Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BA891_RS12170 Genome accession   NZ_CP016347
Coordinates   2707410..2709095 (+) Length   561 a.a.
NCBI ID   WP_065302865.1    Uniprot ID   -
Organism   Vibrio natriegens strain CCUG 16371     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2702410..2714095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA891_RS12150 (BA891_12150) pdhR 2703996..2704763 (-) 768 WP_065302861.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BA891_RS12155 (BA891_12155) ampD 2705173..2705718 (-) 546 WP_065302862.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BA891_RS12160 (BA891_12160) nadC 2705811..2706698 (+) 888 WP_065302863.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BA891_RS12165 (BA891_12165) pilA 2706957..2707406 (+) 450 WP_065302864.1 pilin Machinery gene
  BA891_RS12170 (BA891_12170) pilB 2707410..2709095 (+) 1686 WP_065302865.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BA891_RS12175 (BA891_12175) pilC 2709126..2710349 (+) 1224 WP_065302866.1 type II secretion system F family protein Machinery gene
  BA891_RS12180 (BA891_12180) pilD 2710417..2711286 (+) 870 WP_065302867.1 prepilin peptidase Machinery gene
  BA891_RS12185 (BA891_12185) coaE 2711287..2711901 (+) 615 WP_065302868.1 dephospho-CoA kinase -
  BA891_RS12190 (BA891_12190) zapD 2711928..2712668 (+) 741 WP_014232955.1 cell division protein ZapD -
  BA891_RS12195 (BA891_12195) yacG 2712742..2712936 (+) 195 WP_065302869.1 DNA gyrase inhibitor YacG -
  BA891_RS12200 (BA891_12200) rplS 2713240..2713593 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62046.64 Da        Isoelectric Point: 5.3770

>NTDB_id=187167 BA891_RS12170 WP_065302865.1 2707410..2709095(+) (pilB) [Vibrio natriegens strain CCUG 16371]
MESNLSTILRQSGLISYSQEESLIEQVNASGISMPEALTNSGIFTSSELATHLSSIFGLPCEPLSHYDYTALCQKLGLRE
LITSHHALPLERTPSTLTLAIADPTNQQAEDDFRFATGLQVDLVIVDFSELDAAIRRLYGRALGQERSGLKEINQEELAN
LVDVGADEVDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPNHLSRRLSARIKI
LSKLDIAERRMPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSDQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLTNMGVESYNLASSLSLIIAQRLARKLCPHCKQPQEVTAALQHIGINATDQ
IYQANPNGCNHCTHGYSGRTGIYEVMRFDENLSEALIKGASVHQLEKLAIRNGMSTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=187167 BA891_RS12170 WP_065302865.1 2707410..2709095(+) (pilB) [Vibrio natriegens strain CCUG 16371]
ATGGAGAGCAACCTCTCGACTATTCTTCGCCAATCGGGATTAATTAGTTACTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAACGCTTCCGGTATTTCGATGCCGGAAGCGTTAACTAACTCAGGCATATTCACCTCTAGTGAACTCGCTACTCACCTTA
GTTCAATCTTTGGCTTACCCTGCGAACCCTTATCTCACTATGATTACACCGCCCTGTGCCAAAAATTGGGGCTGCGTGAA
CTGATCACGAGTCACCATGCATTGCCACTGGAGCGAACGCCTTCGACGTTAACTCTCGCCATTGCCGACCCAACGAACCA
ACAAGCTGAAGATGATTTTCGCTTTGCGACAGGATTACAGGTTGATCTTGTCATCGTAGATTTTTCTGAACTTGATGCTG
CGATTCGCCGTTTGTACGGTCGCGCCTTAGGTCAGGAGAGATCAGGCTTAAAAGAGATCAATCAAGAAGAGCTCGCCAAC
CTTGTCGACGTTGGCGCAGATGAAGTCGATAACATCGAAGACTTAAGTCAGGACGAGTCACCGGTTAGCCGCTATATCAA
CCAGATTTTATTGGATGCTGTGCGTAAAGGCGCATCCGATATCCACTTTGAGCCCTATGAAAAAATGTACCGAGTCCGGC
TGCGTTGCGACGGTATTCTGATTGAGACTCAACAGCCGCCAAACCATTTAAGTCGACGCTTATCTGCCCGAATCAAAATT
CTGTCCAAGCTTGATATTGCTGAAAGGCGTATGCCGCAAGACGGTCGAATAAAGTTAAAGCTTAATCAAGACACTGCGAT
TGACATGCGAGTGTCCACCTTACCCACTCTATTCGGAGAAAAAATCGTATTACGACTGCTCGACAGCAGTTCAGCTTCTC
TGGATATCGATAAACTAGGCTACAGTGACCAGCAAAAGCAGCTGTACTTAGATGCTCTGCGTCGTCCACAAGGCATGATT
TTAATGACCGGCCCAACCGGCAGCGGCAAAACCGTCTCGTTGTACACAGGGCTGAGCATACTCAATAAACCAGAGATCAA
TATTTCTACGGCTGAAGACCCGGTCGAAATTAACCTCTCCGGAATTAATCAGGTTCAGGTTCAACCCAAGATAGGCTTTG
GTTTCGCCGAGGCACTGCGCTCTTTCCTGCGTCAAGACCCTGATGTGGTCATGGTGGGAGAAATCCGCGATTTGGACACA
GCAGAAATCGCGATTAAGGCCTCACAAACCGGACACTTAGTCTTGTCGACACTCCACACAAATTCAGCCGCTGAAACCGT
TATCCGCCTAACGAATATGGGCGTTGAAAGCTACAACCTCGCCTCGTCGCTCAGCCTTATTATTGCCCAGCGCTTAGCCC
GTAAACTTTGCCCGCACTGTAAACAACCTCAAGAGGTTACCGCCGCATTACAACATATTGGCATTAACGCAACTGACCAG
ATTTATCAAGCCAACCCAAATGGCTGCAACCACTGTACACACGGCTATTCGGGACGAACTGGCATCTATGAGGTGATGCG
TTTCGATGAAAATCTATCCGAAGCACTGATTAAAGGAGCCTCGGTACATCAACTGGAAAAACTCGCCATCCGTAACGGGA
TGAGCACACTACAAATGTCAGGTATTGAAAAGCTTAAGCAAGGCGTCACAAGCTTTAGCGAACTGCAGCGCGTGCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

89.483

100

0.895
  pilB Vibrio campbellii strain DS40M4

86.096

100

0.861
  pilB Vibrio cholerae strain A1552

72.598

100

0.727
  pilB Acinetobacter baumannii D1279779

50.951

93.761

0.478
  pilB Legionella pneumophila strain ERS1305867

48.799

96.435

0.471
  pilB Acinetobacter baylyi ADP1

50.484

92.157

0.465
  pilF Neisseria gonorrhoeae MS11

44.149

100

0.444
  pilB/pilB1 Synechocystis sp. PCC 6803

35.974

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.429

99.822

0.364
  pilF Thermus thermophilus HB27

36.121

100

0.362

Multiple sequence alignment