Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CAY59_RS02455 Genome accession   NZ_CP021145
Coordinates   495272..496288 (+) Length   338 a.a.
NCBI ID   WP_005432712.1    Uniprot ID   -
Organism   Vibrio campbellii strain LA16-V1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490272..501288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAY59_RS02440 (CAY59_02400) - 490637..491365 (-) 729 WP_077200153.1 redoxin family protein -
  CAY59_RS02445 (CAY59_02405) oxyR 491540..492448 (+) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  CAY59_RS02450 (CAY59_02410) - 492526..495093 (-) 2568 WP_038889585.1 penicillin-binding protein 1A -
  CAY59_RS02455 (CAY59_02415) pilM 495272..496288 (+) 1017 WP_005432712.1 type IV pilus assembly protein PilM Machinery gene
  CAY59_RS02460 (CAY59_02420) pilN 496272..496847 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  CAY59_RS02465 (CAY59_02425) pilO 496840..497433 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  CAY59_RS02470 (CAY59_02430) pilP 497423..497938 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  CAY59_RS02475 (CAY59_02435) pilQ 497973..499721 (+) 1749 WP_045369316.1 type IV pilus secretin PilQ Machinery gene
  CAY59_RS02480 (CAY59_02440) aroK 499910..500428 (+) 519 WP_010443904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37860.29 Da        Isoelectric Point: 6.3141

>NTDB_id=228551 CAY59_RS02455 WP_005432712.1 495272..496288(+) (pilM) [Vibrio campbellii strain LA16-V1]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=228551 CAY59_RS02455 WP_005432712.1 495272..496288(+) (pilM) [Vibrio campbellii strain LA16-V1]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAGTCTCCATTCCCTGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACTGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAAGATATTAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

98.225

100

0.982
  pilM Vibrio cholerae strain A1552

49.415

100

0.5

Multiple sequence alignment