Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   CCR98_RS16495 Genome accession   NZ_CP021768
Coordinates   3517212..3517628 (-) Length   138 a.a.
NCBI ID   WP_087923448.1    Uniprot ID   -
Organism   Stenotrophomonas sp. WZN-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3516688..3532659 3517212..3517628 within 0


Gene organization within MGE regions


Location: 3516688..3532659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCR98_RS16490 (CCR98_16495) - 3516688..3517095 (-) 408 WP_087923447.1 pilin -
  CCR98_RS16495 (CCR98_16500) pilA/pilAI 3517212..3517628 (-) 417 WP_087923448.1 pilin Machinery gene
  CCR98_RS16500 (CCR98_16505) pilC 3517984..3519243 (+) 1260 WP_087923449.1 type II secretion system F family protein Machinery gene
  CCR98_RS16505 (CCR98_16510) - 3519251..3520114 (+) 864 WP_005418626.1 A24 family peptidase -
  CCR98_RS16510 (CCR98_16515) coaE 3520126..3520737 (+) 612 WP_087923450.1 dephospho-CoA kinase -
  CCR98_RS21120 - 3520811..3521089 (-) 279 WP_157721541.1 hypothetical protein -
  CCR98_RS21125 - 3521554..3522201 (-) 648 WP_157721542.1 hypothetical protein -
  CCR98_RS16525 (CCR98_16530) - 3523087..3523473 (-) 387 WP_087923453.1 hypothetical protein -
  CCR98_RS16530 (CCR98_16535) - 3523964..3525334 (-) 1371 WP_032957212.1 HAMP domain-containing sensor histidine kinase -
  CCR98_RS16535 (CCR98_16540) - 3525297..3525974 (-) 678 WP_005410804.1 response regulator transcription factor -
  CCR98_RS16540 (CCR98_16545) - 3526136..3526597 (-) 462 WP_087923454.1 hypothetical protein -
  CCR98_RS16545 (CCR98_16550) - 3526713..3527183 (-) 471 WP_087923455.1 hypothetical protein -
  CCR98_RS16550 (CCR98_16555) - 3527279..3527758 (-) 480 WP_087923456.1 hypothetical protein -
  CCR98_RS16555 (CCR98_16560) rimK 3528018..3528896 (-) 879 WP_005410807.1 30S ribosomal protein S6--L-glutamate ligase -
  CCR98_RS16560 (CCR98_16565) - 3529064..3529429 (+) 366 WP_232463040.1 hypothetical protein -
  CCR98_RS16565 (CCR98_16570) - 3529508..3529930 (+) 423 WP_087923458.1 thioesterase family protein -
  CCR98_RS16570 (CCR98_16575) - 3530099..3531133 (-) 1035 WP_232463041.1 hypothetical protein -
  CCR98_RS16575 (CCR98_16580) - 3531375..3532253 (+) 879 WP_232463042.1 PoNe immunity protein domain-containing protein -
  CCR98_RS16580 (CCR98_16585) - 3532255..3532659 (-) 405 WP_087923461.1 DUF805 domain-containing protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14594.01 Da        Isoelectric Point: 6.2914

>NTDB_id=233120 CCR98_RS16495 WP_087923448.1 3517212..3517628(-) (pilA/pilAI) [Stenotrophomonas sp. WZN-1]
MKNQKGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQVTAGLAEITPGKTQYEVALNEGKTTVADIAELGLKDSDRCTI
TPITALSETGSIECTLAGNTKIVGKKIRVERAADGTWACKTDITAAEKKYIPSGCVTG

Nucleotide


Download         Length: 417 bp        

>NTDB_id=233120 CCR98_RS16495 WP_087923448.1 3517212..3517628(-) (pilA/pilAI) [Stenotrophomonas sp. WZN-1]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTCGTTGCGATCATCGCCATCCTGGCCGCCATCGCCCT
GCCGATGTATCAGGACTATGTGGCCAAGTCGCAGGTTACTGCTGGCCTGGCTGAAATCACCCCTGGCAAGACCCAGTACG
AAGTTGCCTTGAACGAAGGCAAGACCACTGTTGCTGATATTGCCGAGCTGGGTCTGAAGGATTCCGATCGCTGCACCATC
ACCCCGATCACTGCGCTGAGCGAGACTGGCTCGATCGAGTGCACCCTTGCGGGCAACACGAAGATCGTCGGCAAGAAGAT
CCGTGTGGAGCGTGCTGCTGATGGCACCTGGGCTTGCAAGACCGATATCACCGCAGCGGAAAAGAAGTACATCCCGAGCG
GCTGCGTCACCGGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

53.623

100

0.536
  pilA Acinetobacter baumannii strain A118

49.65

100

0.514
  pilA Pseudomonas aeruginosa PAK

42.384

100

0.464
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.478

100

0.435
  pilA Vibrio cholerae C6706

40.816

100

0.435
  pilA Vibrio cholerae strain A1552

40.816

100

0.435
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.816

100

0.435
  comP Acinetobacter baylyi ADP1

38.255

100

0.413
  pilE Neisseria gonorrhoeae MS11

33.735

100

0.406
  pilA Vibrio parahaemolyticus RIMD 2210633

39.098

96.377

0.377

Multiple sequence alignment