Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BO996_RS25955 Genome accession   NZ_CP018101
Coordinates   5803327..5805069 (+) Length   580 a.a.
NCBI ID   WP_071956729.1    Uniprot ID   -
Organism   Delftia sp. HK171     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5798327..5810069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BO996_RS25925 (BO996_25530) proB 5798845..5799993 (-) 1149 WP_012207281.1 glutamate 5-kinase -
  BO996_RS25930 (BO996_25535) obgE 5800077..5801192 (-) 1116 WP_071956728.1 GTPase ObgE -
  BO996_RS25935 (BO996_25540) rpmA 5801342..5801599 (-) 258 WP_012207283.1 50S ribosomal protein L27 -
  BO996_RS25940 (BO996_25545) rplU 5801615..5801926 (-) 312 WP_012207284.1 50S ribosomal protein L21 -
  BO996_RS25945 (BO996_25550) - 5802157..5803086 (+) 930 WP_034396917.1 polyprenyl synthetase family protein -
  BO996_RS25955 (BO996_25560) pilB 5803327..5805069 (+) 1743 WP_071956729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BO996_RS25960 (BO996_25565) pilC 5805108..5806325 (+) 1218 WP_071956730.1 type II secretion system F family protein Machinery gene
  BO996_RS25965 (BO996_25570) pilD 5806325..5807212 (+) 888 WP_071956731.1 A24 family peptidase Machinery gene
  BO996_RS25970 (BO996_25575) coaE 5807227..5807871 (+) 645 WP_071956732.1 dephospho-CoA kinase -
  BO996_RS25975 (BO996_25580) zapD 5807961..5808716 (+) 756 WP_012207290.1 cell division protein ZapD -
  BO996_RS25980 (BO996_25585) - 5808734..5808970 (+) 237 WP_156898007.1 DNA gyrase inhibitor YacG -
  BO996_RS25985 (BO996_25590) - 5808981..5809499 (-) 519 WP_065345741.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62722.81 Da        Isoelectric Point: 5.2400

>NTDB_id=205813 BO996_RS25955 WP_071956729.1 5803327..5805069(+) (pilB) [Delftia sp. HK171]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAASKAGANRTSFIAELSNAGILSALEVAETVSTMFSAPLLDLD
AINAARLPNDLLDAKIISSYRVLPLSKRGNRITVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQSKSASET
MESYSGGGDFEFGDLKPDEVAEEKDNTAATDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLIEAIGRPYGMILVTGPTGSGKTVSLYTCLNMLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALLDAGYDEADIDGSWVTYKPVGCQACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=205813 BO996_RS25955 WP_071956729.1 5803327..5805069(+) (pilB) [Delftia sp. HK171]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCTTCCGCTGTTGCATCGCTTCCCGGCATCGGGCGAGCCCTGATATCAGC
AGGCAAGGTATCGCAGCAGGCAGCGGAAGCCGCCGCCAGCAAGGCCGGCGCCAACCGCACCTCGTTCATTGCCGAGTTGA
GCAACGCGGGCATCCTCTCGGCCCTGGAAGTGGCCGAGACGGTCTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCCATCAACGCCGCGCGCCTGCCGAATGACTTGCTGGACGCCAAGATCATCAGCTCCTATCGCGTCCTCCCCTTGAGCAA
GCGCGGCAACCGCATCACGGTGGCCACGGCCGACCCGACACAGCAGGAAGCTGCAGAGCAGATCAAGTTCACCACCCAGC
TAGGCGTGGATTGGATCATCGTCGAGGCCGACAAGCTGGAACGTCTGATAGCTCAGCAGAGCAAAAGCGCCAGCGAAACC
ATGGAGAGCTACAGCGGGGGCGGCGATTTCGAGTTCGGCGACCTCAAGCCCGACGAGGTTGCCGAGGAGAAGGACAACAC
GGCGGCAACGGACGTCGAGGACGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACCTGCATTTCGAGCCCTACGAACATCAGTACCGCGTACGCTTCCGGATTGACGGCGAGTTGCGTGAGATTGCATCG
CCCCCCATTGCGATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGGGTTCC
GCAAGACGGGCGCATGAAGCTCAAGGTGGGTCCGGACCGCGTCATCGACTTTCGCGTGAGCACCCTGCCCACGCTCTTCG
GCGAGAAGATCGTGATCCGTATCCTTGACCCGAGCTCGGCCAAGATGGGCATTGATGCCCTGGGCTACGAACCAGAGGAA
AAGGCTCGCCTGATCGAAGCCATCGGGCGCCCCTACGGAATGATTCTGGTGACAGGCCCCACCGGCTCGGGCAAGACGGT
GTCGCTGTACACCTGCCTGAACATGCTGAACAAGGCCGGGGTGAACATCGCGACGGCAGAAGACCCCTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAACGTGAACGAAAAGGCAGGCCTCACCTTTGCGACGGCGCTGAAGGCCTTCCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGTCA
CCTGGTGCTGTCCACGCTGCACACCAACGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCCCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGGCTGGCCAGGCGCCTGTGCCCCCAGTGCAAGGAGCCTGCGGAT
ATCCCGCGGGAGGCGCTGCTGGATGCGGGCTATGACGAGGCGGACATTGATGGCAGCTGGGTCACCTACAAGCCCGTGGG
CTGCCAGGCATGCAACAACGGCTACAAGGGACGCGTCGGCATCTACCAGGTGATGCCGATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGCGCGCTGGAGATCGCGGCACAGGCCAAGCGTGAAGGCGTGCGGTCTCTGCGCGGATCCGGA
CTGCACAAGGCCAGACTAGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilB Acinetobacter baumannii D1279779

56.028

97.241

0.545
  pilF Neisseria gonorrhoeae MS11

51.943

97.586

0.507
  pilB Legionella pneumophila strain ERS1305867

50.264

98.103

0.493
  pilB Vibrio cholerae strain A1552

46.279

95

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

45.372

95

0.431
  pilB Vibrio campbellii strain DS40M4

43.54

97.414

0.424
  pilF Thermus thermophilus HB27

38.22

98.793

0.378

Multiple sequence alignment