Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BKK80_RS18495 Genome accession   NZ_CP017754
Coordinates   4081616..4083352 (+) Length   578 a.a.
NCBI ID   WP_071070424.1    Uniprot ID   -
Organism   Cupriavidus malaysiensis strain USMAA1020 isolate pure     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4076616..4088352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKK80_RS18470 (BKK80_18470) obgE 4077793..4078893 (-) 1101 WP_071015558.1 GTPase ObgE -
  BKK80_RS18475 (BKK80_18475) rpmA 4079036..4079296 (-) 261 WP_071015560.1 50S ribosomal protein L27 -
  BKK80_RS18480 (BKK80_18480) rplU 4079357..4079668 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  BKK80_RS18485 (BKK80_18485) ispB 4080313..4081242 (+) 930 WP_071015561.1 octaprenyl diphosphate synthase -
  BKK80_RS18495 (BKK80_18495) pilF 4081616..4083352 (+) 1737 WP_071070424.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKK80_RS18500 (BKK80_18500) pilC 4083381..4084646 (+) 1266 WP_071015565.1 type II secretion system F family protein Machinery gene
  BKK80_RS18505 (BKK80_18505) pilD 4084650..4085543 (+) 894 WP_071015568.1 prepilin peptidase Machinery gene
  BKK80_RS18510 (BKK80_18510) coaE 4085598..4086230 (+) 633 WP_071015570.1 dephospho-CoA kinase -
  BKK80_RS18515 (BKK80_18515) zapD 4086438..4087196 (+) 759 WP_071016592.1 cell division protein ZapD -
  BKK80_RS18520 (BKK80_18520) - 4087281..4087472 (+) 192 WP_071015571.1 DNA gyrase inhibitor YacG -
  BKK80_RS18525 (BKK80_18525) - 4087513..4087941 (-) 429 WP_071015573.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63331.89 Da        Isoelectric Point: 7.8159

>NTDB_id=202546 BKK80_RS18495 WP_071070424.1 4081616..4083352(+) (pilF) [Cupriavidus malaysiensis strain USMAA1020 isolate pure]
MTLGLALAQSRRIAPALLAQLEQAAREKKSQLIDEIVGSGTMSARDLATFAADKYQLPLLDLSQFNFATVPPALVGNREF
HAHRLLPLGRRDNRLVVATSDPSNQAGLDAIRQKLNLPVDAVVVEHDKLLKHVRSAGEESSTLRAAGPVAAEHKLIEYDP
VASAGAQRNKAPNDIDDAPVVRFLHKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLLEVARPPLDIRDKIATRIKVLS
RLDISEKRVPQDGRMKLLIALPKEKDKDGKDSKETVEKAIDFRVSTLPTLFGEKIVMRILDSSTDKLDIDRLGYEPAQKA
LLLETIKRPYGMILVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNEKAGLTFAAALRSFLRQDP
DVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCNCKRPGALAR
DVLLDAGFHESELDGSWQPYHPAGCERCSGSGYKGRCGIYQVMPISEAMQQIILSHGTALQIAEQARRDGVLSLREAGLL
KVKQGVTSLEEVLATTNM

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=202546 BKK80_RS18495 WP_071070424.1 4081616..4083352(+) (pilF) [Cupriavidus malaysiensis strain USMAA1020 isolate pure]
ATGACACTAGGTCTCGCTCTCGCGCAAAGCCGCCGCATCGCTCCGGCCCTTCTCGCCCAGCTCGAACAGGCCGCGCGGGA
AAAGAAGTCGCAACTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCGCGACCTCGCCACCTTCGCCGCCGACA
AGTACCAGCTCCCGCTGCTGGACCTCAGCCAGTTCAACTTCGCCACGGTGCCGCCGGCCCTGGTCGGCAACCGCGAGTTC
CACGCCCACCGGCTGCTGCCGCTGGGCCGGCGCGACAACCGTCTGGTCGTGGCAACCTCCGATCCGTCCAACCAGGCCGG
CCTCGATGCCATCCGCCAGAAACTGAACCTGCCGGTCGACGCGGTCGTGGTCGAGCACGACAAGCTGCTGAAGCACGTGC
GCTCGGCCGGCGAGGAAAGCAGTACGCTGCGCGCCGCCGGCCCGGTGGCGGCCGAGCACAAGCTGATCGAGTACGACCCG
GTCGCCTCGGCGGGAGCCCAGCGCAACAAGGCGCCCAACGACATCGACGACGCACCGGTCGTGCGCTTCCTGCACAAGCT
GCTGACCGAGGCCTTCCACCGGGGCGCATCCGACCTGCACTTCGAGCCGTTCGAGACCTTCTACCGCGTGCGCTTCCGCG
TCGACGGCGTGCTGCTGGAGGTGGCCCGCCCCCCGCTGGACATTCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCG
CGCTTGGACATCTCCGAGAAACGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCGAAGGAAAAGGACAA
GGACGGCAAGGACAGCAAGGAGACGGTCGAGAAGGCCATCGATTTCCGTGTGTCGACGCTGCCGACACTGTTCGGCGAGA
AGATCGTCATGCGGATCCTCGACTCCTCGACCGACAAGCTCGACATCGACCGGCTCGGCTACGAGCCCGCGCAGAAGGCG
CTGCTGCTCGAGACCATCAAGCGCCCCTACGGCATGATCCTGGTCACCGGCCCGACCGGCAGCGGCAAGACGGTCTCGCT
CTACACCTTCCTCAACCTGCTCAACCAGGGCGACATCAATATCTCGACGGCCGAGGATCCCGCCGAAATCCAGCTGCCCG
GCATCAACCAGGTCAACGTCAACGAGAAGGCCGGCCTGACCTTCGCCGCCGCGCTGCGCTCCTTCCTGCGCCAGGACCCG
GACGTCATCATGGTGGGCGAGATCCGCGACCTGGAGACCGCGGACATCGCCATCAAGGCCGCCCAGACCGGCCATCTGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGACCACGCTGACGCGGCTGATGAACATGGGCGTGGCGCCGTTCAACATCG
CCTCCAGCGTGCTGCTGATCACCGCCCAGCGGCTGGCGCGCCGGCTGTGCAACTGCAAGCGGCCGGGCGCCCTGGCGCGC
GACGTGCTGCTTGACGCCGGCTTCCACGAGAGCGAACTAGACGGCAGCTGGCAGCCTTACCACCCGGCCGGCTGCGAGCG
CTGCAGCGGCAGCGGCTACAAGGGGCGTTGCGGCATCTACCAGGTGATGCCCATCTCCGAGGCGATGCAGCAGATCATCC
TGTCTCACGGTACGGCGCTGCAGATCGCCGAGCAGGCGCGCCGCGACGGCGTGCTATCGTTGCGGGAAGCCGGCCTGCTG
AAGGTGAAGCAAGGCGTCACCTCGCTGGAAGAAGTGCTGGCGACGACCAATATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-136)

Type II/IV secretion system protein (T2SSE)

(186-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.853

100

0.516
  pilB Acinetobacter baumannii D1279779

50.685

100

0.512
  pilB Acinetobacter baylyi ADP1

51.434

96.54

0.497
  pilB Legionella pneumophila strain ERS1305867

49.464

96.886

0.479
  pilB Vibrio cholerae strain A1552

46.194

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

46.961

93.945

0.441
  pilB Vibrio campbellii strain DS40M4

45.704

94.637

0.433
  pilF Thermus thermophilus HB27

38.382

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.188

97.405

0.372

Multiple sequence alignment