Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A6J82_RS00860 Genome accession   NZ_CP020449
Coordinates   144006..144977 (-) Length   323 a.a.
NCBI ID   WP_000250798.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain FDAARGOS_254     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 139006..149977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J82_RS00825 (A6J82_00835) - 139389..139655 (-) 267 Protein_137 hypothetical protein -
  A6J82_RS00830 (A6J82_00840) - 139634..139826 (-) 193 Protein_138 helix-turn-helix transcriptional regulator -
  A6J82_RS00835 (A6J82_00845) - 139954..141147 (-) 1194 WP_000047534.1 acetate kinase -
  A6J82_RS00840 (A6J82_00850) comGH 141179..142153 (-) 975 WP_001008569.1 class I SAM-dependent methyltransferase Machinery gene
  A6J82_RS00845 (A6J82_00855) comGG 142268..142639 (-) 372 WP_000601101.1 competence type IV pilus minor pilin ComGG Machinery gene
  A6J82_RS00850 (A6J82_00860) comGF 142617..143072 (-) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF Machinery gene
  A6J82_RS00855 (A6J82_00865) comGB 143069..144112 (-) 1044 WP_223297804.1 competence type IV pilus assembly protein ComGB Machinery gene
  A6J82_RS00860 (A6J82_00870) comGA 144006..144977 (-) 972 WP_000250798.1 competence type IV pilus ATPase ComGA Machinery gene
  A6J82_RS00865 (A6J82_00880) - 145142..146554 (+) 1413 WP_017771557.1 IS1182 family transposase -
  A6J82_RS00870 (A6J82_00885) ltrA 146604..147881 (-) 1278 WP_001292556.1 group II intron reverse transcriptase/maturase -
  A6J82_RS00875 (A6J82_00890) - 148572..148937 (-) 366 WP_000285374.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36768.25 Da        Isoelectric Point: 6.9167

>NTDB_id=186488 A6J82_RS00860 WP_000250798.1 144006..144977(-) (comGA) [Streptococcus agalactiae strain FDAARGOS_254]
MVQSLAKHVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGARGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSIPGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=186488 A6J82_RS00860 WP_000250798.1 144006..144977(-) (comGA) [Streptococcus agalactiae strain FDAARGOS_254]
ATGGTTCAATCATTAGCAAAGCACGTCATCCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCAAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTGCAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCCCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTCCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAATTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Streptococcus mutans UA159

68.81

96.285

0.663
  comGA Streptococcus mutans UA140

68.81

96.285

0.663
  comGA Streptococcus gordonii str. Challis substr. CH1

63.636

98.762

0.628
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.701

96.285

0.604
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.736

96.285

0.594
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

95.975

0.594
  comGA Lactococcus lactis subsp. cremoris KW2

50.621

99.69

0.505