Detailed information NTG0202548

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BKK80_RS18505
• Genome accession: NZ_CP017754
• Coordinates: 4084650..4085543 (+)
• Length: 297 a.a.
• NCBI ID: WP_071015568.1
• Uniprot ID: -
• Organism: Cupriavidus malaysiensis strain USMAA1020 isolate pure
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4079650..4090543

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKK80_RS18485 (BKK80_18485)	ispB	4080313..4081242 (+)	930	WP_071015561.1	octaprenyl diphosphate synthase	-
BKK80_RS18495 (BKK80_18495)	pilF	4081616..4083352 (+)	1737	WP_071070424.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BKK80_RS18500 (BKK80_18500)	pilC	4083381..4084646 (+)	1266	WP_071015565.1	type II secretion system F family protein	Machinery gene
BKK80_RS18505 (BKK80_18505)	pilD	4084650..4085543 (+)	894	WP_071015568.1	prepilin peptidase	Machinery gene
BKK80_RS18510 (BKK80_18510)	coaE	4085598..4086230 (+)	633	WP_071015570.1	dephospho-CoA kinase	-
BKK80_RS18515 (BKK80_18515)	zapD	4086438..4087196 (+)	759	WP_071016592.1	cell division protein ZapD	-
BKK80_RS18520 (BKK80_18520)	-	4087281..4087472 (+)	192	WP_071015571.1	DNA gyrase inhibitor YacG	-
BKK80_RS18525 (BKK80_18525)	-	4087513..4087941 (-)	429	WP_071015573.1	NUDIX domain-containing protein	-
BKK80_RS18530 (BKK80_18530)	-	4087941..4088831 (-)	891	WP_071015574.1	ATP-binding protein	-
BKK80_RS18535 (BKK80_18535)	argJ	4088868..4090094 (-)	1227	WP_071070426.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 31657.61 Da
• Isoelectric Point: 7.8053

>NTDB_id=202548 BKK80_RS18505 WP_071015568.1 4084650..4085543(+) (pilD) [Cupriavidus malaysiensis strain USMAA1020 isolate pure]
MVSAFSPQGAFTIATLAALPGWFLMLAAGLLGLAVGSFLNVVIHRLPRMMEHDEANYIAELRGEPLPHPSRYNLAVPGSA
CPHCGHTLAPWENIPVLSYLLLRGRCSACHAPIGARYPLVELASGVLSVLAAWHFGPTWQGLAALVLVWTLLALTMIDAD
TQLLPDQLTQPLLWLGLLLNLAGVFASLADAVIGAAAGYLLLWSVYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPA
LVLLSSVVGLLFGLVRIALRRQHRDTPFPFGPFIAGAGLLVLFLGAGALPLALGVPG

Nucleotide

• Length: 894 bp

>NTDB_id=202548 BKK80_RS18505 WP_071015568.1 4084650..4085543(+) (pilD) [Cupriavidus malaysiensis strain USMAA1020 isolate pure]
ATGGTGTCCGCATTCTCGCCGCAAGGCGCCTTCACCATCGCCACGCTGGCCGCCCTGCCCGGCTGGTTCCTGATGCTGGC
GGCCGGCCTGCTGGGCCTGGCGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATGGAGCACGACG
AGGCGAACTACATCGCCGAGCTGCGCGGCGAGCCGCTGCCCCATCCGTCGCGCTACAACCTGGCGGTGCCGGGATCGGCA
TGCCCGCACTGCGGGCACACGCTGGCGCCATGGGAGAACATCCCCGTGCTCAGCTACCTGCTGCTGCGCGGGCGCTGTTC
GGCCTGCCACGCACCGATCGGGGCGCGCTATCCGCTGGTGGAACTCGCCAGCGGGGTGCTGAGCGTGCTGGCCGCCTGGC
ACTTCGGGCCGACCTGGCAGGGCCTGGCGGCGCTGGTCCTGGTGTGGACGCTGCTGGCGCTGACCATGATCGACGCCGAT
ACCCAGCTGCTGCCCGACCAGCTGACCCAGCCGCTGCTGTGGCTCGGCCTGCTGCTCAACCTGGCGGGCGTGTTCGCCTC
GCTGGCCGACGCCGTGATCGGCGCGGCAGCCGGCTACCTGCTGCTGTGGAGCGTCTACTGGCTCTTCAAGCTGGTGCGCG
GCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTGATGGCGGCGCTCGGTGCCTGGTTCGGCTGGCAGGCGCTGCCCGCG
CTGGTGCTGCTGTCCTCCGTGGTCGGCCTGCTGTTCGGCCTCGTCCGCATCGCACTGCGCCGGCAGCATCGCGACACGCC
CTTCCCCTTCGGTCCGTTCATCGCCGGAGCGGGCCTGCTGGTACTGTTCCTGGGGGCCGGCGCGCTGCCGCTCGCGCTGG
GCGTGCCGGGCTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (145-254)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (30-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.307	89.899	0.488
pilD	Vibrio campbellii strain DS40M4	53.933	89.899	0.485
pilD	Acinetobacter baumannii D1279779	46.69	96.633	0.451
pilD	Acinetobacter nosocomialis M2	46.341	96.633	0.448
pilD	Neisseria gonorrhoeae MS11	49.027	86.532	0.424