Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CEK71_RS05360 Genome accession   NZ_CP022129
Coordinates   1193834..1194706 (+) Length   290 a.a.
NCBI ID   WP_088618416.1    Uniprot ID   A0A1Z4BWD2
Organism   Methylovulum psychrotolerans strain HV10_M2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1188834..1199706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEK71_RS05350 (CEK71_05350) pilB 1190882..1192591 (+) 1710 WP_088618414.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CEK71_RS05355 (CEK71_05355) pilC 1192594..1193814 (+) 1221 WP_088618415.1 type II secretion system F family protein Machinery gene
  CEK71_RS05360 (CEK71_05360) pilD 1193834..1194706 (+) 873 WP_088618416.1 A24 family peptidase Machinery gene
  CEK71_RS05365 (CEK71_05365) coaE 1194711..1195313 (+) 603 WP_088618417.1 dephospho-CoA kinase -
  CEK71_RS05370 (CEK71_05370) zapD 1195321..1196091 (+) 771 WP_088618418.1 cell division protein ZapD -
  CEK71_RS05375 (CEK71_05375) yacG 1196091..1196303 (+) 213 WP_088618419.1 DNA gyrase inhibitor YacG -
  CEK71_RS05380 (CEK71_05380) - 1196482..1197417 (-) 936 WP_088618420.1 Nudix family hydrolase -
  CEK71_RS05385 (CEK71_05385) argJ 1197436..1198659 (-) 1224 WP_088618421.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31871.20 Da        Isoelectric Point: 7.2750

>NTDB_id=237069 CEK71_RS05360 WP_088618416.1 1193834..1194706(+) (pilD) [Methylovulum psychrotolerans strain HV10_M2]
MIELLDILFRSPLLCTALAGVVGLLVGSFLNVVIYRLPVMMQRSWRKECLEYLELPPEPEAAPFNLALPPSRCPACDAPI
KAYQNIPVVSYLVLQGKCAHCRNPIALRYPLLEAFTALLSIAVAWHFGYSLQTVFALLLTWALVALSFIDIDHQLLPDSI
TLPMLWLGLLLNVFGVFTDSHASIIGAAAGYLALWSVFQLFKLLTGKEGMGYGDFKLLALFGAWLGWQVLPLIILLSSLV
GTVVSIAVLLIAKRDHRIPIPFGPYLALAGWIALLWGTDINKLYLAVSGL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=237069 CEK71_RS05360 WP_088618416.1 1193834..1194706(+) (pilD) [Methylovulum psychrotolerans strain HV10_M2]
ATGATAGAGCTCCTAGACATATTATTCCGCTCGCCCTTGCTCTGTACGGCATTGGCAGGCGTGGTCGGCTTGCTGGTAGG
CAGCTTCCTGAACGTCGTCATCTACCGCCTGCCGGTCATGATGCAGCGCAGTTGGCGCAAGGAATGCCTGGAATATCTGG
AATTGCCGCCTGAACCTGAGGCGGCACCATTCAACTTGGCCTTGCCGCCCTCACGCTGCCCGGCTTGCGATGCCCCAATA
AAAGCCTACCAAAACATTCCGGTAGTCAGTTATCTCGTCTTACAAGGTAAATGCGCCCACTGCCGCAACCCCATTGCCTT
GCGTTACCCGCTCTTAGAAGCCTTCACCGCCCTACTGTCAATCGCTGTCGCTTGGCATTTCGGCTATAGCCTTCAAACCG
TGTTTGCACTGTTACTGACTTGGGCCTTAGTCGCCTTGAGCTTTATTGATATCGACCACCAATTGCTGCCCGACTCGATT
ACCCTGCCCATGCTATGGCTAGGCCTGCTGCTGAACGTTTTTGGCGTGTTTACCGATAGCCATGCCAGCATTATCGGGGC
GGCGGCGGGTTATTTGGCGTTGTGGTCGGTCTTCCAACTGTTTAAACTGCTGACGGGCAAAGAAGGTATGGGTTATGGCG
ACTTTAAGCTGCTGGCCTTATTCGGTGCTTGGCTGGGCTGGCAAGTATTGCCGTTAATCATCCTCTTATCGTCGCTGGTC
GGTACTGTCGTCAGCATTGCCGTGCTGCTCATCGCCAAACGCGACCACCGCATCCCCATACCCTTCGGGCCTTATTTGGC
CTTGGCGGGCTGGATTGCCTTATTATGGGGCACGGACATCAATAAGCTGTATTTGGCCGTATCCGGCTTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(138-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z4BWD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.389

99.31

0.51
  pilD Vibrio campbellii strain DS40M4

51.761

97.931

0.507
  pilD Acinetobacter baumannii D1279779

53.358

92.414

0.493
  pilD Acinetobacter nosocomialis M2

52.985

92.414

0.49
  pilD Neisseria gonorrhoeae MS11

53.906

88.276

0.476

Multiple sequence alignment