Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   A4W85_RS06670 Genome accession   NZ_CP017570
Coordinates   1308871..1309383 (-) Length   170 a.a.
NCBI ID   WP_041820873.1    Uniprot ID   A0AAX0VCR7
Organism   Latilactobacillus sakei strain TMW 1.578     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1303871..1314383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W85_RS06640 (A4W85_06595) - 1304797..1305162 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  A4W85_RS06650 (A4W85_06605) - 1305705..1306163 (-) 459 WP_096584650.1 laaL -
  A4W85_RS06655 (A4W85_06610) - 1306218..1307414 (-) 1197 WP_011374993.1 acetate/propionate family kinase -
  A4W85_RS06660 (A4W85_06615) - 1307436..1308446 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  A4W85_RS06665 (A4W85_06620) - 1308570..1308908 (-) 339 WP_011374995.1 hypothetical protein -
  A4W85_RS06670 (A4W85_06625) comGF 1308871..1309383 (-) 513 WP_041820873.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W85_RS06675 (A4W85_06630) comGE 1309358..1309663 (-) 306 WP_011374997.1 hypothetical protein Machinery gene
  A4W85_RS06680 (A4W85_06635) comGD 1309650..1310102 (-) 453 WP_035145016.1 competence type IV pilus minor pilin ComGD Machinery gene
  A4W85_RS06685 (A4W85_06640) comGC 1310074..1310373 (-) 300 WP_076632062.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W85_RS06690 (A4W85_06645) comGB 1310370..1311377 (-) 1008 WP_251944679.1 type II secretion system F family protein Machinery gene
  A4W85_RS06695 (A4W85_06650) comGA 1311370..1312260 (-) 891 WP_251944680.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W85_RS06700 (A4W85_06655) - 1312375..1313106 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W85_RS06705 (A4W85_06660) - 1313197..1313697 (-) 501 WP_011375003.1 VanZ family protein -
  A4W85_RS06710 (A4W85_06665) - 1313794..1314168 (+) 375 WP_011375004.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19734.66 Da        Isoelectric Point: 6.9902

>NTDB_id=200459 A4W85_RS06670 WP_041820873.1 1308871..1309383(-) (comGF) [Latilactobacillus sakei strain TMW 1.578]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLDGYMPLLGKLKQLQLDYQRPFVQIDTTFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=200459 A4W85_RS06670 WP_041820873.1 1308871..1309383(-) (comGF) [Latilactobacillus sakei strain TMW 1.578]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTGACACAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCAGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGTCAATTAGATGGGTATATGCCATTATTAGGCAAACTCAAGCAACTTCAACTTGACTATCAGC
GACCGTTTGTTCAAATCGATACGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATCAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

100

100

1

Multiple sequence alignment