Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BKK80_RS18500 Genome accession   NZ_CP017754
Coordinates   4083381..4084646 (+) Length   421 a.a.
NCBI ID   WP_071015565.1    Uniprot ID   -
Organism   Cupriavidus malaysiensis strain USMAA1020 isolate pure     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4078381..4089646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKK80_RS18475 (BKK80_18475) rpmA 4079036..4079296 (-) 261 WP_071015560.1 50S ribosomal protein L27 -
  BKK80_RS18480 (BKK80_18480) rplU 4079357..4079668 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  BKK80_RS18485 (BKK80_18485) ispB 4080313..4081242 (+) 930 WP_071015561.1 octaprenyl diphosphate synthase -
  BKK80_RS18495 (BKK80_18495) pilF 4081616..4083352 (+) 1737 WP_071070424.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKK80_RS18500 (BKK80_18500) pilC 4083381..4084646 (+) 1266 WP_071015565.1 type II secretion system F family protein Machinery gene
  BKK80_RS18505 (BKK80_18505) pilD 4084650..4085543 (+) 894 WP_071015568.1 prepilin peptidase Machinery gene
  BKK80_RS18510 (BKK80_18510) coaE 4085598..4086230 (+) 633 WP_071015570.1 dephospho-CoA kinase -
  BKK80_RS18515 (BKK80_18515) zapD 4086438..4087196 (+) 759 WP_071016592.1 cell division protein ZapD -
  BKK80_RS18520 (BKK80_18520) - 4087281..4087472 (+) 192 WP_071015571.1 DNA gyrase inhibitor YacG -
  BKK80_RS18525 (BKK80_18525) - 4087513..4087941 (-) 429 WP_071015573.1 NUDIX domain-containing protein -
  BKK80_RS18530 (BKK80_18530) - 4087941..4088831 (-) 891 WP_071015574.1 ATP-binding protein -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46395.52 Da        Isoelectric Point: 10.2638

>NTDB_id=202547 BKK80_RS18500 WP_071015565.1 4083381..4084646(+) (pilC) [Cupriavidus malaysiensis strain USMAA1020 isolate pure]
MATRAPAAAARTAAPSRSKPGRKAATQYIFEWEGKDRKGKTFTGEQRAEDQAEVNAMLRKQGLTVVKMKRRRAARGRKIT
EKDIAYFTRQLSTMLKAGVPLLQSIDIIARGHANPNFTQLLAQIRFDIEAGSSMAQAFRRHPRYFDTLYCNLIDAGEQGG
ILDALLERLSMYMEKTIALKSQIKSAMIYPIAVLTVAFAVTVILMLFVIPAFKGVFASFGANLPAPTLFVIAISDFFVAY
WYIVIGVPVAAIALYLRALRKSEKVQRATDRALLKMPIFGSLFRKAVIARWTRTLATMFAAGTPLVESMESVAGAAGNWV
YHDATREVEQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVAAISSLIEPLIIVILG
VLIGGMVIAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=202547 BKK80_RS18500 WP_071015565.1 4083381..4084646(+) (pilC) [Cupriavidus malaysiensis strain USMAA1020 isolate pure]
ATGGCAACGCGTGCACCAGCCGCGGCGGCCCGCACGGCAGCGCCGTCGCGGAGCAAGCCGGGGCGCAAGGCGGCGACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAGGGCAAGACCTTCACCGGGGAACAGCGCGCGGAGGACCAGGCCGAAG
TCAATGCCATGCTGCGCAAGCAGGGCCTGACGGTGGTCAAGATGAAGCGCCGCCGCGCGGCGCGCGGTCGCAAGATCACC
GAGAAGGACATCGCCTACTTCACGCGCCAGTTGTCCACCATGCTCAAGGCAGGCGTGCCGCTGCTGCAGTCGATCGACAT
CATCGCGCGCGGCCATGCCAACCCGAACTTCACCCAGCTGCTGGCGCAGATCCGCTTCGACATCGAGGCCGGCAGCAGCA
TGGCGCAGGCCTTCCGGCGCCATCCGCGCTACTTCGACACGCTCTACTGCAACCTGATCGACGCCGGCGAACAGGGCGGC
ATCCTGGATGCGCTGCTCGAACGACTGTCGATGTACATGGAGAAGACGATCGCGCTGAAAAGCCAGATCAAGTCGGCCAT
GATCTACCCGATCGCGGTGCTGACCGTGGCCTTCGCCGTCACCGTGATCCTGATGCTGTTCGTGATCCCTGCCTTCAAGG
GCGTGTTCGCCAGCTTCGGCGCCAACCTGCCGGCGCCGACGCTGTTCGTGATCGCCATCTCCGACTTCTTCGTGGCCTAC
TGGTACATCGTCATCGGCGTGCCGGTGGCGGCCATCGCACTGTACCTGCGCGCCTTGCGCAAGTCGGAGAAGGTGCAGCG
CGCCACCGACCGCGCGCTGCTGAAGATGCCCATCTTCGGCAGCCTGTTCCGCAAGGCGGTGATCGCGCGCTGGACGCGCA
CCCTGGCCACCATGTTCGCCGCGGGCACGCCGCTGGTGGAGTCGATGGAGTCGGTGGCGGGCGCGGCCGGCAACTGGGTC
TACCACGACGCCACGCGCGAGGTCGAACAGGCGGTGCGCATCGGCACCAGCCTGACCAACGCCATGCAGGCCACCCACGT
GTTCGACAATATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCGGGCGCGCTGGACAATATGCTGCTGAAGGTGGCCG
AATTCTACGAGCGCGAGGTCGACGACGCGGTCGCGGCCATCTCCAGCCTGATCGAGCCGCTGATCATCGTGATCCTCGGG
GTACTGATCGGCGGCATGGTGATTGCCATGTACCTGCCGATCTTCAAGCTGGGACAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

50.372

95.724

0.482
  pilG Neisseria gonorrhoeae MS11

50.376

94.774

0.477
  pilG Neisseria meningitidis 44/76-A

50.125

94.774

0.475
  pilC Acinetobacter baumannii D1279779

48.642

96.2

0.468
  pilC Acinetobacter baylyi ADP1

47.901

96.2

0.461
  pilC Legionella pneumophila strain ERS1305867

46.97

94.062

0.442
  pilC Vibrio cholerae strain A1552

40.602

94.774

0.385
  pilC Thermus thermophilus HB27

37.59

98.575

0.371
  pilC Vibrio campbellii strain DS40M4

38.791

94.299

0.366

Multiple sequence alignment