Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BARD7_RS11415 Genome accession   NZ_CP016913
Coordinates   2333190..2333804 (-) Length   204 a.a.
NCBI ID   WP_065981917.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain RD7-7     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2328190..2338804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BARD7_RS11390 (BARD7_02337) rpsT 2328490..2328756 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  BARD7_RS11395 (BARD7_02338) holA 2328774..2329815 (-) 1042 Protein_2254 DNA polymerase III subunit delta -
  BARD7_RS18985 - 2329855..2330009 (-) 155 Protein_2255 hypothetical protein -
  BARD7_RS11400 (BARD7_02339) - 2330050..2330184 (+) 135 WP_003152870.1 YqzM family protein -
  BARD7_RS11405 (BARD7_02340) comEC 2330203..2332554 (-) 2352 WP_065981915.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BARD7_RS11410 (BARD7_02341) - 2332555..2333124 (-) 570 WP_065981916.1 ComE operon protein 2 -
  BARD7_RS11415 (BARD7_02342) comEA 2333190..2333804 (-) 615 WP_065981917.1 helix-hairpin-helix domain-containing protein Machinery gene
  BARD7_RS11420 (BARD7_02343) comER 2333864..2334685 (+) 822 WP_065981918.1 late competence protein ComER -
  BARD7_RS11425 (BARD7_02344) - 2334754..2335491 (-) 738 WP_065981919.1 class I SAM-dependent DNA methyltransferase -
  BARD7_RS11430 (BARD7_02345) rsfS 2335488..2335844 (-) 357 WP_045510454.1 ribosome silencing factor -
  BARD7_RS11435 (BARD7_02346) yqeK 2335861..2336421 (-) 561 WP_032856887.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BARD7_RS11440 (BARD7_02347) - 2336411..2336980 (-) 570 WP_065981920.1 nicotinate-nucleotide adenylyltransferase -
  BARD7_RS11445 (BARD7_02348) yhbY 2336991..2337281 (-) 291 WP_014470707.1 ribosome assembly RNA-binding protein YhbY -
  BARD7_RS11450 (BARD7_02349) aroE 2337275..2338117 (-) 843 WP_014470708.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22165.44 Da        Isoelectric Point: 6.9665

>NTDB_id=193639 BARD7_RS11415 WP_065981917.1 2333190..2333804(-) (comEA) [Bacillus amyloliquefaciens strain RD7-7]
MTKVMMTERQRQYLKILGVCLAAGAVLICILLFRPEKEEPVKQQSVLGQTASAPVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTVVYIPSEGEKENPPKTAAGGKENASININTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=193639 BARD7_RS11415 WP_065981917.1 2333190..2333804(-) (comEA) [Bacillus amyloliquefaciens strain RD7-7]
ATGACGAAAGTGATGATGACGGAGCGGCAGAGGCAGTACCTGAAAATTCTTGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
GATCTGCATTTTGCTGTTTCGTCCCGAAAAAGAAGAGCCTGTCAAACAGCAGTCCGTTCTCGGCCAAACAGCCTCAGCAC
CCGTAACGGACAAAAAAGACACCGCAGACGATAAAATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGAACGGTCGTTTATATCCCGAGTGAAGGCGAAAAGGAGAATCCCCCGAAAACCG
CGGCAGGTGGAAAGGAGAATGCTTCTATCAATATCAATACGGCATCTCTGGATGAACTTCAGGCGATTTCCGGAGTGGGA
CAGAAAAAAGCGGAAGCGATTATTGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.949

95.588

0.554
  comEA Staphylococcus aureus MW2

34.842

100

0.377
  comEA/celA/cilE Streptococcus mitis SK321

35.484

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae R6

35.023

100

0.373
  comEA/celA/cilE Streptococcus pneumoniae D39

35.023

100

0.373
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.023

100

0.373
  comEA/celA/cilE Streptococcus pneumoniae Rx1

35.023

100

0.373
  comEA Staphylococcus aureus N315

33.937

100

0.368
  comEA Lactococcus lactis subsp. cremoris KW2

34.091

100

0.368
  comEA/celA/cilE Streptococcus mitis NCTC 12261

34.562

100

0.368
  comEA Latilactobacillus sakei subsp. sakei 23K

31.624

100

0.363

Multiple sequence alignment