Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   HV91_RS25415 Genome accession   NZ_CP026680
Coordinates   5263591..5264055 (-) Length   154 a.a.
NCBI ID   WP_104879006.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F5677     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5258591..5269055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV91_RS25390 (HV91_25390) nadC 5259975..5260823 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HV91_RS25400 (HV91_25400) - 5261004..5262161 (-) 1158 WP_225321793.1 O-antigen ligase family protein -
  HV91_RS25405 (HV91_25405) - 5262227..5263255 (+) 1029 WP_034023821.1 IS30-like element ISPsp7 family transposase -
  HV91_RS25415 (HV91_25415) pilA/pilAI 5263591..5264055 (-) 465 WP_104879006.1 pilin Machinery gene
  HV91_RS25420 (HV91_25420) pilB 5264286..5265986 (+) 1701 WP_030048379.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HV91_RS25425 (HV91_25425) pilC 5265990..5267207 (+) 1218 WP_015648061.1 type II secretion system F family protein Machinery gene
  HV91_RS25430 (HV91_25430) pilD 5267208..5268080 (+) 873 WP_015648060.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HV91_RS25435 (HV91_25435) coaE 5268077..5268688 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HV91_RS25440 (HV91_25440) yacG 5268685..5268885 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16206.51 Da        Isoelectric Point: 8.4551

>NTDB_id=228097 HV91_RS25415 WP_104879006.1 5263591..5264055(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F5677]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGRSSGKGIADAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=228097 HV91_RS25415 WP_104879006.1 5263591..5264055(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F5677]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGACGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.671

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Ralstonia pseudosolanacearum GMI1000

36.471

100

0.403
  pilA Pseudomonas aeruginosa PAK

38.854

100

0.396
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364
  pilA2 Legionella pneumophila strain ERS1305867

37.838

96.104

0.364