Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BEI_RS08235 Genome accession   NZ_CP021435
Coordinates   1730651..1731883 (-) Length   410 a.a.
NCBI ID   WP_097789065.1    Uniprot ID   A0A291P713
Organism   Halomonas beimenensis strain NTU-111     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1721818..1751513 1730651..1731883 within 0


Gene organization within MGE regions


Location: 1721818..1751513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEI_RS08190 (BEI_1644) - 1721818..1722111 (+) 294 WP_097789057.1 hypothetical protein -
  BEI_RS08195 (BEI_1645) - 1722348..1723928 (-) 1581 WP_097791037.1 ribonuclease J -
  BEI_RS08200 (BEI_1646) purU 1724233..1725087 (+) 855 WP_097789058.1 formyltetrahydrofolate deformylase -
  BEI_RS08205 (BEI_1647) - 1725124..1725795 (+) 672 WP_097789059.1 energy-coupling factor ABC transporter permease -
  BEI_RS08210 (BEI_1648) - 1725951..1726280 (+) 330 WP_153045777.1 hypothetical protein -
  BEI_RS08215 (BEI_1650) - 1726411..1727582 (+) 1172 WP_097789061.1 IS3 family transposase -
  BEI_RS08220 (BEI_1651) dinB 1727760..1728815 (-) 1056 WP_097789062.1 DNA polymerase IV -
  BEI_RS08225 (BEI_1652) lexA 1728880..1729539 (-) 660 WP_097789063.1 transcriptional repressor LexA -
  BEI_RS08230 (BEI_1653) - 1729774..1730427 (+) 654 WP_097789064.1 TetR/AcrR family transcriptional regulator -
  BEI_RS08235 (BEI_1654) pilC 1730651..1731883 (-) 1233 WP_097789065.1 type II secretion system F family protein Machinery gene
  BEI_RS08240 (BEI_1655) pilB 1732074..1733867 (-) 1794 WP_420809732.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BEI_RS19470 - 1734204..1734315 (+) 112 Protein_1623 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BEI_RS08245 pilA/pilA1 1734306..1734608 (+) 303 WP_417340742.1 pilin Machinery gene
  BEI_RS08250 (BEI_1657) - 1735181..1736869 (-) 1689 WP_097789066.1 PglL family O-oligosaccharyltransferase -
  BEI_RS08255 (BEI_1658) - 1737042..1738457 (+) 1416 WP_097789067.1 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  BEI_RS08260 (BEI_1660) - 1738848..1739120 (-) 273 WP_193765181.1 hypothetical protein -
  BEI_RS08265 (BEI_1661) - 1739322..1741364 (-) 2043 WP_227644648.1 YjbH domain-containing protein -
  BEI_RS08270 (BEI_1662) - 1741406..1742176 (-) 771 WP_097789069.1 capsule biosynthesis GfcC family protein -
  BEI_RS08275 (BEI_1663) - 1742197..1742805 (-) 609 WP_193765182.1 YjbF family lipoprotein -
  BEI_RS19145 (BEI_1664) - 1742919..1743158 (-) 240 WP_193765183.1 hypothetical protein -
  BEI_RS08285 (BEI_1665) - 1743604..1744734 (+) 1131 WP_097789071.1 polysaccharide export protein -
  BEI_RS08290 (BEI_1666) - 1744734..1745171 (+) 438 WP_097789072.1 low molecular weight protein-tyrosine-phosphatase -
  BEI_RS08295 (BEI_1667) - 1745239..1745580 (-) 342 WP_097789073.1 four helix bundle protein -
  BEI_RS08300 (BEI_1668) - 1745681..1747885 (+) 2205 WP_097789074.1 polysaccharide biosynthesis tyrosine autokinase -
  BEI_RS08305 (BEI_1669) - 1748149..1748958 (+) 810 WP_097789075.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  BEI_RS18970 - 1749052..1749291 (-) 240 WP_153045778.1 hypothetical protein -
  BEI_RS08310 (BEI_1671) - 1749353..1750343 (+) 991 Protein_1638 integrase core domain-containing protein -
  BEI_RS08315 (BEI_1673) rfbB 1750464..1751513 (+) 1050 WP_097789076.1 dTDP-glucose 4,6-dehydratase -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44973.75 Da        Isoelectric Point: 9.7521

>NTDB_id=230739 BEI_RS08235 WP_097789065.1 1730651..1731883(-) (pilC) [Halomonas beimenensis strain NTU-111]
MATATMPKSKKIKLYRWRWTGKGPQGRHVSGEIIASKRFEVEKELAGQNIIVKNIRRKSGIGGGMGKIKPRDIMLFARQM
ATMIRAGVPALQAFQVVAESMKKPAMSALVQELMNDVAAGASFSEALKRHPEHFDRLFCNLVEAGEQSGSLDRMLERVAT
YKEKVESLKGRVKKALWYPAAVIAVGIGVTALLLIKVVPQFESLFHGFGAELPAMTRMTIHLSELAQAYWLWVLGGFVAT
VFLIRGGMKRSESFAYRMHALALRIPVIGDILDKSCVARYSRTLATTFGAGVPLVEALETSSGATGNKVYERAVDQVRDD
VATGQQLHFAMRLTEQFPPLAVQMVGIGEEAGALDAMLNRVADYYEEEVDNKVDALTSLLEPFIIVVLGVLVGGLVISMY
LPIFELGSVI

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=230739 BEI_RS08235 WP_097789065.1 1730651..1731883(-) (pilC) [Halomonas beimenensis strain NTU-111]
ATGGCCACCGCAACCATGCCGAAATCCAAGAAGATCAAGCTCTACCGTTGGCGTTGGACCGGCAAGGGACCTCAGGGGCG
CCATGTCAGCGGCGAGATCATCGCCAGCAAGAGGTTTGAGGTCGAGAAGGAGCTGGCCGGCCAGAACATCATCGTCAAGA
ACATTCGCCGCAAGAGCGGCATCGGCGGGGGCATGGGCAAGATCAAGCCCCGCGACATCATGCTGTTCGCGCGTCAGATG
GCCACCATGATCCGCGCCGGGGTGCCCGCCCTGCAGGCCTTCCAGGTCGTGGCGGAGAGCATGAAGAAGCCGGCCATGAG
CGCCCTGGTGCAGGAGCTGATGAACGACGTGGCCGCCGGTGCCAGCTTCTCCGAGGCCCTCAAGCGCCATCCCGAGCACT
TCGATCGGCTGTTCTGCAACCTGGTGGAGGCCGGCGAGCAGTCCGGCTCGCTGGATCGCATGCTCGAGCGGGTGGCCACC
TACAAGGAGAAGGTCGAGTCGCTGAAGGGCCGGGTGAAGAAGGCGCTCTGGTATCCCGCCGCGGTCATCGCCGTGGGCAT
CGGCGTCACCGCCCTGCTGCTGATCAAGGTGGTGCCCCAGTTCGAGAGCCTCTTCCATGGCTTCGGCGCGGAGCTGCCGG
CCATGACGCGCATGACCATCCATCTCTCCGAGCTCGCCCAGGCGTACTGGCTGTGGGTGCTGGGGGGCTTCGTGGCCACC
GTCTTCCTGATCCGCGGCGGGATGAAACGCTCGGAGAGTTTCGCCTATCGGATGCACGCGCTGGCGCTGCGCATCCCGGT
CATCGGCGACATCCTCGACAAGTCCTGCGTGGCGCGCTATTCGCGCACGCTGGCCACCACGTTCGGCGCCGGCGTGCCGC
TGGTGGAGGCCCTGGAGACCTCGTCGGGCGCCACGGGCAACAAGGTCTACGAGCGCGCCGTCGACCAGGTCCGCGACGAC
GTGGCCACCGGCCAGCAGCTGCACTTCGCCATGCGTCTCACCGAGCAGTTCCCGCCGCTGGCGGTGCAGATGGTCGGCAT
CGGCGAGGAAGCCGGCGCCCTGGACGCCATGCTCAACCGCGTGGCGGACTACTACGAGGAAGAGGTCGACAACAAGGTCG
ACGCCCTCACCTCGCTGCTGGAGCCCTTCATCATCGTGGTGCTGGGGGTGCTGGTCGGCGGCCTGGTCATCTCGATGTAC
CTGCCGATCTTCGAGTTGGGGAGTGTGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(281-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A291P713

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.98

98.049

0.529
  pilC Acinetobacter baylyi ADP1

51.75

97.561

0.505
  pilC Acinetobacter baumannii D1279779

51.637

96.829

0.5
  pilC Legionella pneumophila strain ERS1305867

49.875

97.317

0.485
  pilC Vibrio cholerae strain A1552

43.796

100

0.439
  pilC Vibrio campbellii strain DS40M4

44.776

98.049

0.439
  pilG Neisseria gonorrhoeae MS11

43.291

96.341

0.417
  pilG Neisseria meningitidis 44/76-A

42.893

96.098

0.412
  pilC Thermus thermophilus HB27

37.469

98.293

0.368

Multiple sequence alignment