Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC37_RS12735 Genome accession   NZ_CP016321
Coordinates   2603280..2604968 (+) Length   562 a.a.
NCBI ID   WP_088428078.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_037     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2598280..2609968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC37_RS12715 (FORC37_2318) pdhR 2599946..2600713 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FORC37_RS12720 (FORC37_2319) ampD 2601102..2601674 (-) 573 WP_088428076.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC37_RS12725 (FORC37_2320) nadC 2601767..2602654 (+) 888 WP_017421195.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC37_RS12730 (FORC37_2321) pilA 2602877..2603275 (+) 399 WP_088428077.1 pilin Machinery gene
  FORC37_RS12735 (FORC37_2322) pilB 2603280..2604968 (+) 1689 WP_088428078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC37_RS12740 (FORC37_2323) pilC 2605016..2606242 (+) 1227 WP_039536096.1 type II secretion system F family protein Machinery gene
  FORC37_RS12745 (FORC37_2324) pilD 2606336..2607205 (+) 870 WP_088428079.1 prepilin peptidase Machinery gene
  FORC37_RS12750 (FORC37_2325) coaE 2607207..2607815 (+) 609 WP_039536087.1 dephospho-CoA kinase -
  FORC37_RS12755 (FORC37_2326) zapD 2607848..2608588 (+) 741 WP_017421189.1 cell division protein ZapD -
  FORC37_RS12760 (FORC37_2327) yacG 2608651..2608845 (+) 195 WP_039536083.1 DNA gyrase inhibitor YacG -
  FORC37_RS12765 (FORC37_2328) rplS 2609037..2609390 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62359.35 Da        Isoelectric Point: 5.6823

>NTDB_id=186882 FORC37_RS12735 WP_088428078.1 2603280..2604968(+) (pilB) [Vibrio vulnificus strain FORC_037]
MSTNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQDLQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFNEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=186882 FORC37_RS12735 WP_088428078.1 2603280..2604968(+) (pilB) [Vibrio vulnificus strain FORC_037]
ATGTCAACCAACCTTGCCACGATTCTTCGTCAGGCCAATCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTCACTGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCTGATGAACTAACCGAACAGCTAA
GCGCGATTTTTGGCTTACCACGCACAGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGCTTACGTGAG
CTGATCACCCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTAGCCGATTGTCAAGATCTGCAAGCGG
CGATTCGTAGACTTTATGGAAAGAGCATAAAACAGAGCCAAGGCTCTGGGTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATCTAGTCGAGGTTTCAGATGACGAAGTCGAGTCCATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTAT
CAACCAAATCCTGCTTGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTAC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAA
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGCATTAAGCTACGCCTTAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACTTTACCCACGCTGTTTGGTGAAAAAATTGTGCTGCGACTTCTCGACAGCTCGAGTAGCA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGAATG
ATTTTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACGGGCTTGAGTTTGCTCAACCGACCTGAAGT
GAATATCTCCACCGCGGAAGACCCGGTCGAAATTAACCTCCCGGGAATCAACCAAGTACAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCCGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGCCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTAAGTTTAATCATTGCTCAGCGTCTTG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCGCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCCGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTAATGAGCAGCTAGCAGAGGCAATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTCGCTCGGCAAAAAG
GCATGATGACACTCAAAGACTCTGGCCTCGAGAAACTCAAACAAGGCATCACTAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.335

100

0.763
  pilB Vibrio parahaemolyticus RIMD 2210633

75.445

100

0.754
  pilB Vibrio cholerae strain A1552

74.778

100

0.749
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

51.341

92.883

0.477
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.487

100

0.457
  pilF Thermus thermophilus HB27

40.429

99.466

0.402
  pilB/pilB1 Synechocystis sp. PCC 6803

37.063

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment