Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BHS01_RS07280 Genome accession   NZ_CP017195
Coordinates   1478478..1478876 (-) Length   132 a.a.
NCBI ID   WP_109834250.1    Uniprot ID   -
Organism   Lactococcus paracarnosus strain TMW 2.1615     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1473478..1483876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHS01_RS07250 (BHS01_07260) atpF 1473780..1474289 (-) 510 WP_109834256.1 F0F1 ATP synthase subunit B -
  BHS01_RS07255 (BHS01_07265) atpB 1474302..1475012 (-) 711 WP_109834255.1 F0F1 ATP synthase subunit A -
  BHS01_RS07260 (BHS01_07270) - 1475049..1475249 (-) 201 WP_109834254.1 F0F1 ATP synthase subunit C -
  BHS01_RS07265 (BHS01_07275) - 1475403..1476197 (-) 795 WP_109834253.1 alpha/beta hydrolase -
  BHS01_RS07270 - 1476260..1476559 (-) 300 Protein_1440 ComEC/Rec2 family competence protein -
  BHS01_RS07275 (BHS01_07280) - 1476596..1478314 (-) 1719 WP_223270997.1 ComEC/Rec2 family competence protein -
  BHS01_RS07280 (BHS01_07285) comEA 1478478..1478876 (-) 399 WP_109834250.1 helix-hairpin-helix domain-containing protein Machinery gene
  BHS01_RS07285 - 1478909..1479169 (-) 261 WP_162542400.1 hypothetical protein -
  BHS01_RS07290 (BHS01_07290) - 1479229..1480449 (-) 1221 WP_109834248.1 ABC transporter permease -
  BHS01_RS07295 (BHS01_07295) - 1480442..1481335 (-) 894 WP_109834247.1 ABC transporter ATP-binding protein -
  BHS01_RS07300 (BHS01_07300) - 1481421..1481855 (-) 435 WP_109834246.1 hypothetical protein -
  BHS01_RS07305 (BHS01_07305) - 1481855..1482070 (-) 216 WP_097024480.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 14085.70 Da        Isoelectric Point: 4.2347

>NTDB_id=196576 BHS01_RS07280 WP_109834250.1 1478478..1478876(-) (comEA) [Lactococcus paracarnosus strain TMW 2.1615]
MKQDSRVNDLLLLAGGVTDQADTKSINLAQKLTDEMVVYVASIGEDSPVEIAGTKGDVGADSIETQSTDKININTADLAQ
LQRLSGVGMKKAQDIIDYREQNGKFNQPEDLGNVSGFGEKSLEKLKGSVAVD

Nucleotide


Download         Length: 399 bp        

>NTDB_id=196576 BHS01_RS07280 WP_109834250.1 1478478..1478876(-) (comEA) [Lactococcus paracarnosus strain TMW 2.1615]
TTGAAACAGGATAGTCGGGTTAACGATCTCTTATTATTAGCTGGGGGCGTAACGGATCAGGCAGATACCAAGTCGATTAA
TCTAGCTCAGAAACTGACGGATGAGATGGTCGTCTACGTGGCAAGTATTGGTGAAGATAGTCCAGTTGAGATAGCAGGAA
CTAAAGGTGATGTTGGTGCAGATAGCATAGAAACGCAGTCGACGGATAAAATCAATATTAATACGGCAGATTTAGCTCAG
CTTCAAAGGCTAAGTGGCGTTGGCATGAAAAAGGCACAAGACATTATCGACTATCGGGAACAAAATGGCAAGTTTAATCA
GCCAGAAGACTTAGGAAATGTGTCTGGATTCGGGGAGAAGTCGCTTGAAAAACTTAAAGGGAGTGTGGCAGTGGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(68-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

53.676

100

0.553
  comEA/celA/cilE Streptococcus mitis SK321

52.344

96.97

0.508
  comEA/celA/cilE Streptococcus mitis NCTC 12261

50.781

96.97

0.492
  comEA/celA/cilE Streptococcus pneumoniae D39

50

96.97

0.485
  comEA/celA/cilE Streptococcus pneumoniae Rx1

50

96.97

0.485
  comEA/celA/cilE Streptococcus pneumoniae R6

50

96.97

0.485
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

48.438

96.97

0.47
  comEA Latilactobacillus sakei subsp. sakei 23K

42.759

100

0.47
  comEA Streptococcus thermophilus LMD-9

46.212

100

0.462
  comEA Bacillus subtilis subsp. subtilis str. 168

44.03

100

0.447
  comEA Staphylococcus aureus N315

38.168

99.242

0.379

Multiple sequence alignment