Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA/pilA1   Type   Machinery gene
Locus tag   BEI_RS08245 Genome accession   NZ_CP021435
Coordinates   1734306..1734608 (+) Length   100 a.a.
NCBI ID   WP_417340742.1    Uniprot ID   -
Organism   Halomonas beimenensis strain NTU-111     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1721818..1751513 1734306..1734608 within 0


Gene organization within MGE regions


Location: 1721818..1751513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEI_RS08190 (BEI_1644) - 1721818..1722111 (+) 294 WP_097789057.1 hypothetical protein -
  BEI_RS08195 (BEI_1645) - 1722348..1723928 (-) 1581 WP_097791037.1 ribonuclease J -
  BEI_RS08200 (BEI_1646) purU 1724233..1725087 (+) 855 WP_097789058.1 formyltetrahydrofolate deformylase -
  BEI_RS08205 (BEI_1647) - 1725124..1725795 (+) 672 WP_097789059.1 energy-coupling factor ABC transporter permease -
  BEI_RS08210 (BEI_1648) - 1725951..1726280 (+) 330 WP_153045777.1 hypothetical protein -
  BEI_RS08215 (BEI_1650) - 1726411..1727582 (+) 1172 WP_097789061.1 IS3 family transposase -
  BEI_RS08220 (BEI_1651) dinB 1727760..1728815 (-) 1056 WP_097789062.1 DNA polymerase IV -
  BEI_RS08225 (BEI_1652) lexA 1728880..1729539 (-) 660 WP_097789063.1 transcriptional repressor LexA -
  BEI_RS08230 (BEI_1653) - 1729774..1730427 (+) 654 WP_097789064.1 TetR/AcrR family transcriptional regulator -
  BEI_RS08235 (BEI_1654) pilC 1730651..1731883 (-) 1233 WP_097789065.1 type II secretion system F family protein Machinery gene
  BEI_RS08240 (BEI_1655) pilB 1732074..1733867 (-) 1794 WP_420809732.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BEI_RS19470 - 1734204..1734315 (+) 112 Protein_1623 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BEI_RS08245 pilA/pilA1 1734306..1734608 (+) 303 WP_417340742.1 pilin Machinery gene
  BEI_RS08250 (BEI_1657) - 1735181..1736869 (-) 1689 WP_097789066.1 PglL family O-oligosaccharyltransferase -
  BEI_RS08255 (BEI_1658) - 1737042..1738457 (+) 1416 WP_097789067.1 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  BEI_RS08260 (BEI_1660) - 1738848..1739120 (-) 273 WP_193765181.1 hypothetical protein -
  BEI_RS08265 (BEI_1661) - 1739322..1741364 (-) 2043 WP_227644648.1 YjbH domain-containing protein -
  BEI_RS08270 (BEI_1662) - 1741406..1742176 (-) 771 WP_097789069.1 capsule biosynthesis GfcC family protein -
  BEI_RS08275 (BEI_1663) - 1742197..1742805 (-) 609 WP_193765182.1 YjbF family lipoprotein -
  BEI_RS19145 (BEI_1664) - 1742919..1743158 (-) 240 WP_193765183.1 hypothetical protein -
  BEI_RS08285 (BEI_1665) - 1743604..1744734 (+) 1131 WP_097789071.1 polysaccharide export protein -
  BEI_RS08290 (BEI_1666) - 1744734..1745171 (+) 438 WP_097789072.1 low molecular weight protein-tyrosine-phosphatase -
  BEI_RS08295 (BEI_1667) - 1745239..1745580 (-) 342 WP_097789073.1 four helix bundle protein -
  BEI_RS08300 (BEI_1668) - 1745681..1747885 (+) 2205 WP_097789074.1 polysaccharide biosynthesis tyrosine autokinase -
  BEI_RS08305 (BEI_1669) - 1748149..1748958 (+) 810 WP_097789075.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  BEI_RS18970 - 1749052..1749291 (-) 240 WP_153045778.1 hypothetical protein -
  BEI_RS08310 (BEI_1671) - 1749353..1750343 (+) 991 Protein_1638 integrase core domain-containing protein -
  BEI_RS08315 (BEI_1673) rfbB 1750464..1751513 (+) 1050 WP_097789076.1 dTDP-glucose 4,6-dehydratase -

Sequence


Protein


Download         Length: 100 a.a.        Molecular weight: 10048.14 Da        Isoelectric Point: 3.8342

>NTDB_id=230741 BEI_RS08245 WP_417340742.1 1734306..1734608(+) (pilA/pilA1) [Halomonas beimenensis strain NTU-111]
MGRAQASEGLSVVSGVQLDVAEQVAQDGALTTNISLSPTLDGKYISGVTVASNGVITVSFDAGVHSGQTMTLTPTEANGQ
ITGWTCAGLADTAYLPSSCQ

Nucleotide


Download         Length: 303 bp        

>NTDB_id=230741 BEI_RS08245 WP_417340742.1 1734306..1734608(+) (pilA/pilA1) [Halomonas beimenensis strain NTU-111]
GTGGGTAGGGCGCAGGCCTCTGAAGGCTTGTCCGTAGTCTCTGGGGTGCAATTAGATGTTGCGGAGCAGGTTGCTCAAGA
CGGAGCATTGACTACTAATATTTCATTGTCCCCTACGCTGGATGGGAAATATATTAGCGGGGTGACAGTTGCATCGAATG
GTGTTATTACGGTAAGTTTTGACGCTGGTGTTCACAGTGGTCAAACCATGACATTGACACCCACGGAAGCAAATGGTCAA
ATTACTGGTTGGACGTGTGCCGGCTTAGCTGATACGGCCTACTTGCCAAGTTCATGCCAGTAA

Domains


Predicted by InterproScan.

(3-100)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

42.241

100

0.49

  pilA2 Legionella pneumophila str. Paris

34.906

100

0.37

  pilA2 Legionella pneumophila strain ERS1305867

34.906

100

0.37

  comP Acinetobacter baylyi ADP1

32.174

100

0.37


Multiple sequence alignment