Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   A6L25_RS08440 Genome accession   NZ_CP016652
Coordinates   1642665..1643111 (+) Length   148 a.a.
NCBI ID   WP_002258789.1    Uniprot ID   -
Organism   Neisseria meningitidis strain M08001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1637665..1648111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6L25_RS08420 (A6L25_08420) - 1637871..1638311 (-) 441 WP_101171881.1 hypothetical protein -
  A6L25_RS08425 (A6L25_08425) - 1638913..1639764 (-) 852 WP_002248210.1 sigma-54 factor, Activator interacting domain family protein -
  A6L25_RS08430 (A6L25_08430) katA 1640112..1641626 (+) 1515 WP_002221625.1 catalase KatA -
  A6L25_RS13655 - 1641702..1641839 (+) 138 WP_153312121.1 hypothetical protein -
  A6L25_RS14430 - 1642125..1642253 (-) 129 WP_256864603.1 hypothetical protein -
  A6L25_RS14300 - 1642258..1642407 (+) 150 WP_227718351.1 hypothetical protein -
  A6L25_RS08440 (A6L25_08440) pilE 1642665..1643111 (+) 447 WP_002258789.1 pilin Machinery gene
  A6L25_RS08445 (A6L25_08445) - 1643565..1644065 (+) 501 WP_002220237.1 REP-associated tyrosine transposase -
  A6L25_RS08450 (A6L25_08450) - 1644529..1645095 (+) 567 WP_002236626.1 manganese efflux pump MntP family protein -
  A6L25_RS14435 - 1645223..1645354 (-) 132 WP_257623167.1 hypothetical protein -
  A6L25_RS08455 (A6L25_08455) - 1645371..1647407 (-) 2037 WP_002247939.1 M3 family metallopeptidase -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15590.90 Da        Isoelectric Point: 9.6279

>NTDB_id=190325 A6L25_RS08440 WP_002258789.1 1642665..1643111(+) (pilE) [Neisseria meningitidis strain M08001]
MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEALTLAEGQKSAVVEYYSDNGTFPNSNTSAGIAASNEIK
GKYVASVKVEGNASVASITATMNSSNVNKDIKGKTLVLVGKQNSGSFSWECKKGSVGEKFLPSTCRTK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=190325 A6L25_RS08440 WP_002258789.1 1642665..1643111(+) (pilE) [Neisseria meningitidis strain M08001]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTCATCGCCATCGTCGGTATCTTGGCAGCCGTCGC
CCTGCCCGCATACCAAGACTACACCGCGCGCGCCCAAATGTCCGAAGCCCTGACTTTGGCAGAAGGTCAAAAATCCGCAG
TGGTCGAGTATTATTCCGACAACGGCACATTCCCGAACAGCAATACTTCCGCAGGTATTGCTGCCTCTAACGAGATTAAA
GGTAAGTATGTGGCATCGGTTAAGGTTGAAGGTAATGCCTCTGTTGCTTCTATTACCGCTACCATGAACTCTAGTAATGT
GAATAAGGACATCAAAGGTAAAACCTTGGTACTCGTCGGCAAACAAAACTCCGGCTCGTTCTCTTGGGAATGTAAAAAAG
GTTCTGTGGGCGAAAAATTCTTGCCATCTACCTGCCGCACCAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

58.182

100

0.649
  pilE Neisseria gonorrhoeae strain FA1090

55.556

100

0.608
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.458

100

0.473
  pilA Ralstonia pseudosolanacearum GMI1000

38.012

100

0.439
  pilA/pilA1 Eikenella corrodens VA1

41.026

100

0.432
  comP Acinetobacter baylyi ADP1

41.722

100

0.426
  pilA2 Legionella pneumophila str. Paris

42.657

96.622

0.412
  pilA2 Legionella pneumophila strain ERS1305867

41.259

96.622

0.399
  pilA Pseudomonas aeruginosa PAK

36.601

100

0.378
  pilA Vibrio campbellii strain DS40M4

39.286

94.595

0.372

Multiple sequence alignment