Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CAY59_RS02475 Genome accession   NZ_CP021145
Coordinates   497973..499721 (+) Length   582 a.a.
NCBI ID   WP_045369316.1    Uniprot ID   A0AAQ2Y0E1
Organism   Vibrio campbellii strain LA16-V1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 492973..504721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAY59_RS02455 (CAY59_02415) pilM 495272..496288 (+) 1017 WP_005432712.1 type IV pilus assembly protein PilM Machinery gene
  CAY59_RS02460 (CAY59_02420) pilN 496272..496847 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  CAY59_RS02465 (CAY59_02425) pilO 496840..497433 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  CAY59_RS02470 (CAY59_02430) pilP 497423..497938 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  CAY59_RS02475 (CAY59_02435) pilQ 497973..499721 (+) 1749 WP_045369316.1 type IV pilus secretin PilQ Machinery gene
  CAY59_RS02480 (CAY59_02440) aroK 499910..500428 (+) 519 WP_010443904.1 shikimate kinase AroK -
  CAY59_RS02485 (CAY59_02445) aroB 500460..501560 (+) 1101 WP_086368939.1 3-dehydroquinate synthase -
  CAY59_RS02490 (CAY59_02450) - 501614..503107 (+) 1494 WP_086368940.1 AAA family ATPase -
  CAY59_RS02495 (CAY59_02455) - 503177..504016 (+) 840 WP_038889578.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63787.73 Da        Isoelectric Point: 4.5130

>NTDB_id=228555 CAY59_RS02475 WP_045369316.1 497973..499721(+) (pilQ) [Vibrio campbellii strain LA16-V1]
MKMRHGLTLTPRKLTGLFSLWFFLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGDDGGSSVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=228555 CAY59_RS02475 WP_045369316.1 497973..499721(+) (pilQ) [Vibrio campbellii strain LA16-V1]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTTTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATCGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTATAACCAATTTAACTTGGTCGTCTC
TGACTCGGTTGAGGGTAACTTGACCTTGCGTTTGGATGGTGTGCCTTGGCAGCAAGTACTCGACATTATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTTGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGGGAGGGCAACATCAGTATGCTTTCGGATCGAGGCAGCATGACCATTGATGAGCGAACAA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCACGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACTTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCCTGTACGATGGCGGCGGTGATG
GTGAAGGTGATGACGGTGGCAGCAGTGTGGGTATTGACGACTTTCTCAACGTTAACCTTGCTGCGACCAGTCCAAATGCG
ACCAGTATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATC
GAAAGCAGAAATTATTTCCAGTCCACGTTTGATCACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCC
CTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACGCCACAG
ATTACGCCTGACAACCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGG
TGAGGCGGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTTAATAATGGCGAGACAGTCGTGTTGGGCGGTA
TTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCGT
CGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

99.141

100

0.991
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.465

97.595

0.756
  pilQ Vibrio cholerae strain A1552

77.465

97.595

0.756
  pilQ Legionella pneumophila strain ERS1305867

41.742

94.674

0.395
  pilQ Legionella pneumophila strain Lp02

41.742

94.674

0.395
  pilQ Pseudomonas aeruginosa PAK

40.32

96.735

0.39
  pilQ Acinetobacter baumannii D1279779

37.271

100

0.385
  pilQ Acinetobacter baumannii strain A118

37.271

100

0.385
  comQ Acinetobacter baylyi ADP1

37.005

99.828

0.369

Multiple sequence alignment