Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC54_RS13595 Genome accession   NZ_CP019121
Coordinates   2696470..2698158 (+) Length   562 a.a.
NCBI ID   WP_101958937.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_054     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2691470..2703158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC54_RS13575 (FORC54_2462) pdhR 2693109..2693876 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FORC54_RS13580 (FORC54_2464) ampD 2694265..2694837 (-) 573 WP_080526792.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC54_RS13585 (FORC54_2465) nadC 2694930..2695817 (+) 888 WP_080526793.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC54_RS13590 (FORC54_2466) pilA 2696040..2696465 (+) 426 WP_038964707.1 pilin Machinery gene
  FORC54_RS13595 (FORC54_2467) pilB 2696470..2698158 (+) 1689 WP_101958937.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC54_RS13600 (FORC54_2468) pilC 2698206..2699432 (+) 1227 WP_101958938.1 type II secretion system F family protein Machinery gene
  FORC54_RS13605 (FORC54_2469) pilD 2699525..2700394 (+) 870 WP_101958939.1 prepilin peptidase Machinery gene
  FORC54_RS13610 (FORC54_2470) coaE 2700396..2701004 (+) 609 WP_045593985.1 dephospho-CoA kinase -
  FORC54_RS13615 (FORC54_2471) zapD 2701037..2701777 (+) 741 WP_017421189.1 cell division protein ZapD -
  FORC54_RS13620 yacG 2701840..2702034 (+) 195 WP_038941426.1 DNA gyrase inhibitor YacG -
  FORC54_RS13625 (FORC54_2472) rplS 2702258..2702611 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62387.45 Da        Isoelectric Point: 5.8436

>NTDB_id=213334 FORC54_RS13595 WP_101958937.1 2696470..2698158(+) (pilB) [Vibrio vulnificus strain FORC_054]
MQSNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEKLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQDLQAAIRRLYGKSIKQSQGSGLKEIHQDELA
TLVEVSDDEIESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFNEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=213334 FORC54_RS13595 WP_101958937.1 2696470..2698158(+) (pilB) [Vibrio vulnificus strain FORC_054]
GTGCAATCCAACCTTGCCACGATTCTGCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCTGATGAACTAACCGAAAAGCTAA
GTGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTAGCCGATTGTCAAGATCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
ACTTTGGTCGAGGTTTCTGATGACGAAATCGAGTCCATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGTTTTAT
CAACCAAATTCTGCTTGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTAC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAA
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGCATTAAGCTACGCCTTAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACTTTACCTACGCTGTTTGGTGAAAAAATTGTTCTGCGACTTCTCGACAGCTCGAGTAGCA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCTCTGTACACGGGCTTGAGTTTGCTTAACCGACCTGAAGT
GAATATCTCCACCGCGGAAGACCCGGTCGAGATTAACCTCCCAGGTATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCCGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTACATACAAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACCTGGCTTCATCCTTAAGTTTGATCATTGCTCAGCGTCTTG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCGCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCCGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTAATGAGCAGCTAGCAGAGGCGATCGTGAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTACAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.335

100

0.763
  pilB Vibrio parahaemolyticus RIMD 2210633

75.801

100

0.758
  pilB Vibrio cholerae strain A1552

74.245

100

0.744
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

50.76

93.594

0.475
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

39.856

99.11

0.395
  pilB/pilB1 Synechocystis sp. PCC 6803

37.063

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363
  pilB Haemophilus influenzae Rd KW20

41.942

86.121

0.361

Multiple sequence alignment