Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   B2J77_RS19770 Genome accession   NZ_CP019952
Coordinates   4387460..4388725 (-) Length   421 a.a.
NCBI ID   WP_058638117.1    Uniprot ID   A0AAJ0LL74
Organism   Pseudomonas parafulva strain PRS09-11288     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4382460..4393725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2J77_RS19755 (B2J77_19970) - 4384132..4385724 (-) 1593 WP_058638115.1 SPOR domain-containing protein -
  B2J77_RS19760 (B2J77_19975) aroB 4385740..4386837 (-) 1098 WP_058602920.1 3-dehydroquinate synthase -
  B2J77_RS19765 (B2J77_19980) aroK 4386937..4387455 (-) 519 WP_058638116.1 shikimate kinase AroK -
  B2J77_RS19770 (B2J77_19985) pilQ 4387460..4388725 (-) 1266 WP_058638117.1 type IV pilus secretin PilQ Machinery gene
  B2J77_RS19775 (B2J77_19990) - 4388649..4389665 (-) 1017 WP_078479272.1 pilus assembly protein PilP -
  B2J77_RS19780 (B2J77_19995) - 4389662..4390198 (-) 537 WP_078479273.1 PilN domain-containing protein -
  B2J77_RS19785 (B2J77_20000) pilM 4390195..4391076 (-) 882 WP_078479274.1 type IV pilus biogenesis protein PilM -
  B2J77_RS19790 (B2J77_20005) - 4391251..4393701 (+) 2451 WP_078479275.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46174.76 Da        Isoelectric Point: 4.9165

>NTDB_id=219128 B2J77_RS19770 WP_058638117.1 4387460..4388725(-) (pilQ) [Pseudomonas parafulva strain PRS09-11288]
MSKTWKWLLATLLLPLTAMAAPYQGERLSLNFQDVEVRAVLQVLADHAGINLVASDDVQGSVTLRLEDVPWDQAIDLVLR
SKGLGRRQEGNVLLIAPLSTLNAQSFEGTANSLDVQQQPMRRELMRIHYAKAADLSELLLASLSEDGILAGRGSLGVDER
TNTLVVHQPANRLAEIRQLIAQLDVPVRQVAIEARIVEANVDYQKGLGVRWGGDLYSNELRPGKDLFVDLGVERGGSSLG
VGLLRGDVLLDLELSAMEKSGEGEIISQPKVVTADKETARILKGTEVPYQQSNKNGATTVSFREASLSLEVTPQITPDDS
VVMTVRVTKDEPDYVNALNDVPPIRKNEVNAKVRVENGQTIVIGGVYSTTQNNVVDKVPFLGDLPYVGRLFRRDVLQEKK
SELLVFLTPRIMSDQTIAVSR

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=219128 B2J77_RS19770 WP_058638117.1 4387460..4388725(-) (pilQ) [Pseudomonas parafulva strain PRS09-11288]
ATGAGCAAGACATGGAAATGGCTACTCGCAACCCTGCTGTTGCCGTTGACGGCAATGGCCGCCCCCTACCAGGGTGAACG
GCTGTCACTGAATTTTCAGGATGTCGAGGTACGCGCCGTGCTGCAGGTGCTTGCCGACCATGCCGGTATCAACCTGGTCG
CCAGCGACGACGTCCAAGGGAGCGTCACCTTGCGGCTGGAAGATGTCCCCTGGGATCAAGCGATCGACCTGGTATTGCGC
AGCAAGGGCCTGGGTCGACGCCAGGAAGGCAATGTACTGCTGATCGCACCGCTTTCGACCCTGAACGCGCAGTCGTTCGA
GGGCACCGCAAATTCACTCGACGTGCAACAACAGCCCATGCGTCGAGAACTGATGCGTATCCACTATGCCAAGGCCGCAG
ATTTGTCCGAGCTGTTGTTGGCGAGCCTGTCTGAGGACGGCATCCTTGCCGGCAGGGGCAGCCTGGGTGTGGATGAGCGT
ACCAACACGCTGGTTGTCCACCAGCCGGCGAATCGACTTGCCGAGATTCGGCAACTGATCGCCCAGTTGGATGTGCCCGT
GCGTCAGGTCGCGATCGAAGCCCGTATCGTGGAGGCCAATGTCGACTACCAGAAAGGCCTGGGGGTGCGATGGGGCGGCG
ACCTGTACAGCAACGAACTAAGACCGGGCAAGGACCTGTTTGTAGACCTGGGCGTCGAGCGGGGCGGCAGCAGCCTTGGC
GTTGGTCTGTTGCGCGGCGACGTGCTCCTTGATCTTGAGCTCAGCGCCATGGAAAAGAGCGGTGAGGGGGAGATCATCTC
GCAGCCCAAGGTAGTGACCGCTGACAAGGAGACGGCGCGGATACTCAAAGGTACCGAGGTGCCTTACCAGCAGTCCAACA
AGAACGGCGCGACGACTGTGTCGTTTCGCGAGGCTTCGCTTTCACTGGAAGTAACGCCGCAGATCACGCCCGATGACAGT
GTCGTCATGACCGTGAGAGTGACCAAGGACGAACCTGACTATGTCAATGCCCTGAATGACGTCCCGCCCATTCGCAAGAA
CGAGGTCAATGCCAAGGTCAGGGTCGAGAACGGACAGACGATCGTGATCGGAGGCGTCTACTCGACCACGCAAAACAATG
TGGTGGACAAGGTGCCATTTCTTGGCGATCTGCCGTATGTTGGACGGCTGTTTCGACGCGATGTACTACAAGAGAAAAAA
TCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGACGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-189)

Bacterial type II and III secretion system protein (Secretin)

(256-412)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

55

100

0.575
  pilQ Acinetobacter baumannii strain A118

42.358

100

0.461
  pilQ Acinetobacter baumannii D1279779

42.358

100

0.461
  comQ Acinetobacter baylyi ADP1

42.572

100

0.456
  pilQ Legionella pneumophila strain ERS1305867

43.301

99.287

0.43
  pilQ Legionella pneumophila strain Lp02

42.823

99.287

0.425
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.106

98.812

0.406
  pilQ Vibrio cholerae strain A1552

41.106

98.812

0.406
  pilQ Vibrio campbellii strain DS40M4

39.906

100

0.404

Multiple sequence alignment