Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BUQ72_RS14085 Genome accession   NZ_CP018845
Coordinates   3175248..3176450 (+) Length   400 a.a.
NCBI ID   WP_079217093.1    Uniprot ID   -
Organism   Herbaspirillum robiniae strain AA6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3170248..3181450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUQ72_RS14065 - 3170524..3171612 (-) 1089 WP_079217089.1 ABC transporter ATP-binding protein -
  BUQ72_RS14070 - 3171835..3172689 (+) 855 WP_079217090.1 IclR family transcriptional regulator -
  BUQ72_RS14075 - 3172686..3173645 (+) 960 WP_079217091.1 2-hydroxyacid dehydrogenase -
  BUQ72_RS14080 - 3173789..3175099 (+) 1311 WP_079217092.1 HlyC/CorC family transporter -
  BUQ72_RS14085 pilF 3175248..3176450 (+) 1203 WP_079217093.1 GspE/PulE family protein Machinery gene
  BUQ72_RS14090 - 3176437..3177720 (+) 1284 WP_079217094.1 type II secretion system F family protein -
  BUQ72_RS14095 pilD 3177737..3178600 (+) 864 WP_079217095.1 prepilin peptidase Machinery gene
  BUQ72_RS14100 coaE 3178613..3179233 (+) 621 WP_079217096.1 dephospho-CoA kinase -
  BUQ72_RS14105 zapD 3179466..3180221 (+) 756 WP_079217097.1 cell division protein ZapD -
  BUQ72_RS14110 yacG 3180231..3180416 (+) 186 WP_079217098.1 DNA gyrase inhibitor YacG -
  BUQ72_RS14115 - 3180718..3181140 (-) 423 WP_079217099.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 43645.38 Da        Isoelectric Point: 7.9144

>NTDB_id=210960 BUQ72_RS14085 WP_079217093.1 3175248..3176450(+) (pilF) [Herbaspirillum robiniae strain AA6]
MDNHSQPAGPAAAHIDDAPVVRLLHKMLADAVGMGASDLHFEPFEHFCRVRFRIDGMLHEMAQPPLTLKDKLSTRLKILS
RLDIAEKRLPQDGKMRLELDGREVDFRVSTLPTQFGEKIVLRILDGAQDSLGIERLGYEPAQHDALMRAIRKPHGMVLMT
GPTGSGKTVSLYACLQLLNRPGVNIATAEDPVEINLPGINQVGINERAGLSFAVALRAFLRQDPDVLMVGEIRDLETADI
AVKASQTGHLVFSTLHTNDAPATLTRLLNMGVPAFNIASSVSLIVAQRLVRRLCDCRHEVRASPDTLKRAGFSEADIDRA
PSLFRPCGCERCNGSGYRGRTGIYQVMPISAAMEGLILAQGGTPDIARQARSEGIADLRRSGLLKTLRGETSLREVIACT

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=210960 BUQ72_RS14085 WP_079217093.1 3175248..3176450(+) (pilF) [Herbaspirillum robiniae strain AA6]
ATGGACAATCACAGCCAGCCCGCCGGCCCGGCCGCCGCCCACATCGACGATGCGCCGGTCGTGCGCCTGCTGCACAAGAT
GCTGGCCGACGCCGTCGGCATGGGCGCATCCGACCTCCACTTCGAACCCTTCGAACACTTCTGCCGCGTGCGCTTTCGCA
TCGACGGCATGCTGCACGAGATGGCGCAGCCGCCGCTGACGCTCAAGGACAAGCTCTCCACCCGGCTCAAGATCCTCTCG
CGCCTGGATATCGCCGAAAAGCGCCTGCCGCAGGACGGCAAGATGCGGCTGGAGCTGGATGGCCGCGAGGTCGATTTCCG
CGTCTCCACGCTGCCCACGCAGTTCGGCGAAAAGATCGTGCTGCGCATTCTCGACGGCGCGCAGGACAGCCTGGGCATAG
AGCGGCTCGGCTACGAGCCGGCCCAGCACGACGCCCTGATGCGCGCCATCCGCAAACCCCACGGCATGGTGCTGATGACC
GGGCCCACCGGGTCGGGCAAGACGGTGTCGCTGTACGCCTGCCTGCAACTGCTCAACCGGCCTGGCGTGAACATCGCCAC
GGCCGAGGATCCGGTGGAGATCAACCTACCGGGCATCAACCAGGTCGGCATCAACGAGCGCGCGGGGCTGTCCTTCGCGG
TGGCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGCTGATGGTGGGCGAGATCCGCGACCTCGAAACCGCCGACATC
GCCGTCAAGGCCTCGCAGACCGGGCACCTGGTGTTTTCCACGCTGCACACCAACGACGCCCCGGCCACGCTCACGCGCCT
GCTCAACATGGGCGTGCCGGCCTTCAACATCGCCTCCTCGGTCAGCCTGATCGTCGCCCAGCGCCTGGTGCGCAGGCTGT
GCGACTGCCGCCACGAGGTGCGCGCCTCGCCCGATACCCTCAAGCGCGCCGGCTTCAGCGAGGCCGACATCGACCGCGCG
CCCAGCCTGTTCCGGCCCTGCGGCTGCGAGCGCTGCAACGGCAGCGGCTACCGCGGGCGCACCGGCATCTACCAAGTCAT
GCCGATCAGCGCGGCGATGGAGGGCCTGATACTGGCGCAAGGCGGCACGCCGGACATCGCCCGGCAGGCGCGCAGCGAGG
GCATCGCCGACCTGCGCCGCTCGGGCCTGCTGAAAACGCTGCGCGGCGAGACCAGCCTGAGGGAAGTCATTGCCTGCACC
TGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(26-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

61.183

97.25

0.595
  pilB Acinetobacter baumannii D1279779

54.975

100

0.552
  pilB Acinetobacter baylyi ADP1

56.218

96.5

0.543
  pilB Legionella pneumophila strain ERS1305867

53.518

99.5

0.533
  pilB Vibrio cholerae strain A1552

54.569

95.75

0.522
  pilB Vibrio parahaemolyticus RIMD 2210633

54.167

96

0.52
  pilB Vibrio campbellii strain DS40M4

53.646

96

0.515
  pilF Thermus thermophilus HB27

46.173

98

0.452
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.14

100

0.443
  pilB/pilB1 Synechocystis sp. PCC 6803

44.361

99.75

0.443
  pilB Haemophilus influenzae 86-028NP

42.82

95.75

0.41
  pilB Haemophilus influenzae Rd KW20

42.408

95.5

0.405
  ctsE Campylobacter jejuni subsp. jejuni 81-176

38.791

99.25

0.385
  pilB Glaesserella parasuis strain SC1401

43.114

83.5

0.36

Multiple sequence alignment