Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BSZ04_RS09710 Genome accession   NZ_CP018312
Coordinates   148393..149409 (+) Length   338 a.a.
NCBI ID   WP_088880301.1    Uniprot ID   -
Organism   Vibrio rotiferianus strain B64D1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 143393..154409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSZ04_RS09695 (BSZ04_09590) - 143795..144523 (-) 729 WP_010443933.1 redoxin family protein -
  BSZ04_RS09700 (BSZ04_09595) oxyR 144698..145606 (+) 909 WP_010443932.1 DNA-binding transcriptional regulator OxyR -
  BSZ04_RS09705 (BSZ04_09600) - 145685..148252 (-) 2568 WP_088880300.1 penicillin-binding protein 1A -
  BSZ04_RS09710 (BSZ04_09605) pilM 148393..149409 (+) 1017 WP_088880301.1 type IV pilus assembly protein PilM Machinery gene
  BSZ04_RS09715 (BSZ04_09610) pilN 149393..149980 (+) 588 WP_088880302.1 PilN domain-containing protein Machinery gene
  BSZ04_RS09720 (BSZ04_09615) pilO 149973..150566 (+) 594 WP_088880303.1 type 4a pilus biogenesis protein PilO Machinery gene
  BSZ04_RS09725 (BSZ04_09620) pilP 150556..151068 (+) 513 WP_088880304.1 pilus assembly protein PilP Machinery gene
  BSZ04_RS09730 (BSZ04_09625) pilQ 151106..152839 (+) 1734 WP_088880305.1 type IV pilus secretin PilQ Machinery gene
  BSZ04_RS09735 (BSZ04_09630) aroK 153028..153546 (+) 519 WP_010443904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37729.10 Da        Isoelectric Point: 5.8905

>NTDB_id=207976 BSZ04_RS09710 WP_088880301.1 148393..149409(+) (pilM) [Vibrio rotiferianus strain B64D1]
MDKLIVTGIDITHRSLKAVILKPTGKSFSLLGYKEIVLNGDIVAENQLINHQEIVNTLKEIKKSLPLFHRKVAIAVPDSA
VISKVLPLDCDLEESEIEFAVAQAFSLQSPIPIDELSLDYVPCDGGTMTKNRQYQVFATRKELVDSRVKLLEKVGLKPAV
VDVHAQVAGEVWQLAAEANPENNSFCLVEVRERDGSITMFNTEGELFHKEFTWSPSEPLVQDSNFSEGGEAEIKATDHFN
QDVAKNIQRQLQLYSSVSGSNDVQGLWLSGSRASTPMLLETLSTCLGIRCEILNIFEHFEIQLSPRRRRDLDWHRFTTAA
GIAVRAIKCLRRGNASTR

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=207976 BSZ04_RS09710 WP_088880301.1 148393..149409(+) (pilM) [Vibrio rotiferianus strain B64D1]
ATGGATAAGTTAATAGTTACTGGCATTGATATTACTCATCGCAGCCTCAAAGCCGTAATACTAAAACCAACAGGGAAGAG
CTTTTCCCTGCTTGGCTATAAAGAAATTGTACTAAACGGCGACATTGTCGCTGAAAACCAACTCATAAATCATCAAGAAA
TTGTAAATACCCTAAAAGAGATAAAAAAGTCGCTACCACTATTTCATCGAAAAGTAGCGATAGCTGTACCGGACAGTGCT
GTTATCTCAAAGGTTTTGCCGTTGGACTGTGACTTAGAAGAGAGCGAAATTGAGTTTGCAGTGGCACAAGCCTTTTCTCT
TCAATCTCCCATTCCTATAGATGAGCTGAGTTTGGATTATGTCCCTTGTGATGGGGGCACTATGACTAAGAATAGGCAAT
ATCAGGTCTTTGCGACGAGAAAGGAATTAGTAGATAGCCGAGTTAAGCTGTTGGAAAAAGTAGGATTAAAACCTGCAGTA
GTTGATGTGCATGCTCAGGTCGCGGGAGAAGTATGGCAATTAGCTGCTGAGGCCAACCCTGAAAACAACAGTTTTTGCCT
TGTAGAAGTAAGAGAGCGTGATGGTTCGATAACGATGTTTAATACAGAAGGAGAGTTGTTTCATAAAGAGTTTACGTGGA
GCCCTTCTGAGCCATTAGTTCAAGATAGCAATTTCTCTGAAGGAGGAGAGGCTGAAATAAAAGCTACCGATCACTTTAAT
CAAGACGTTGCCAAAAACATTCAAAGGCAGCTTCAGCTTTACTCTTCTGTATCGGGCTCAAATGACGTACAAGGCCTTTG
GTTATCAGGCTCAAGGGCATCAACACCAATGCTATTAGAAACGCTCTCCACTTGCTTAGGTATTCGTTGTGAGATTCTTA
ATATATTCGAACATTTCGAAATTCAGCTCTCACCAAGGCGCCGTAGGGATTTAGATTGGCACCGGTTCACTACGGCGGCA
GGTATTGCTGTCAGAGCAATAAAGTGCTTGAGGAGGGGGAATGCTTCAACCCGTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-210)

Type IV pilus assembly protein PilM; (PilM_2)

(238-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

54.36

100

0.553
  pilM Vibrio cholerae strain A1552

40.87

100

0.417

Multiple sequence alignment