAATCC
GAGTCCATGAAAAAGAAAATCAAAAAGGCCCTCACCTACCCTATCGCCGTATTGGTGGTGGCGTTTTTGGTGACGGCCAT
TTTGCTGTTGTTTGTGGTGCCGGTGTTCGAAGACTTGTTCAAAAGCTTTGGTGCAGACTTACCGGCCTTTACCCGTATGG
TCATCGACATGTCGGCATGGTTGCAAGCGTGGTGGTATGTGGTACTGGGGATTTTGCTCGGCGCTTATTACACCTTTGAT
TTTTTCAAAAAACGCTCCAAGGCCTTCAACCATTTTCTTGACCGGATGCTCTTGCAAGTTCCCGTCGTCGGATTGATTAT
CAACAAATCCGCGATCGCCCGCTTTGCGCGTACCTTATCCACCATGTCCGCCGCCGGTTTACCGCTGGTTGAGGCATTAG
TCTCCGTAGCAGGGTCTTGCGGTAACATTATTTATGCCGAAGCCGTTATGAAAATGCGTGAAGATGTCGCCAACGGCCAG
CGCTTACAGTTTGCCATGCAGCAGACCGGAATATGGCCGCACATGGTGCAGCAAATGGTCGCCATTGGTGAAGAGGCCGG
CTCTATGGATGCCATGCTATCGAAAGTAGCCGACTTTTACGAAGAAGAAGTGGATAACTTGGTAGACAATTTAAGCAGCC
TGATGGAACCCATTATCATGGTCATCTTGGGTATTCTGGTCGGCGGCCTGATTGTCGCCATGTATCTGCCGATCTTCAAG
CTCGGCGCTGTCGTCAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z4BW84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

61.558

98.03

0.603
  pilC Pseudomonas stutzeri DSM 10701

56.818

97.537

0.554
  pilC Acinetobacter baumannii D1279779

54.412

100

0.547
  pilC Acinetobacter baylyi ADP1

53.431

100

0.537
  pilG Neisseria meningitidis 44/76-A

47.132

98.768

0.466
  pilG Neisseria gonorrhoeae MS11

47.132

98.768

0.466
  pilC Vibrio cholerae strain A1552

40.302

97.783

0.394
  pilC Vibrio campbellii strain DS40M4

39.798

97.783

0.389
  pilC Thermus thermophilus HB27

37.717

99.261

0.374

Multiple sequence alignment