Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A4W85_RS06695 Genome accession   NZ_CP017570
Coordinates   1311370..1312260 (-) Length   296 a.a.
NCBI ID   WP_251944680.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain TMW 1.578     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1306370..1317260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W85_RS06660 (A4W85_06615) - 1307436..1308446 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  A4W85_RS06665 (A4W85_06620) - 1308570..1308908 (-) 339 WP_011374995.1 hypothetical protein -
  A4W85_RS06670 (A4W85_06625) comGF 1308871..1309383 (-) 513 WP_041820873.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W85_RS06675 (A4W85_06630) comGE 1309358..1309663 (-) 306 WP_011374997.1 hypothetical protein Machinery gene
  A4W85_RS06680 (A4W85_06635) comGD 1309650..1310102 (-) 453 WP_035145016.1 competence type IV pilus minor pilin ComGD Machinery gene
  A4W85_RS06685 (A4W85_06640) comGC 1310074..1310373 (-) 300 WP_076632062.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W85_RS06690 (A4W85_06645) comGB 1310370..1311377 (-) 1008 WP_251944679.1 type II secretion system F family protein Machinery gene
  A4W85_RS06695 (A4W85_06650) comGA 1311370..1312260 (-) 891 WP_251944680.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W85_RS06700 (A4W85_06655) - 1312375..1313106 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W85_RS06705 (A4W85_06660) - 1313197..1313697 (-) 501 WP_011375003.1 VanZ family protein -
  A4W85_RS06710 (A4W85_06665) - 1313794..1314168 (+) 375 WP_011375004.1 hypothetical protein -
  A4W85_RS06720 (A4W85_06675) - 1314548..1315747 (-) 1200 WP_251944682.1 hypothetical protein -
  A4W85_RS06725 (A4W85_06680) - 1315755..1316825 (-) 1071 WP_142501005.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32940.78 Da        Isoelectric Point: 6.4932

>NTDB_id=200464 A4W85_RS06695 WP_251944680.1 1311370..1312260(-) (comGA) [Latilactobacillus sakei strain TMW 1.578]
MSTEEKLTTLLKHCAKQRVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYVVSAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGSDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=200464 A4W85_RS06695 WP_251944680.1 1311370..1312260(-) (comGA) [Latilactobacillus sakei strain TMW 1.578]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAGCAACGTGTTCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGATCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTCAACGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAGATTCGCGATTTAG
AGACAGCCCAATACGTGGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCTGAGCTGCAGTTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTTCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

99.662

100

0.997
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.019

89.527

0.385
  comGA/cglA/cilD Streptococcus pneumoniae D39

42.642

89.527

0.382
  comGA/cglA/cilD Streptococcus pneumoniae R6

42.642

89.527

0.382
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

42.642

89.527

0.382
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

42.642

89.527

0.382
  comGA Lactococcus lactis subsp. cremoris KW2

37.793

100

0.382
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

41.241

92.568

0.382
  comYA Streptococcus gordonii str. Challis substr. CH1

40.959

91.554

0.375
  comYA Streptococcus mutans UA140

38.909

92.905

0.361
  comYA Streptococcus mutans UA159

38.909

92.905

0.361

Multiple sequence alignment