Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BA891_RS12175 Genome accession   NZ_CP016347
Coordinates   2709126..2710349 (+) Length   407 a.a.
NCBI ID   WP_065302866.1    Uniprot ID   -
Organism   Vibrio natriegens strain CCUG 16371     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2704126..2715349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA891_RS12155 (BA891_12155) ampD 2705173..2705718 (-) 546 WP_065302862.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BA891_RS12160 (BA891_12160) nadC 2705811..2706698 (+) 888 WP_065302863.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BA891_RS12165 (BA891_12165) pilA 2706957..2707406 (+) 450 WP_065302864.1 pilin Machinery gene
  BA891_RS12170 (BA891_12170) pilB 2707410..2709095 (+) 1686 WP_065302865.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BA891_RS12175 (BA891_12175) pilC 2709126..2710349 (+) 1224 WP_065302866.1 type II secretion system F family protein Machinery gene
  BA891_RS12180 (BA891_12180) pilD 2710417..2711286 (+) 870 WP_065302867.1 prepilin peptidase Machinery gene
  BA891_RS12185 (BA891_12185) coaE 2711287..2711901 (+) 615 WP_065302868.1 dephospho-CoA kinase -
  BA891_RS12190 (BA891_12190) zapD 2711928..2712668 (+) 741 WP_014232955.1 cell division protein ZapD -
  BA891_RS12195 (BA891_12195) yacG 2712742..2712936 (+) 195 WP_065302869.1 DNA gyrase inhibitor YacG -
  BA891_RS12200 (BA891_12200) rplS 2713240..2713593 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  BA891_RS12205 (BA891_12205) trmD 2713634..2714395 (-) 762 WP_014232957.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  BA891_RS12210 (BA891_12210) rimM 2714423..2714971 (-) 549 WP_014232958.1 ribosome maturation factor RimM -
  BA891_RS12215 (BA891_12215) rpsP 2715002..2715250 (-) 249 WP_005379962.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45067.32 Da        Isoelectric Point: 10.3096

>NTDB_id=187168 BA891_RS12175 WP_065302866.1 2709126..2710349(+) (pilC) [Vibrio natriegens strain CCUG 16371]
MKTTTPQLKNFRWKGINSSGKKTSGQTLAMSEVEVRERLSAQHIKIKKLKKSSISFITKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSNNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDALYTDLIATGELSGNLAEVFERLATYREK
SEELRAKVIKALIYPAMVMLVALGVSYLMLTQVIPEFEKMFSGFGADLPWFTRQVLDLSAWMQNWGSFIALGTLSLIISA
RILVKRSDSFRLMMDRAILKIPVLGAVLSKAAIAKFSRTLATSFSAGIPILTSLKTTSKTSGNLHYQLAVEEVYRDTAAG
MPMYVAMRNCDVFPELVLQMVMIGEESGRLDEMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=187168 BA891_RS12175 WP_065302866.1 2709126..2710349(+) (pilC) [Vibrio natriegens strain CCUG 16371]
ATGAAAACGACGACCCCACAACTAAAGAACTTCCGCTGGAAAGGCATTAATAGCTCAGGAAAAAAAACTTCTGGGCAAAC
GCTGGCCATGAGCGAAGTGGAAGTACGCGAACGACTCAGCGCTCAGCACATAAAAATCAAAAAACTGAAAAAAAGTAGTA
TTTCTTTTATAACCAAACTGAGTCATCGGGTAAAAGGCAAAGACATCACCGTTTTTACTCGTCAAATTTCGACTATGCTG
GTCACTGGTGTCCCACTCGTTCAGGCTTTGAAACTGGTTTCCAATAACCATAAAAAAGCCGAGATGAAGTCTATTTTAAT
GAGCGTAACTCGAGCGGTTGAAGCGGGTACGCCGATGTCAAAAGCAATGCGCACGGCCAGCACACATTTTGACGCACTTT
ATACTGACCTCATCGCAACAGGAGAACTCTCAGGCAATCTGGCAGAAGTCTTTGAACGACTGGCCACTTATCGTGAAAAA
AGTGAGGAACTCAGAGCGAAAGTCATCAAAGCCCTGATCTACCCAGCCATGGTTATGTTGGTTGCGCTCGGCGTGTCCTA
TCTCATGCTCACTCAAGTTATCCCCGAGTTTGAAAAAATGTTTTCTGGGTTTGGTGCAGATTTACCTTGGTTCACCAGAC
AAGTACTGGACTTGTCTGCGTGGATGCAAAATTGGGGTTCATTTATCGCTCTTGGGACACTTAGCCTAATTATATCAGCA
AGAATACTGGTAAAACGTTCTGATTCTTTTCGCTTGATGATGGATCGAGCCATATTGAAAATTCCCGTTCTTGGCGCTGT
GTTGTCCAAGGCTGCAATTGCTAAATTTAGCCGAACCCTGGCCACCAGCTTCAGTGCTGGTATTCCTATTCTGACCAGTT
TAAAAACCACCTCAAAAACATCAGGAAACCTGCACTATCAGCTGGCTGTCGAAGAGGTCTATCGTGATACAGCCGCTGGT
ATGCCAATGTATGTCGCGATGCGTAACTGCGATGTTTTCCCTGAATTAGTGCTACAAATGGTGATGATTGGAGAAGAATC
AGGCAGGCTTGATGAGATGCTCAATAAAGTCGCAACTATTTATGAATTCGAAGTAGATAACACCGTCGATAACTTAAGTA
AGATTCTTGAACCACTGATTATCGTTTTTCTTGGTGTGGTCGTTGGTGGATTAGTAACAGCAATGTACCTGCCAATCTTT
AACTTAATGAGTGTATTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.995

100

0.86
  pilC Vibrio cholerae strain A1552

73.333

99.509

0.73
  pilC Legionella pneumophila strain ERS1305867

41.032

100

0.41
  pilC Acinetobacter baylyi ADP1

41.5

98.28

0.408
  pilC Acinetobacter baumannii D1279779

40.75

98.28

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.648

98.526

0.4
  pilG Neisseria gonorrhoeae MS11

39.801

98.771

0.393
  pilG Neisseria meningitidis 44/76-A

39.552

98.771

0.391

Multiple sequence alignment