Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BUQ72_RS14095 Genome accession   NZ_CP018845
Coordinates   3177737..3178600 (+) Length   287 a.a.
NCBI ID   WP_079217095.1    Uniprot ID   -
Organism   Herbaspirillum robiniae strain AA6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3172737..3183600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUQ72_RS14080 - 3173789..3175099 (+) 1311 WP_079217092.1 HlyC/CorC family transporter -
  BUQ72_RS14085 pilF 3175248..3176450 (+) 1203 WP_079217093.1 GspE/PulE family protein Machinery gene
  BUQ72_RS14090 - 3176437..3177720 (+) 1284 WP_079217094.1 type II secretion system F family protein -
  BUQ72_RS14095 pilD 3177737..3178600 (+) 864 WP_079217095.1 prepilin peptidase Machinery gene
  BUQ72_RS14100 coaE 3178613..3179233 (+) 621 WP_079217096.1 dephospho-CoA kinase -
  BUQ72_RS14105 zapD 3179466..3180221 (+) 756 WP_079217097.1 cell division protein ZapD -
  BUQ72_RS14110 yacG 3180231..3180416 (+) 186 WP_079217098.1 DNA gyrase inhibitor YacG -
  BUQ72_RS14115 - 3180718..3181140 (-) 423 WP_079217099.1 NUDIX domain-containing protein -
  BUQ72_RS14120 - 3181140..3182015 (-) 876 WP_079219226.1 ATP-binding protein -
  BUQ72_RS14125 argJ 3182332..3183567 (-) 1236 WP_079217100.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 30893.67 Da        Isoelectric Point: 6.7328

>NTDB_id=210961 BUQ72_RS14095 WP_079217095.1 3177737..3178600(+) (pilD) [Herbaspirillum robiniae strain AA6]
MLFLEAYAEPNNAFYSLAAAVLGLLVGSFLNVVIHRMPKMMLRETANFVAQERGEALPHTGRYDLLLPRSACPHCETPLP
PHHNVPVLSWLLLRGRCGECRAPIGMRYIVVELLTAAVFALAAWKFGAGVTGLAIMACCAVLIALAFIDAETMLLPDDLT
LPLLWLGLLVNTAQRVVPLQDAVLGAAAGYAVLWLIFWIFKLATGKDGLGYGDFKLLAALGAWLGWQALPFVLLSASALG
ALAGIALVVAGRHERGQAIPFGPYLSIAGMLALLYGSDWLSFGIYDY

Nucleotide


Download         Length: 864 bp        

>NTDB_id=210961 BUQ72_RS14095 WP_079217095.1 3177737..3178600(+) (pilD) [Herbaspirillum robiniae strain AA6]
ATGCTCTTCCTCGAGGCTTACGCCGAACCGAACAACGCCTTCTACAGCCTGGCCGCCGCCGTGCTGGGCCTGCTGGTGGG
CAGCTTCCTCAATGTGGTGATCCACCGCATGCCGAAGATGATGCTGCGCGAGACCGCCAACTTCGTCGCCCAGGAGCGCG
GCGAGGCGCTGCCGCACACCGGCCGCTACGACCTGCTGCTGCCGCGCTCGGCCTGCCCGCATTGCGAGACGCCGCTGCCG
CCGCATCACAACGTACCGGTCCTGAGCTGGCTGCTGCTGCGCGGGCGCTGCGGCGAATGCCGCGCGCCCATCGGGATGCG
CTACATCGTCGTCGAACTGCTGACCGCGGCCGTCTTCGCGCTGGCGGCGTGGAAGTTCGGCGCCGGCGTCACCGGGCTGG
CGATCATGGCGTGCTGCGCCGTGCTGATCGCGCTGGCCTTCATCGATGCCGAAACCATGCTGCTGCCCGACGACCTCACG
CTGCCGCTGCTGTGGCTGGGCCTGCTGGTCAACACCGCCCAGCGCGTGGTGCCGCTGCAGGATGCGGTGCTGGGCGCGGC
CGCCGGTTATGCGGTGCTGTGGCTGATCTTCTGGATCTTCAAACTGGCCACCGGCAAGGACGGCCTGGGCTATGGCGACT
TCAAGCTGCTGGCGGCGCTGGGCGCATGGCTGGGCTGGCAGGCGCTGCCCTTCGTGCTGCTGTCGGCCTCGGCGCTGGGC
GCGCTCGCCGGCATCGCGCTGGTGGTGGCCGGCCGCCACGAACGCGGACAGGCGATTCCCTTCGGACCTTATCTGTCGAT
TGCGGGTATGCTGGCGCTGCTTTACGGCAGCGACTGGCTGTCCTTCGGCATCTACGATTACTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(136-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.03

94.425

0.491
  pilD Vibrio campbellii strain DS40M4

47.985

95.122

0.456
  pilD Neisseria gonorrhoeae MS11

49.012

88.153

0.432
  pilD Acinetobacter baumannii D1279779

43.478

96.167

0.418
  pilD Acinetobacter nosocomialis M2

42.909

95.819

0.411

Multiple sequence alignment