Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   TE101_RS14725 Genome accession   NZ_CP018321
Coordinates   3384781..3385212 (+) Length   143 a.a.
NCBI ID   WP_101565570.1    Uniprot ID   -
Organism   Alteromonas macleodii strain Te101     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3379781..3390212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TE101_RS14700 (TE101_14715) pdhR 3380026..3380772 (-) 747 WP_014950397.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  TE101_RS14705 (TE101_14720) ampE 3381138..3382040 (-) 903 WP_101565565.1 beta-lactamase regulator AmpE -
  TE101_RS14710 (TE101_14725) ampD 3382083..3382622 (-) 540 WP_101565567.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  TE101_RS14715 (TE101_14730) - 3382806..3383324 (+) 519 WP_014950400.1 TIGR02281 family clan AA aspartic protease -
  TE101_RS14720 (TE101_14735) nadC 3383357..3384211 (+) 855 WP_101565568.1 carboxylating nicotinate-nucleotide diphosphorylase -
  TE101_RS14725 (TE101_14740) pilA2 3384781..3385212 (+) 432 WP_101565570.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  TE101_RS14730 (TE101_14745) pilC 3385281..3386489 (+) 1209 WP_101565572.1 type II secretion system F family protein Machinery gene
  TE101_RS14735 (TE101_14750) pilD 3386542..3387435 (+) 894 WP_039227988.1 A24 family peptidase Machinery gene
  TE101_RS14740 (TE101_14755) coaE 3387454..3388065 (+) 612 WP_101565574.1 dephospho-CoA kinase -
  TE101_RS14745 (TE101_14760) zapD 3388309..3389061 (+) 753 WP_101565576.1 cell division protein ZapD -
  TE101_RS14750 (TE101_14765) yacG 3389203..3389430 (+) 228 WP_014980027.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14655.77 Da        Isoelectric Point: 4.5635

>NTDB_id=208058 TE101_RS14725 WP_101565570.1 3384781..3385212(+) (pilA2) [Alteromonas macleodii strain Te101]
MMNMNTKNQKGFTLIELMIVVAIIGILAAIALPAYQNYTQKARFTEVTNATAAVKSAVEVCAQTTNDLANCVQNTNGIPA
TVAASAANADPDIVGVTTGANGVITATAHGDMDVPAPGTYVLTPALNGGRVTWTAECTPATLC

Nucleotide


Download         Length: 432 bp        

>NTDB_id=208058 TE101_RS14725 WP_101565570.1 3384781..3385212(+) (pilA2) [Alteromonas macleodii strain Te101]
ATGATGAACATGAATACTAAAAACCAAAAAGGTTTCACCCTAATCGAACTAATGATTGTTGTTGCAATCATCGGTATCCT
TGCAGCAATCGCACTTCCTGCTTACCAAAACTACACGCAAAAAGCGCGTTTTACTGAAGTTACCAATGCAACAGCAGCTG
TAAAATCAGCAGTCGAAGTTTGTGCTCAAACCACTAACGATCTTGCTAACTGTGTACAGAATACGAATGGCATTCCAGCT
ACAGTTGCAGCATCTGCGGCAAATGCTGACCCTGATATTGTAGGCGTTACAACTGGTGCTAACGGCGTAATCACTGCAAC
TGCACATGGCGATATGGATGTACCAGCGCCTGGAACATACGTTTTGACTCCAGCCCTTAACGGTGGTCGAGTAACGTGGA
CAGCAGAGTGCACACCAGCCACTCTTTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

50.36

97.203

0.49
  pilA2 Legionella pneumophila strain ERS1305867

49.64

97.203

0.483
  pilA Haemophilus influenzae 86-028NP

44.755

100

0.448
  pilA Haemophilus influenzae Rd KW20

42.657

100

0.427
  comP Acinetobacter baylyi ADP1

42.254

99.301

0.42
  pilA Vibrio campbellii strain DS40M4

40.411

100

0.413
  pilA Glaesserella parasuis strain SC1401

38.462

100

0.385
  pilA/pilA1 Eikenella corrodens VA1

43.443

85.315

0.371

Multiple sequence alignment