NTDB

Overview

Name	comGF	Type	Machinery gene
Locus tag	A6J82_RS00850	Genome accession	NZ_CP020449
Coordinates	142617..143072 (-)	Length	151 a.a.
NCBI ID	WP_000793378.1	Uniprot ID	Q8E234
Organism	Streptococcus agalactiae strain FDAARGOS_254
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 137617..148072

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A6J82_RS00815 (A6J82_00825)	proC	137864..138634 (+)	771	WP_001881026.1	pyrroline-5-carboxylate reductase	-
A6J82_RS00820 (A6J82_00830)	-	138655..139320 (-)	666	WP_000008108.1	CPBP family intramembrane glutamic endopeptidase	-
A6J82_RS00825 (A6J82_00835)	-	139389..139655 (-)	267	Protein_137	hypothetical protein	-
A6J82_RS00830 (A6J82_00840)	-	139634..139826 (-)	193	Protein_138	helix-turn-helix transcriptional regulator	-
A6J82_RS00835 (A6J82_00845)	-	139954..141147 (-)	1194	WP_000047534.1	acetate kinase	-
A6J82_RS00840 (A6J82_00850)	comGH	141179..142153 (-)	975	WP_001008569.1	class I SAM-dependent methyltransferase	Machinery gene
A6J82_RS00845 (A6J82_00855)	comGG	142268..142639 (-)	372	WP_000601101.1	competence type IV pilus minor pilin ComGG	Machinery gene
A6J82_RS00850 (A6J82_00860)	comGF	142617..143072 (-)	456	WP_000793378.1	competence type IV pilus minor pilin ComGF	Machinery gene
A6J82_RS00855 (A6J82_00865)	comGB	143069..144112 (-)	1044	WP_223297804.1	competence type IV pilus assembly protein ComGB	Machinery gene
A6J82_RS00860 (A6J82_00870)	comGA	144006..144977 (-)	972	WP_000250798.1	competence type IV pilus ATPase ComGA	Machinery gene
A6J82_RS00865 (A6J82_00880)	-	145142..146554 (+)	1413	WP_017771557.1	IS1182 family transposase	-
A6J82_RS00870 (A6J82_00885)	ltrA	146604..147881 (-)	1278	WP_001292556.1	group II intron reverse transcriptase/maturase	-

Sequence

Protein

Download Length: 151 a.a. Molecular weight: 17509.47 Da Isoelectric Point: 9.7378

                                >NTDB_id=186486 A6J82_RS00850 WP_000793378.1 142617..143072(-) (comGF) [Streptococcus agalactiae strain FDAARGOS_254]

MKNLLLKCKDKKVKAFTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNK

LYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide

Download Length: 456 bp

                                >NTDB_id=186486 A6J82_RS00850 WP_000793378.1 142617..143072(-) (comGF) [Streptococcus agalactiae strain FDAARGOS_254]

ATGAAAAATTTATTGTTAAAATGTAAGGATAAGAAGGTTAAAGCATTTACACTTTTAGAATGTTTGGTAGCATTGGTTAC

AATCACAGGAGCTTTGCTAGTTTATCAAGGACTAACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTC

AGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAGAACAAA

CTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATGATGG

TCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTT

ATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(47-140)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q8E234

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF	Streptococcus mutans UA159	48.571	92.715	0.45
comGF	Streptococcus mutans UA140	48.571	92.715	0.45
comGF/cglF	Streptococcus pneumoniae Rx1	42	99.338	0.417
comGF/cglF	Streptococcus pneumoniae D39	42	99.338	0.417
comGF/cglF	Streptococcus pneumoniae R6	42	99.338	0.417
comGF/cglF	Streptococcus pneumoniae TIGR4	42	99.338	0.417
comGF/cglF	Streptococcus mitis SK321	44.203	91.391	0.404
comGF/cglF	Streptococcus mitis NCTC 12261	43.478	91.391	0.397
comGF	Lactococcus lactis subsp. cremoris KW2	40.58	91.391	0.371

Detailed information NTG0186486