Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BO996_RS25960 Genome accession   NZ_CP018101
Coordinates   5805108..5806325 (+) Length   405 a.a.
NCBI ID   WP_071956730.1    Uniprot ID   -
Organism   Delftia sp. HK171     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5800108..5811325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BO996_RS25935 (BO996_25540) rpmA 5801342..5801599 (-) 258 WP_012207283.1 50S ribosomal protein L27 -
  BO996_RS25940 (BO996_25545) rplU 5801615..5801926 (-) 312 WP_012207284.1 50S ribosomal protein L21 -
  BO996_RS25945 (BO996_25550) - 5802157..5803086 (+) 930 WP_034396917.1 polyprenyl synthetase family protein -
  BO996_RS25955 (BO996_25560) pilB 5803327..5805069 (+) 1743 WP_071956729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BO996_RS25960 (BO996_25565) pilC 5805108..5806325 (+) 1218 WP_071956730.1 type II secretion system F family protein Machinery gene
  BO996_RS25965 (BO996_25570) pilD 5806325..5807212 (+) 888 WP_071956731.1 A24 family peptidase Machinery gene
  BO996_RS25970 (BO996_25575) coaE 5807227..5807871 (+) 645 WP_071956732.1 dephospho-CoA kinase -
  BO996_RS25975 (BO996_25580) zapD 5807961..5808716 (+) 756 WP_012207290.1 cell division protein ZapD -
  BO996_RS25980 (BO996_25585) - 5808734..5808970 (+) 237 WP_156898007.1 DNA gyrase inhibitor YacG -
  BO996_RS25985 (BO996_25590) - 5808981..5809499 (-) 519 WP_065345741.1 NUDIX domain-containing protein -
  BO996_RS25990 (BO996_25595) - 5809550..5810506 (-) 957 WP_071956733.1 ATP-binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44488.33 Da        Isoelectric Point: 9.3922

>NTDB_id=205814 BO996_RS25960 WP_071956730.1 5805108..5806325(+) (pilC) [Delftia sp. HK171]
MATAASKGIKEFVFEWEGKDRNGKIIRGETRAGGINQVQAMLRRQGVLPSKIKKRRTRGGKRIKPKDIALFTRQMATMMK
AGVPLLQAFDIVGRGNTNPSVTRLLADIRSDVETGTSLNAAYRKHPMYFDSLYCNLVEAGEAAGILEALLDRLATYMEKT
EAIKSKIKSALMYPISVVVVAFVVVTIIMIFVIPAFKEVFTSFGADLPAPTLFVMGISDIFVQWWWVIFGVIGGGFYFFM
QAWKRNERMQQFMDRAMLKMPVFGVLIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNAVYANATEKIQQEVSTGT
SLTVAMTNANVFPSMVLQMCAIGEESGSIDHMLGKAADFYEEEVDDMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=205814 BO996_RS25960 WP_071956730.1 5805108..5806325(+) (pilC) [Delftia sp. HK171]
ATGGCCACAGCCGCGTCCAAGGGAATCAAGGAATTTGTCTTCGAGTGGGAAGGCAAGGATCGCAACGGCAAGATCATTCG
AGGCGAAACCCGGGCCGGAGGCATCAACCAGGTCCAGGCAATGCTGCGCCGCCAGGGCGTGCTCCCCAGCAAGATCAAGA
AGCGCCGTACGCGCGGTGGCAAAAGAATCAAGCCCAAGGACATCGCGCTGTTCACGCGCCAGATGGCCACGATGATGAAG
GCTGGTGTCCCGCTGCTGCAGGCCTTCGACATCGTCGGGCGCGGCAACACCAACCCCAGTGTGACGCGCCTGCTGGCCGA
TATCCGCTCCGACGTGGAGACAGGCACCTCGCTGAATGCGGCCTATCGCAAGCACCCCATGTACTTCGATAGCCTCTACT
GCAATCTGGTGGAGGCCGGGGAAGCGGCCGGTATCCTGGAGGCCCTGCTCGATCGGCTGGCCACCTATATGGAAAAAACC
GAGGCCATCAAGTCCAAGATCAAGTCCGCCTTGATGTATCCGATTTCGGTGGTCGTCGTGGCTTTCGTCGTCGTGACCAT
CATCATGATCTTCGTGATTCCCGCCTTCAAGGAAGTGTTCACCTCCTTCGGCGCGGATCTGCCGGCACCGACGCTGTTCG
TCATGGGCATCAGCGACATCTTTGTTCAATGGTGGTGGGTGATCTTCGGCGTGATCGGAGGCGGCTTCTACTTCTTCATG
CAGGCCTGGAAGCGCAATGAGCGCATGCAGCAGTTCATGGATCGTGCAATGTTGAAGATGCCGGTCTTTGGCGTGCTGAT
CGAAAAGTCCTGCGTTGCGCGCTGGACGCGCACGCTGTCGACCATGTTTGCGGCAGGCGTTCCGCTGGTCGAGGCACTGG
ATTCCGTCGGCGGCGCCTCCGGGAATGCCGTCTACGCCAATGCCACCGAGAAGATCCAGCAGGAAGTCTCCACCGGCACC
AGCCTCACGGTAGCCATGACCAATGCCAACGTCTTTCCCTCCATGGTGCTGCAGATGTGCGCCATCGGCGAGGAATCCGG
CTCCATCGACCACATGCTTGGCAAGGCGGCCGATTTCTACGAGGAGGAGGTCGATGACATGGTGGCTGGCCTGTCCAGCC
TGATGGAGCCCATCATCATTGTTTTCCTGGGCACCCTCATCGGCGGCATCGTGGTTTCGATGTATCTGCCCATCTTCAAG
CTGGGTCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.446

99.753

0.553
  pilG Neisseria gonorrhoeae MS11

50

98.765

0.494
  pilC Legionella pneumophila strain ERS1305867

50.251

98.272

0.494
  pilG Neisseria meningitidis 44/76-A

49.75

98.765

0.491
  pilC Acinetobacter baylyi ADP1

49.246

98.272

0.484
  pilC Acinetobacter baumannii D1279779

47.859

98.025

0.469
  pilC Vibrio cholerae strain A1552

40.302

98.025

0.395
  pilC Vibrio campbellii strain DS40M4

39.5

98.765

0.39
  pilC Thermus thermophilus HB27

37.093

98.519

0.365

Multiple sequence alignment