Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   BEN15_RS02255 Genome accession   NZ_CP016877
Coordinates   441275..442216 (-) Length   313 a.a.
NCBI ID   WP_065972616.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain KLDS 3.1003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436275..447216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN15_RS02215 (BEN15_02220) - 436351..437544 (-) 1194 WP_014608740.1 acetate kinase -
  BEN15_RS02220 (BEN15_02225) comYH 437600..438556 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  BEN15_RS02225 (BEN15_02230) comGG 438601..438918 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG -
  BEN15_RS02230 (BEN15_02235) comYF 438896..439333 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  BEN15_RS02235 (BEN15_02240) comGE 439317..439610 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  BEN15_RS02240 (BEN15_02245) comYD 439582..440010 (-) 429 WP_257608452.1 competence type IV pilus minor pilin ComGD Machinery gene
  BEN15_RS02245 (BEN15_02250) comYC 439970..440296 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  BEN15_RS02250 (BEN15_02255) comYB 440293..441393 (-) 1101 WP_142350125.1 competence type IV pilus assembly protein ComGB Machinery gene
  BEN15_RS02255 (BEN15_02260) comGA/cglA/cilD 441275..442216 (-) 942 WP_065972616.1 competence type IV pilus ATPase ComGA Machinery gene
  BEN15_RS02260 (BEN15_02265) - 442296..442658 (-) 363 WP_014622006.1 DUF1033 family protein -
  BEN15_RS02265 (BEN15_02270) rpoC 442816..446454 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35399.53 Da        Isoelectric Point: 6.4142

>NTDB_id=193293 BEN15_RS02255 WP_065972616.1 441275..442216(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain KLDS 3.1003]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIHPIKDALDYRGLYLFAGPVGSGKTTLMHELVQEHFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=193293 BEN15_RS02255 WP_065972616.1 441275..442216(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain KLDS 3.1003]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCATCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCATTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTCTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGTGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.309

99.361

0.639
  comYA Streptococcus gordonii str. Challis substr. CH1

63.259

100

0.633
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.538

99.681

0.613
  comGA Lactococcus lactis subsp. cremoris KW2

51.447

99.361

0.511
  comGA Latilactobacillus sakei subsp. sakei 23K

38.667

95.847

0.371

Multiple sequence alignment