Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BEI_RS08240 Genome accession   NZ_CP021435
Coordinates   1732074..1733867 (-) Length   597 a.a.
NCBI ID   WP_420809732.1    Uniprot ID   -
Organism   Halomonas beimenensis strain NTU-111     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1721818..1751513 1732074..1733867 within 0


Gene organization within MGE regions


Location: 1721818..1751513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEI_RS08190 (BEI_1644) - 1721818..1722111 (+) 294 WP_097789057.1 hypothetical protein -
  BEI_RS08195 (BEI_1645) - 1722348..1723928 (-) 1581 WP_097791037.1 ribonuclease J -
  BEI_RS08200 (BEI_1646) purU 1724233..1725087 (+) 855 WP_097789058.1 formyltetrahydrofolate deformylase -
  BEI_RS08205 (BEI_1647) - 1725124..1725795 (+) 672 WP_097789059.1 energy-coupling factor ABC transporter permease -
  BEI_RS08210 (BEI_1648) - 1725951..1726280 (+) 330 WP_153045777.1 hypothetical protein -
  BEI_RS08215 (BEI_1650) - 1726411..1727582 (+) 1172 WP_097789061.1 IS3 family transposase -
  BEI_RS08220 (BEI_1651) dinB 1727760..1728815 (-) 1056 WP_097789062.1 DNA polymerase IV -
  BEI_RS08225 (BEI_1652) lexA 1728880..1729539 (-) 660 WP_097789063.1 transcriptional repressor LexA -
  BEI_RS08230 (BEI_1653) - 1729774..1730427 (+) 654 WP_097789064.1 TetR/AcrR family transcriptional regulator -
  BEI_RS08235 (BEI_1654) pilC 1730651..1731883 (-) 1233 WP_097789065.1 type II secretion system F family protein Machinery gene
  BEI_RS08240 (BEI_1655) pilB 1732074..1733867 (-) 1794 WP_420809732.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BEI_RS19470 - 1734204..1734315 (+) 112 Protein_1623 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BEI_RS08245 pilA/pilA1 1734306..1734608 (+) 303 WP_417340742.1 pilin Machinery gene
  BEI_RS08250 (BEI_1657) - 1735181..1736869 (-) 1689 WP_097789066.1 PglL family O-oligosaccharyltransferase -
  BEI_RS08255 (BEI_1658) - 1737042..1738457 (+) 1416 WP_097789067.1 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  BEI_RS08260 (BEI_1660) - 1738848..1739120 (-) 273 WP_193765181.1 hypothetical protein -
  BEI_RS08265 (BEI_1661) - 1739322..1741364 (-) 2043 WP_227644648.1 YjbH domain-containing protein -
  BEI_RS08270 (BEI_1662) - 1741406..1742176 (-) 771 WP_097789069.1 capsule biosynthesis GfcC family protein -
  BEI_RS08275 (BEI_1663) - 1742197..1742805 (-) 609 WP_193765182.1 YjbF family lipoprotein -
  BEI_RS19145 (BEI_1664) - 1742919..1743158 (-) 240 WP_193765183.1 hypothetical protein -
  BEI_RS08285 (BEI_1665) - 1743604..1744734 (+) 1131 WP_097789071.1 polysaccharide export protein -
  BEI_RS08290 (BEI_1666) - 1744734..1745171 (+) 438 WP_097789072.1 low molecular weight protein-tyrosine-phosphatase -
  BEI_RS08295 (BEI_1667) - 1745239..1745580 (-) 342 WP_097789073.1 four helix bundle protein -
  BEI_RS08300 (BEI_1668) - 1745681..1747885 (+) 2205 WP_097789074.1 polysaccharide biosynthesis tyrosine autokinase -
  BEI_RS08305 (BEI_1669) - 1748149..1748958 (+) 810 WP_097789075.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  BEI_RS18970 - 1749052..1749291 (-) 240 WP_153045778.1 hypothetical protein -
  BEI_RS08310 (BEI_1671) - 1749353..1750343 (+) 991 Protein_1638 integrase core domain-containing protein -
  BEI_RS08315 (BEI_1673) rfbB 1750464..1751513 (+) 1050 WP_097789076.1 dTDP-glucose 4,6-dehydratase -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 65181.52 Da        Isoelectric Point: 5.0163

>NTDB_id=230740 BEI_RS08240 WP_420809732.1 1732074..1733867(-) (pilB) [Halomonas beimenensis strain NTU-111]
MNDYRPPGPEQVETPQPQDSPLSSVANRRGMRGLSKRLVADGLISEAAASRAEEVAREAEVSLLEHIIDAGMVPARQATL
TAAWEYGLPIIDLDALRLASLPPAAEFPDKILRRLGILPLTRHGHRLTVAVPYPSTLAQLDELQFATGLTPEGVLAPVDQ
LGPVLESYLASTENSMLDELANVDDAIGELEYDEGATDITDEQQQVAAAGDDAPIVKFVNKILLDAIRRGASDIHFEPYE
TSYRVRFRIDGMLHDVAHPPFAMRTRIAARLKVMSRLDISERRLPQDGAIKLRISKTRSIDFRVNSLPTVYGEKVVLRIL
DPSSAQLGIEELGFTPEQRAMYEAVLDDPQGMILVTGPTGSGKTVTLYTGINILNEVERNICTAEDPVEIKVPGVNQVNV
LPKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVAPFNIASSVSLI
IAQRLARRLCKHCKQPADIPREALLSQGFTPEEVEQATLYEPVGCKHCTQGFKGRVGIYEVVPVSDAMSKLIMTHGNSMD
FDAQARQEGHPDLRRSGLLKVMQGLTSLAEVNRVTKD

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=230740 BEI_RS08240 WP_420809732.1 1732074..1733867(-) (pilB) [Halomonas beimenensis strain NTU-111]
ATGAACGACTACCGCCCCCCCGGCCCCGAACAGGTCGAGACGCCCCAGCCCCAGGATTCGCCCCTTTCCAGCGTGGCGAA
CCGACGCGGCATGCGCGGGCTCTCCAAGCGGCTGGTCGCCGACGGGCTGATCAGCGAGGCCGCGGCCAGCCGCGCCGAGG
AAGTGGCCCGGGAGGCCGAGGTCTCGCTGCTGGAGCACATCATCGACGCCGGCATGGTGCCCGCCCGCCAGGCCACCCTG
ACCGCCGCCTGGGAATACGGGCTGCCGATCATCGACCTGGATGCCCTGCGCCTGGCGAGCCTGCCCCCGGCGGCGGAGTT
TCCGGACAAGATCCTGCGCCGGCTGGGCATCCTGCCGCTCACCCGGCACGGGCACCGCCTCACGGTGGCCGTGCCCTACC
CGTCCACCCTGGCCCAGCTCGACGAGCTGCAGTTCGCCACCGGCCTGACCCCGGAAGGCGTCCTGGCGCCGGTCGACCAA
CTGGGCCCGGTGCTGGAGAGCTACCTGGCGAGCACCGAGAACTCGATGCTCGACGAGCTGGCCAACGTCGACGATGCCAT
CGGCGAGCTGGAGTACGACGAAGGCGCCACCGACATCACCGACGAACAGCAGCAGGTGGCGGCCGCCGGCGACGACGCCC
CCATCGTCAAGTTCGTCAACAAGATCCTGCTGGACGCCATCCGGCGCGGCGCCTCGGATATTCACTTCGAGCCCTACGAG
ACCAGCTACCGGGTACGCTTCCGCATCGACGGCATGCTGCACGACGTGGCGCATCCGCCCTTCGCCATGCGCACGCGGAT
CGCGGCCCGGCTCAAGGTGATGTCGCGGCTGGACATCTCCGAGCGCCGGCTGCCCCAGGACGGCGCCATCAAGCTGCGGA
TCTCCAAGACCCGTTCGATCGACTTCCGCGTCAACTCCCTGCCCACCGTCTACGGCGAGAAGGTCGTGCTGCGGATCCTC
GACCCGTCCTCGGCGCAGCTGGGCATCGAGGAACTGGGCTTCACCCCCGAGCAGCGCGCCATGTACGAGGCGGTGCTGGA
CGATCCCCAGGGCATGATCCTGGTCACCGGCCCCACCGGCAGCGGCAAGACGGTGACCCTCTACACGGGTATCAACATCC
TCAACGAGGTGGAGCGCAACATCTGCACCGCCGAGGACCCGGTGGAGATCAAGGTGCCCGGGGTCAACCAGGTGAACGTG
CTGCCCAAGATCGGCCTCGACTTCGCCAGCGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGGTGATGGTCGGCGA
GATCCGTGACCTGGAGACCGCCGAGATCGCGGTCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTCCACGGTACACACCA
ACTCGGCGGCGGAAACGCTCACGCGCCTGGCCAACATGGGCGTCGCGCCCTTCAATATCGCCAGTTCCGTGAGCCTGATC
ATCGCCCAGCGCCTCGCCCGCCGCCTGTGCAAGCACTGCAAGCAGCCCGCCGATATCCCCCGGGAGGCGCTGCTCTCCCA
GGGCTTCACCCCGGAGGAGGTCGAGCAGGCCACCCTCTACGAACCGGTGGGCTGCAAGCACTGTACCCAGGGCTTCAAGG
GTCGCGTCGGCATCTACGAGGTGGTGCCCGTCTCCGACGCCATGAGCAAGCTGATCATGACCCACGGCAACTCCATGGAC
TTCGATGCCCAGGCCCGGCAGGAGGGGCACCCGGACCTGCGCCGCAGCGGCCTGCTCAAGGTCATGCAGGGCCTGACCAG
CCTGGCGGAGGTCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-172)

Type II/IV secretion system protein (T2SSE)

(221-488)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.59

95.645

0.513
  pilB Acinetobacter baumannii D1279779

52.504

96.985

0.509
  pilB Acinetobacter baylyi ADP1

52.797

95.812

0.506
  pilB Vibrio cholerae strain A1552

51.124

89.447

0.457
  pilB Vibrio campbellii strain DS40M4

47.535

95.142

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

47.193

95.477

0.451
  pilF Neisseria gonorrhoeae MS11

50.826

81.072

0.412

Multiple sequence alignment