Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BSR56_RS04790 Genome accession   NZ_CP018260
Coordinates   964159..965277 (-) Length   372 a.a.
NCBI ID   WP_100833382.1    Uniprot ID   -
Organism   Acinetobacter haemolyticus strain XH900     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 959159..970277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSR56_RS04760 (BSR56_04785) - 959837..960685 (+) 849 WP_004638208.1 class II glutamine amidotransferase -
  BSR56_RS04765 (BSR56_04790) - 960960..961415 (+) 456 WP_008940857.1 bacteriohemerythrin -
  BSR56_RS04770 (BSR56_04795) - 961665..962747 (+) 1083 WP_075316017.1 hypothetical protein -
  BSR56_RS04775 (BSR56_04800) - 962747..963067 (+) 321 WP_005091348.1 RnfH family protein -
  BSR56_RS04780 (BSR56_04805) - 963124..963522 (-) 399 WP_004638212.1 outer membrane protein assembly factor BamE -
  BSR56_RS04785 (BSR56_04810) fur 963634..964071 (+) 438 WP_004638213.1 ferric iron uptake transcriptional regulator -
  BSR56_RS04790 (BSR56_04815) pilU 964159..965277 (-) 1119 WP_100833382.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BSR56_RS04795 (BSR56_04820) pilT 965306..966343 (-) 1038 WP_004638216.1 type IV pilus twitching motility protein PilT Machinery gene
  BSR56_RS04800 (BSR56_04825) - 966470..967156 (+) 687 WP_100833383.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41744.82 Da        Isoelectric Point: 6.2881

>NTDB_id=207821 BSR56_RS04790 WP_100833382.1 964159..965277(-) (pilU) [Acinetobacter haemolyticus strain XH900]
MDFNDLLNLMIQQNASDLFITADVEPSMKINGQIVPVSKTKLSAEIVGQLVNSIMSDKQRKEFADTRECNFAITNREKTA
RFRVSAFQQRDMPGMILRKIETHIPSMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNHNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLKALVAQQLIPTPDGNSRRAAIEILINTPLLADYIRNGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLSEEGADQLLNASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=207821 BSR56_RS04790 WP_100833382.1 964159..965277(-) (pilU) [Acinetobacter haemolyticus strain XH900]
ATGGATTTTAATGATTTACTCAATTTGATGATTCAACAAAACGCATCTGATTTATTTATCACTGCGGATGTTGAACCATC
TATGAAAATTAACGGGCAAATTGTGCCTGTTTCCAAAACCAAATTATCTGCTGAAATTGTGGGGCAACTTGTCAATTCAA
TTATGAGTGACAAGCAACGTAAAGAATTTGCAGATACACGTGAGTGTAACTTCGCAATTACTAATCGTGAAAAAACAGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGCGATATGCCTGGGATGATTTTACGTAAAATCGAAACCCATATTCCGAGTAT
GGACGATTTAAAACTGCCTGAAGTACTCAAAGAACTTGCTATGACCAAACGTGGCATTATTATCTTTGTTGGCGCAACAG
GTACAGGTAAATCTACTTCCCTCGCTTCAATGATTGGTTATCGTAATCACAATTCCAAAGGACACATTATCACCATCGAA
GATCCGATTGAATTTGTGCATCAACATGCGGGGTGTATCGTCACGCAACGTGAAGTCGGGATTGATACTGAATCTTTTGA
AGTTGCATTAAAAAATACACTTCGTCAGGCGCCAGATGTCATTTTGATTGGTGAGATTCGTTCACGTGAAACCATGGACT
ATGCGATCGCCTTTGCTGAAACAGGTCACTTGGTCTTGGCAACGCTTCACGCCAACAATGCCAACCAAGCCCTAGATCGT
ATTATTCACTTCTTCGAAGCCGATCGTCATAGTCAGTTGTATATGGATCTGTCACTTAACTTAAAGGCGTTGGTCGCGCA
ACAATTGATCCCAACACCTGATGGAAATTCACGTCGTGCTGCGATTGAAATTTTGATCAATACTCCTTTATTGGCTGACT
ATATTCGTAACGGTGAAATCCATGAGATCAAAGATCTGATGAAACGCTCTCGTGAATTGGGTATGCAAACCTTTGACCAA
GCCCTATTTGACCTCTATAAAGCGGGACAAATCACTTACAAAGATGCATTAAAGCATGCAGACTCACCAAACGACTTGCG
TCTCACCATTAAGCTTTCTGAAGAAGGCGCAGATCAACTACTGAATGCGAGCCGTAACATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.811

99.731

0.696
  pilU Vibrio cholerae strain A1552

54.324

99.462

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.385

97.849

0.395
  pilT Legionella pneumophila strain Lp02

41.42

90.86

0.376
  pilT Legionella pneumophila strain ERS1305867

41.42

90.86

0.376
  pilT Neisseria meningitidis 8013

40.294

91.398

0.368
  pilT Neisseria gonorrhoeae MS11

40.294

91.398

0.368
  pilT Pseudomonas aeruginosa PAK

39.017

93.011

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36

Multiple sequence alignment