Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BSZ04_RS09730 Genome accession   NZ_CP018312
Coordinates   151106..152839 (+) Length   577 a.a.
NCBI ID   WP_088880305.1    Uniprot ID   -
Organism   Vibrio rotiferianus strain B64D1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 146106..157839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSZ04_RS09710 (BSZ04_09605) pilM 148393..149409 (+) 1017 WP_088880301.1 type IV pilus assembly protein PilM Machinery gene
  BSZ04_RS09715 (BSZ04_09610) pilN 149393..149980 (+) 588 WP_088880302.1 PilN domain-containing protein Machinery gene
  BSZ04_RS09720 (BSZ04_09615) pilO 149973..150566 (+) 594 WP_088880303.1 type 4a pilus biogenesis protein PilO Machinery gene
  BSZ04_RS09725 (BSZ04_09620) pilP 150556..151068 (+) 513 WP_088880304.1 pilus assembly protein PilP Machinery gene
  BSZ04_RS09730 (BSZ04_09625) pilQ 151106..152839 (+) 1734 WP_088880305.1 type IV pilus secretin PilQ Machinery gene
  BSZ04_RS09735 (BSZ04_09630) aroK 153028..153546 (+) 519 WP_010443904.1 shikimate kinase AroK -
  BSZ04_RS09740 (BSZ04_09635) aroB 153578..154678 (+) 1101 WP_010443901.1 3-dehydroquinate synthase -
  BSZ04_RS09745 (BSZ04_09640) - 154730..156250 (+) 1521 WP_088880306.1 SPOR domain-containing protein -
  BSZ04_RS09750 (BSZ04_09645) - 156320..157159 (+) 840 WP_010443897.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63417.38 Da        Isoelectric Point: 4.4923

>NTDB_id=207980 BSZ04_RS09730 WP_088880305.1 151106..152839(+) (pilQ) [Vibrio rotiferianus strain B64D1]
MKQGNTLRYRKLLELLWLCGLLLFPPAVWAEESQSNALRSIDFRSNKNKEAVIVVELASPAAIVDVKRVQEGLSIDLLNT
SVKDDQLYLLDVKDFATAVDSVEVFRDAATTRLLAHIEGDYTHDYRLTGRYLEVKVARLKPNEKVPDKSILEKEGKLISI
NFQDIPVRNVLQLIADYNDFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KQKLEEEIGELASEIIKVNFAKASDIAEMINGEGNISMLSNRGSMTIDERTNSLLIRELPENIEVIRDIVESLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSINGNNMVGGSIENNLAAIGLYDEGGDNEDGNTVGIDDFLNVNLAATSPSATSIAF
QVAKLGSDTLLDLELSALQQESKAEIISSPRLVTTNKKPAYIEQGTEIPYLEASSSGATSITFKKAVLSLKVTPQITPDN
RLVLDLSVTQDRLGKVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHNVTNTVDKVPLLGDLPLLGALFRRSYEN
VGKSELLIFVTPKVVVQ

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=207980 BSZ04_RS09730 WP_088880305.1 151106..152839(+) (pilQ) [Vibrio rotiferianus strain B64D1]
ATGAAACAAGGAAATACATTGAGATACAGGAAGTTATTAGAACTCTTATGGTTGTGTGGACTACTATTGTTTCCCCCAGC
TGTTTGGGCTGAGGAGTCTCAGTCTAACGCCCTGAGAAGTATTGATTTCAGAAGTAACAAAAATAAAGAGGCGGTCATTG
TTGTTGAGCTCGCATCGCCTGCCGCTATCGTTGATGTAAAACGAGTTCAAGAAGGGTTGAGTATCGACCTTCTTAATACG
TCTGTGAAAGATGATCAACTTTACCTTCTCGATGTCAAAGACTTTGCAACTGCCGTCGATAGTGTTGAAGTCTTCCGTGA
TGCTGCAACAACTCGGTTATTGGCGCACATTGAAGGGGACTATACACATGATTACAGGCTTACTGGGCGATATCTCGAAG
TCAAAGTTGCACGCTTAAAACCGAATGAAAAGGTGCCAGATAAAAGTATCCTTGAGAAGGAAGGCAAGCTCATCTCTATT
AACTTTCAAGATATACCGGTAAGAAATGTCTTGCAGTTGATCGCTGACTACAACGATTTTAATTTGGTGGTCTCGGATTC
TGTTGAGGGTAACTTAACGTTACGCCTGGATGGTGTGCCATGGCAGCAGGTACTAGACATTATTCTACAGGTTAAGGGAC
TTGATAAACGCGTTGATGGCAACGTTATTTTAGTCGCACCAAAAGAAGAACTGGACTTACGTGAAAAGCAGCAATTGGAG
AAGCAAAAATTAGAAGAGGAAATTGGAGAGCTAGCATCAGAAATCATTAAAGTAAATTTTGCAAAAGCTTCGGATATTGC
GGAGATGATTAATGGTGAAGGGAATATTAGTATGCTTTCCAATCGAGGTAGTATGACAATCGATGAGCGCACTAACTCTC
TGCTTATTCGTGAGCTGCCAGAGAATATCGAAGTAATACGTGACATCGTAGAGTCACTAGATATACCCGTCAAGCAGGTT
CAAATAGAGGCCAGAATTGTAACCGTGAATGAAGGTAACCTTGATGAACTTGGTGTTCGCTGGGGCTTTAGTTCCATAAA
TGGTAACAACATGGTAGGTGGTTCGATTGAGAACAATCTTGCTGCTATTGGCCTTTATGACGAAGGTGGTGATAATGAAG
ATGGTAATACCGTTGGTATTGATGATTTCTTAAATGTAAACCTAGCGGCGACTTCGCCTAGCGCAACGAGTATTGCCTTT
CAAGTTGCTAAGTTGGGCTCTGATACGCTGTTAGATCTGGAGCTTTCCGCTCTACAACAAGAATCTAAGGCTGAAATCAT
CTCAAGTCCGCGTTTGGTAACGACAAACAAAAAGCCTGCCTATATTGAGCAAGGTACAGAAATTCCTTATCTCGAAGCAT
CGTCCAGCGGGGCGACTTCCATCACTTTCAAAAAAGCAGTATTAAGTCTAAAAGTCACGCCACAAATAACCCCAGACAAT
CGTTTAGTTCTCGATCTGAGTGTAACCCAAGATCGTTTAGGTAAGGTTGTCAAAACAGGTACTGGGGAAGCCGTTGCAAT
TGATACGCAGCGCATAGGAACTCAAGTATTAGTCAATAATGGTGAAACCGTCGTTTTAGGTGGAATATTTCAGCACAATG
TGACTAATACTGTGGACAAGGTTCCTCTGCTTGGAGATCTCCCATTGCTAGGGGCGTTATTTCGCAGAAGTTATGAAAAT
GTGGGTAAGAGTGAGCTACTGATCTTTGTTACACCTAAGGTAGTAGTTCAGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(254-319)

Bacterial type II and III secretion system protein (Secretin)

(417-575)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

87.37

100

0.875
  pilQ Vibrio cholerae strain A1552

74.567

100

0.747
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.567

100

0.747
  pilQ Pseudomonas aeruginosa PAK

41.953

95.841

0.402
  pilQ Legionella pneumophila strain Lp02

40.506

95.841

0.388
  pilQ Legionella pneumophila strain ERS1305867

40.506

95.841

0.388
  comQ Acinetobacter baylyi ADP1

38.062

100

0.381
  pilQ Acinetobacter baumannii strain A118

36.905

100

0.376
  pilQ Acinetobacter baumannii D1279779

36.905

100

0.376

Multiple sequence alignment