Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AOT17_RS01555 Genome accession   NZ_CP016298
Coordinates   331357..331791 (+) Length   144 a.a.
NCBI ID   WP_000993717.1    Uniprot ID   A0AAN5WG10
Organism   Acinetobacter baumannii strain CMC-MDR-Ab59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326357..336791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT17_RS01535 (AOT17_01515) - 327749..328636 (+) 888 WP_001017314.1 metal-dependent hydrolase -
  AOT17_RS01540 (AOT17_01520) - 328767..329357 (+) 591 WP_000846931.1 LemA family protein -
  AOT17_RS01545 (AOT17_01525) - 329379..330461 (+) 1083 WP_001246375.1 TPM domain-containing protein -
  AOT17_RS01550 (AOT17_01530) - 330455..331015 (+) 561 WP_000258955.1 TPM domain-containing protein -
  AOT17_RS01555 (AOT17_01535) pilA 331357..331791 (+) 435 WP_000993717.1 pilin Machinery gene
  AOT17_RS01560 (AOT17_01540) - 331849..333159 (+) 1311 WP_000914629.1 O-antigen ligase family protein -
  AOT17_RS01565 (AOT17_01545) - 333267..334895 (+) 1629 WP_001988049.1 PglL family O-oligosaccharyltransferase -
  AOT17_RS01570 (AOT17_01550) bfr 334937..335401 (-) 465 WP_000678123.1 heteropolymeric bacterioferritin subunit Bfr -
  AOT17_RS01575 (AOT17_01555) - 335646..335840 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  AOT17_RS01580 (AOT17_01560) - 336050..336433 (-) 384 WP_000090000.1 RidA family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14873.98 Da        Isoelectric Point: 8.1106

>NTDB_id=186627 AOT17_RS01555 WP_000993717.1 331357..331791(+) (pilA) [Acinetobacter baumannii strain CMC-MDR-Ab59]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQVSTGLADITAGKTNAETKLAEGLTAALTDVTTLGLQQSTNAC
AITANIGTNGASNITCTLKGTSQINGKKIEWIRDADNATNGTTGAWRCKTDVAENLRPKSCGAS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=186627 AOT17_RS01555 WP_000993717.1 331357..331791(+) (pilA) [Acinetobacter baumannii strain CMC-MDR-Ab59]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTTATGATCGTGGTTGCCATTATTGGTATTTTGGCAGCAATTGCGAT
TCCTGCTTATCAAAACTACATTGCTAAGTCACAAGTAAGTACTGGTTTAGCTGATATTACTGCTGGTAAGACAAATGCAG
AAACTAAATTAGCAGAAGGTTTAACTGCGGCATTAACTGATGTAACAACTTTAGGTTTACAACAATCTACGAATGCTTGT
GCTATTACAGCCAATATTGGAACTAATGGTGCAAGTAATATTACTTGTACATTGAAAGGTACATCACAAATTAATGGTAA
AAAAATTGAATGGATCCGTGATGCAGATAATGCTACAAATGGTACGACAGGTGCTTGGCGCTGTAAAACTGATGTAGCTG
AAAACTTACGTCCTAAATCATGTGGTGCTTCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

53.061

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.966

100

0.465
  pilA Vibrio cholerae strain A1552

44.966

100

0.465
  pilA Vibrio cholerae C6706

44.966

100

0.465
  pilA Pseudomonas aeruginosa PAK

41.558

100

0.444
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.366

98.611

0.437
  comP Acinetobacter baylyi ADP1

41.333

100

0.431
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.553

97.917

0.417
  pilA Vibrio campbellii strain DS40M4

40.278

100

0.403
  pilA Vibrio parahaemolyticus RIMD 2210633

44

86.806

0.382

Multiple sequence alignment