Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   A9176_RS06045 Genome accession   NZ_CP016329
Coordinates   1185723..1186133 (+) Length   136 a.a.
NCBI ID   WP_077282594.1    Uniprot ID   -
Organism   Leuconostoc garlicum strain KFRI01     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1163545..1191646 1185723..1186133 within 0


Gene organization within MGE regions


Location: 1163545..1191646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9176_RS05915 (A9176_05885) - 1163545..1163820 (+) 276 WP_010000207.1 HU family DNA-binding protein -
  A9176_RS05920 (A9176_05890) - 1164083..1164310 (+) 228 WP_010000206.1 CsbD family protein -
  A9176_RS05925 (A9176_05895) - 1164512..1165777 (+) 1266 WP_077282558.1 tetratricopeptide repeat protein -
  A9176_RS05930 (A9176_05900) - 1165873..1167078 (+) 1206 WP_077282560.1 CCA tRNA nucleotidyltransferase -
  A9176_RS05935 (A9176_05905) - 1167071..1167571 (+) 501 WP_029509284.1 dihydrofolate reductase -
  A9176_RS05940 (A9176_05910) - 1167579..1168430 (+) 852 WP_077282562.1 DegV family protein -
  A9176_RS05945 (A9176_05915) - 1168573..1169265 (+) 693 WP_242943201.1 SGNH/GDSL hydrolase family protein -
  A9176_RS05950 (A9176_05920) - 1169301..1170332 (-) 1032 WP_077282566.1 lactonase family protein -
  A9176_RS05955 (A9176_05925) - 1170455..1171618 (+) 1164 WP_077282568.1 DNA repair exonuclease -
  A9176_RS05960 (A9176_05930) - 1171624..1173987 (+) 2364 WP_077282570.1 AAA family ATPase -
  A9176_RS05965 (A9176_05935) - 1174003..1174950 (+) 948 WP_077282572.1 HD domain-containing protein -
  A9176_RS05970 (A9176_05940) - 1174984..1175895 (-) 912 WP_029509293.1 peptidyl-prolyl cis-trans isomerase -
  A9176_RS05975 (A9176_05945) - 1175950..1176189 (-) 240 WP_029509294.1 hypothetical protein -
  A9176_RS05980 (A9176_05950) - 1176195..1176626 (-) 432 WP_010000190.1 HIT family protein -
  A9176_RS05985 (A9176_05955) - 1176697..1177440 (+) 744 WP_077282574.1 ABC transporter ATP-binding protein -
  A9176_RS05990 (A9176_05960) - 1177427..1178698 (+) 1272 WP_198150920.1 ABC transporter permease -
  A9176_RS05995 (A9176_05965) trmB 1178746..1179405 (+) 660 WP_029509297.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  A9176_RS06000 (A9176_05970) - 1179860..1180426 (-) 567 WP_077282578.1 hypothetical protein -
  A9176_RS06005 (A9176_05975) - 1180538..1180768 (-) 231 WP_077282580.1 hypothetical protein -
  A9176_RS06010 (A9176_05980) - 1181160..1182203 (-) 1044 WP_077282582.1 site-specific integrase -
  A9176_RS06015 (A9176_05985) - 1182373..1182987 (-) 615 WP_077282584.1 DUF4352 domain-containing protein -
  A9176_RS06020 (A9176_05990) - 1183052..1183561 (-) 510 WP_077282586.1 S24 family peptidase -
  A9176_RS06025 (A9176_05995) - 1183630..1183872 (+) 243 WP_004912700.1 transposase -
  A9176_RS06030 (A9176_06000) - 1183918..1184724 (+) 807 WP_198150921.1 IS3 family transposase -
  A9176_RS06035 (A9176_06005) - 1184773..1185120 (+) 348 WP_077282590.1 hypothetical protein -
  A9176_RS06040 (A9176_06010) - 1185113..1185736 (+) 624 WP_077282592.1 ERF family protein -
  A9176_RS06045 (A9176_06015) ssb 1185723..1186133 (+) 411 WP_077282594.1 single-stranded DNA-binding protein Machinery gene
  A9176_RS06050 (A9176_06020) - 1186145..1186840 (+) 696 WP_077282596.1 putative HNHc nuclease -
  A9176_RS06055 (A9176_06025) - 1186892..1187296 (+) 405 WP_077282599.1 dTDP-glucose pyrophosphorylase -
  A9176_RS08735 - 1187286..1187444 (+) 159 WP_157093699.1 hypothetical protein -
  A9176_RS06060 (A9176_06030) - 1187446..1187727 (+) 282 WP_077282601.1 helix-turn-helix transcriptional regulator -
  A9176_RS06070 (A9176_06040) - 1187918..1188130 (+) 213 WP_077282605.1 hypothetical protein -
  A9176_RS06075 (A9176_06045) - 1188321..1188713 (+) 393 WP_077282607.1 DUF722 domain-containing protein -
  A9176_RS08740 - 1189400..1189570 (+) 171 WP_157093700.1 hypothetical protein -
  A9176_RS06085 (A9176_06055) - 1189619..1189798 (+) 180 WP_077282609.1 hypothetical protein -
  A9176_RS06090 (A9176_06060) - 1189800..1190843 (+) 1044 WP_077282611.1 metallophosphoesterase -
  A9176_RS06095 (A9176_06065) - 1190840..1191646 (-) 807 WP_198150922.1 IS3 family transposase -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 5.1515

>NTDB_id=186978 A9176_RS06045 WP_077282594.1 1185723..1186133(+) (ssb) [Leuconostoc garlicum strain KFRI01]
MNQVNLTGRLTKDIELRYTQSGKAVASGSIAVNRRFKSEGQPDADFINFVMWGKTAENFANYTHKGSQVGLGGEWQTRNY
ENNNGQRVYVNELNATSFDLLDPRGATQTQSNLDSVDPFAAAGKKQIDISDDALPF

Nucleotide


Download         Length: 411 bp        

>NTDB_id=186978 A9176_RS06045 WP_077282594.1 1185723..1186133(+) (ssb) [Leuconostoc garlicum strain KFRI01]
ATGAACCAAGTTAATTTAACAGGACGACTAACAAAAGACATCGAATTACGCTACACACAATCGGGTAAAGCAGTGGCCAG
CGGATCTATTGCGGTTAATCGGCGGTTTAAGTCGGAAGGTCAACCTGATGCAGACTTCATCAACTTCGTGATGTGGGGCA
AGACGGCTGAAAACTTTGCTAACTACACACACAAAGGGTCACAAGTTGGATTAGGTGGCGAGTGGCAGACGCGGAACTAC
GAGAACAACAACGGGCAACGTGTTTACGTGAACGAGCTAAATGCAACAAGTTTTGATTTGCTTGATCCACGAGGCGCAAC
GCAAACACAAAGCAACTTAGATAGCGTTGATCCGTTTGCGGCAGCGGGTAAAAAACAAATCGACATTTCAGACGATGCGC
TGCCCTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

48.235

100

0.603

  ssbA Bacillus subtilis subsp. subtilis str. 168

44.767

100

0.566

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.029

100

0.426

  ssbB/cilA Streptococcus mitis SK321

42.029

100

0.426

  ssbB/cilA Streptococcus mitis NCTC 12261

42.029

100

0.426

  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.647

100

0.426

  ssbB/cilA Streptococcus pneumoniae Rx1

41.304

100

0.419

  ssbB/cilA Streptococcus pneumoniae D39

41.304

100

0.419

  ssbB/cilA Streptococcus pneumoniae R6

41.304

100

0.419

  ssbB Bacillus subtilis subsp. subtilis str. 168

53.846

76.471

0.412

  ssbA Streptococcus mutans UA159

39.706

100

0.397


Multiple sequence alignment