Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CEK59_RS20045 Genome accession   NZ_CP022478
Coordinates   4231798..4232262 (-) Length   154 a.a.
NCBI ID   WP_127891084.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain LW     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4226798..4237262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEK59_RS20030 (CEK59_19940) nadC 4229310..4230158 (+) 849 WP_058161081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CEK59_RS20040 (CEK59_19950) - 4230338..4231498 (-) 1161 WP_241560868.1 O-antigen ligase family protein -
  CEK59_RS20045 (CEK59_19955) pilA 4231798..4232262 (-) 465 WP_127891084.1 pilin Machinery gene
  CEK59_RS20055 (CEK59_19960) pilB 4232493..4234193 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CEK59_RS20060 (CEK59_19965) pilC 4234197..4235414 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  CEK59_RS20065 (CEK59_19970) pilD 4235415..4236287 (+) 873 WP_034001500.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CEK59_RS20070 (CEK59_19975) coaE 4236284..4236895 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  CEK59_RS20075 (CEK59_19980) yacG 4236892..4237092 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16276.68 Da        Isoelectric Point: 9.4108

>NTDB_id=201117 CEK59_RS20045 WP_127891084.1 4231798..4232262(-) (pilA) [Pseudomonas aeruginosa strain LW]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSATPKNTQYDIGFTE
STLLDGSGKSQIQVTDNKDGTVELVATLGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=201117 CEK59_RS20045 WP_127891084.1 4231798..4232262(-) (pilA) [Pseudomonas aeruginosa strain LW]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCGCGACTCCTAAAAATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACTGCA
AAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.737

98.701

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.484

99.351

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

35.393

100

0.409
  pilA Pseudomonas aeruginosa PAK

45.985

88.961

0.409
  pilA2 Legionella pneumophila strain ERS1305867

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

37.662

100

0.377
  pilA Vibrio cholerae strain A1552

37.662

100

0.377
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.662

100

0.377
  pilA Vibrio parahaemolyticus RIMD 2210633

37.255

99.351

0.37
  pilA2 Legionella pneumophila str. Paris

38

97.403

0.37

Multiple sequence alignment