Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CEK71_RS05350 Genome accession   NZ_CP022129
Coordinates   1190882..1192591 (+) Length   569 a.a.
NCBI ID   WP_088618414.1    Uniprot ID   -
Organism   Methylovulum psychrotolerans strain HV10_M2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1185882..1197591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEK71_RS05335 (CEK71_05335) - 1186026..1187516 (+) 1491 WP_088618411.1 leucyl aminopeptidase -
  CEK71_RS05340 (CEK71_05340) - 1187523..1187951 (+) 429 WP_088618412.1 DNA polymerase III subunit chi -
  CEK71_RS05345 (CEK71_05345) - 1187975..1190770 (+) 2796 WP_088618413.1 valine--tRNA ligase -
  CEK71_RS05350 (CEK71_05350) pilB 1190882..1192591 (+) 1710 WP_088618414.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CEK71_RS05355 (CEK71_05355) pilC 1192594..1193814 (+) 1221 WP_088618415.1 type II secretion system F family protein Machinery gene
  CEK71_RS05360 (CEK71_05360) pilD 1193834..1194706 (+) 873 WP_088618416.1 A24 family peptidase Machinery gene
  CEK71_RS05365 (CEK71_05365) coaE 1194711..1195313 (+) 603 WP_088618417.1 dephospho-CoA kinase -
  CEK71_RS05370 (CEK71_05370) zapD 1195321..1196091 (+) 771 WP_088618418.1 cell division protein ZapD -
  CEK71_RS05375 (CEK71_05375) yacG 1196091..1196303 (+) 213 WP_088618419.1 DNA gyrase inhibitor YacG -
  CEK71_RS05380 (CEK71_05380) - 1196482..1197417 (-) 936 WP_088618420.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62652.65 Da        Isoelectric Point: 5.9170

>NTDB_id=237067 CEK71_RS05350 WP_088618414.1 1190882..1192591(+) (pilB) [Methylovulum psychrotolerans strain HV10_M2]
MATEPTDIQLRGLAKLLVQEGLLTESQARNNIKEAKENKVQLISHLVANKVVDSNTIAQKASVEFGLPFLDLDAVDCHSL
PIALINEKLIRKHHVLPLLQRGKTLFIAVSDPTNHLGVEDIKFQTLMRPELILVEESKLSRAIEIALEVPETTMMDLLDS
DLDNVNLGADDGAVVVDGNSDADEAPIVRFVNKVLLDAIKKGASDIHMEPYEKNFRIRFRQDGMLYAIASPPVNISTRII
SRVKVMSRMDIAERRVPQDGRIKLLLSKTRAIDFRVNTCPTLFGEKVVLRILDPTAAQLGIEKLGFEPEQQQLFLKAIHK
PYGLVLVTGPTGSGKTVSLYTGLNILNSIDRNISTAEDPVEITVEGINQVNVNNKAGLTFAAALKAFLRQDPDVIMVGEI
RDLETADIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGVAPFNIVSAVNLIMAQRLARRLCERCKAPIKFPDKLLLEAG
FTEEELPDLQLFAPVGCDNCTNGYKGRVGIYQVMTLSEKMCELILEGGNSLQLTQLAKAEGINDLRASGLNKVRMGVTSL
QEIDRVTKE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=237067 CEK71_RS05350 WP_088618414.1 1190882..1192591(+) (pilB) [Methylovulum psychrotolerans strain HV10_M2]
ATGGCTACTGAACCGACTGACATTCAACTAAGAGGGCTTGCCAAATTATTGGTCCAAGAAGGGCTGCTCACGGAAAGCCA
AGCCAGAAACAACATTAAAGAAGCCAAAGAAAATAAAGTCCAACTGATTAGTCACTTAGTCGCCAATAAGGTTGTTGACA
GCAACACCATCGCCCAAAAAGCGTCTGTTGAATTTGGCCTGCCGTTTTTGGATTTGGATGCGGTTGACTGCCATAGTTTA
CCCATTGCCTTAATTAACGAAAAACTCATCCGCAAACACCATGTCTTACCGCTATTGCAACGTGGCAAGACTTTATTTAT
TGCGGTATCCGACCCGACCAACCATTTGGGTGTGGAAGATATCAAGTTTCAGACGTTGATGCGTCCGGAACTGATTCTGG
TTGAAGAAAGTAAACTCAGCCGCGCCATTGAAATTGCTTTGGAAGTCCCCGAAACCACCATGATGGATTTGCTGGACTCG
GACTTGGACAATGTTAACTTAGGGGCCGACGATGGGGCGGTAGTGGTTGACGGCAATTCGGATGCCGACGAGGCACCGAT
CGTCCGGTTCGTCAATAAAGTGCTGTTGGACGCCATCAAAAAAGGCGCGTCCGACATCCACATGGAACCTTACGAAAAAA
ACTTCCGGATCCGCTTCCGGCAAGACGGTATGCTCTACGCCATCGCCAGCCCGCCCGTTAATATCTCCACGCGCATCATC
TCGCGGGTTAAGGTCATGTCGCGCATGGACATTGCCGAACGTCGCGTCCCCCAAGACGGGCGTATCAAACTGTTATTGTC
AAAAACCAGGGCCATTGACTTTCGGGTCAATACCTGCCCGACTTTGTTTGGCGAAAAAGTGGTATTGCGTATTCTCGACC
CCACTGCCGCGCAACTGGGCATTGAAAAACTGGGTTTTGAGCCTGAGCAGCAACAACTGTTCCTCAAAGCCATCCATAAA
CCTTATGGCTTGGTACTGGTAACTGGCCCTACGGGTAGCGGCAAAACGGTATCGCTGTACACAGGGCTAAATATCCTCAA
CAGCATCGACCGTAACATTTCCACCGCCGAAGACCCGGTGGAAATTACCGTCGAAGGCATTAACCAGGTCAACGTCAACA
ACAAGGCCGGGCTGACTTTTGCCGCCGCGCTGAAAGCCTTTTTACGGCAAGACCCTGATGTTATTATGGTCGGTGAGATC
CGCGATTTGGAAACCGCCGATATTGCCGTCAAAGCCGCCCAAACCGGCCATTTGGTATTGTCCACCCTACATACCAACGA
CGCGCCGCAAACGCTCAACCGGCTAATGCAAATGGGGGTAGCCCCTTTTAACATCGTCTCGGCGGTCAACCTGATCATGG
CGCAACGGCTGGCCCGCCGCCTCTGCGAACGTTGTAAAGCCCCTATTAAATTTCCCGACAAACTGTTATTGGAGGCGGGT
TTTACCGAAGAGGAACTGCCTGACTTGCAACTGTTCGCCCCAGTCGGTTGTGATAACTGCACTAATGGCTATAAAGGCCG
GGTGGGCATTTATCAGGTTATGACCTTAAGCGAAAAAATGTGCGAATTGATTTTGGAAGGCGGCAACTCCTTACAACTGA
CCCAACTGGCGAAAGCCGAGGGCATTAACGACTTGCGTGCTTCTGGACTGAATAAAGTCCGTATGGGCGTAACTAGCCTA
CAAGAAATTGACCGAGTAACCAAGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.245

100

0.555
  pilB Acinetobacter baumannii D1279779

54.087

100

0.547
  pilB Legionella pneumophila strain ERS1305867

54.021

100

0.543
  pilF Neisseria gonorrhoeae MS11

49.281

97.715

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

47.95

98.594

0.473
  pilB Vibrio cholerae strain A1552

47.95

98.594

0.473
  pilB Vibrio campbellii strain DS40M4

47.34

99.121

0.469
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.961

100

0.385
  pilF Thermus thermophilus HB27

37.05

97.715

0.362

Multiple sequence alignment