Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   A4W75_RS02860 Genome accession   NZ_CP016467
Coordinates   562659..563030 (+) Length   123 a.a.
NCBI ID   WP_253732800.1    Uniprot ID   -
Organism   Latilactobacillus curvatus strain TMW 1.27     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 557659..568030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W75_RS02830 (A4W75_02735) - 558771..559859 (+) 1089 WP_065825727.1 CAP-associated domain-containing protein -
  A4W75_RS02835 (A4W75_02740) - 559879..560169 (+) 291 WP_004270748.1 YlbG family protein -
  A4W75_RS02840 (A4W75_02745) rsmD 560175..560729 (+) 555 WP_004270750.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  A4W75_RS02845 (A4W75_02750) coaD 560741..561235 (+) 495 WP_065825725.1 pantetheine-phosphate adenylyltransferase -
  A4W75_RS02850 (A4W75_02755) - 561237..562283 (+) 1047 WP_080563410.1 SepM family pheromone-processing serine protease -
  A4W75_RS02855 comEA 562360..562662 (+) 303 WP_253732798.1 hypothetical protein Machinery gene
  A4W75_RS02860 comEA 562659..563030 (+) 372 WP_253732800.1 helix-hairpin-helix domain-containing protein Machinery gene
  A4W75_RS02865 (A4W75_02765) - 563089..563577 (+) 489 WP_111447701.1 ComE operon protein 2 -
  A4W75_RS10990 - 564036..564568 (+) 533 Protein_567 ComEC/Rec2 family competence protein -
  A4W75_RS02875 comEC 564529..565830 (+) 1302 WP_253732803.1 ComEC/Rec2 family competence protein Machinery gene
  A4W75_RS02880 (A4W75_02775) holA 565831..566859 (+) 1029 WP_253732804.1 DNA polymerase III subunit delta -
  A4W75_RS02885 (A4W75_02780) rpsT 566914..567168 (-) 255 WP_004271117.1 30S ribosomal protein S20 -
  A4W75_RS02890 (A4W75_02785) rpsO 567445..567714 (+) 270 WP_004271112.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 123 a.a.        Molecular weight: 13221.77 Da        Isoelectric Point: 4.1849

>NTDB_id=188443 A4W75_RS02860 WP_253732800.1 562659..563030(+) (comEA) [Latilactobacillus curvatus strain TMW 1.27]
MTEAADTSQLNLAQTLSDQLIIYVPTKGEAVQAEELVKAADEPQAATDNQAGASPISTDAKINLNQADATQLQTLNGIGP
KKAEQIIAYRDEQGQFKQIEDLQKVGGIGPKTFEQLRPQICVN

Nucleotide


Download         Length: 372 bp        

>NTDB_id=188443 A4W75_RS02860 WP_253732800.1 562659..563030(+) (comEA) [Latilactobacillus curvatus strain TMW 1.27]
GTGACCGAAGCAGCGGATACGAGTCAGTTGAACTTAGCGCAAACATTGAGTGACCAACTTATTATCTATGTGCCAACTAA
GGGAGAAGCGGTGCAAGCCGAAGAATTAGTGAAAGCGGCAGACGAGCCACAGGCTGCGACTGACAATCAAGCGGGAGCCA
GTCCGATATCAACTGATGCTAAAATCAACCTAAACCAAGCGGATGCGACACAATTGCAAACGCTAAATGGCATCGGTCCT
AAAAAAGCTGAACAAATAATTGCATATCGGGATGAGCAAGGGCAGTTTAAACAAATCGAAGATCTTCAAAAAGTTGGGGG
AATTGGGCCGAAAACGTTTGAACAATTACGGCCACAAATTTGTGTTAATTAG

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(60-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

69.466

100

0.74
  comEA Lactococcus lactis subsp. cremoris KW2

42.188

100

0.439
  comEA Streptococcus thermophilus LMD-9

43.443

99.187

0.431
  comEA Bacillus subtilis subsp. subtilis str. 168

41.732

100

0.431
  comEA/celA/cilE Streptococcus pneumoniae D39

41.27

100

0.423
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.27

100

0.423
  comEA/celA/cilE Streptococcus pneumoniae R6

41.27

100

0.423
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.27

100

0.423
  comEA/celA/cilE Streptococcus mitis SK321

40.8

100

0.415
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

40.476

100

0.415

Multiple sequence alignment