Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   A9404_RS10250 Genome accession   NZ_CP016027
Coordinates   2320260..2320724 (+) Length   154 a.a.
NCBI ID   WP_066103233.1    Uniprot ID   A0A191ZKQ2
Organism   Halothiobacillus diazotrophicus strain LS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2315260..2325724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9404_RS10215 (A9404_10215) - 2315585..2316445 (-) 861 WP_082922904.1 SDR family oxidoreductase -
  A9404_RS10220 (A9404_10220) - 2316623..2317288 (+) 666 WP_066101088.1 Bax inhibitor-1/YccA family protein -
  A9404_RS10225 (A9404_10225) - 2317350..2317535 (+) 186 WP_066101091.1 hypothetical protein -
  A9404_RS13440 - 2317559..2318077 (+) 519 WP_156521307.1 hypothetical protein -
  A9404_RS10240 (A9404_10240) - 2318122..2318946 (+) 825 WP_066101104.1 ProQ/FINO family protein -
  A9404_RS10245 (A9404_10245) - 2319045..2319959 (+) 915 WP_082922905.1 NAD-dependent epimerase/dehydratase family protein -
  A9404_RS10250 (A9404_10250) pilE 2320260..2320724 (+) 465 WP_066103233.1 pilin Machinery gene
  A9404_RS10255 (A9404_10255) - 2320874..2322847 (+) 1974 WP_156521308.1 hypothetical protein -
  A9404_RS10260 (A9404_10260) - 2323096..2324994 (+) 1899 WP_082922906.1 hypothetical protein -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16055.27 Da        Isoelectric Point: 9.4480

>NTDB_id=185430 A9404_RS10250 WP_066103233.1 2320260..2320724(+) (pilE) [Halothiobacillus diazotrophicus strain LS2]
MSKQIQKGFTLIELMIVIAIIGILAAIAIPAYQDYTIRAQVSEGLSLADGAKTAVVEFYNNTGRFPPSNASAGLAKAGSI
TGNYVSQLDVGTAPGQIQVTFSKTSPQRANAAIDKDILTLSASTNGGSVQFRCSPNGVNGTTVPNKYLPSSCRN

Nucleotide


Download         Length: 465 bp        

>NTDB_id=185430 A9404_RS10250 WP_066103233.1 2320260..2320724(+) (pilE) [Halothiobacillus diazotrophicus strain LS2]
ATGAGCAAGCAAATTCAAAAGGGTTTCACCCTGATTGAATTGATGATCGTAATCGCGATCATCGGTATTCTGGCGGCGAT
CGCCATCCCGGCGTACCAGGATTACACCATCCGAGCACAGGTTTCTGAAGGCCTCTCGCTGGCGGATGGCGCCAAGACCG
CCGTAGTTGAGTTTTATAACAACACCGGACGCTTCCCCCCCAGCAATGCTTCGGCAGGATTGGCGAAAGCCGGTTCTATT
ACAGGCAACTACGTTTCTCAGTTGGATGTGGGTACAGCTCCTGGTCAGATACAGGTGACCTTCAGCAAAACTTCGCCACA
GCGTGCAAATGCTGCGATTGACAAAGATATCTTGACTTTGAGCGCGTCGACAAATGGTGGTAGCGTTCAGTTCCGTTGCA
GTCCTAATGGCGTAAACGGCACAACAGTTCCGAACAAATATCTCCCTTCTTCATGCCGTAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-153)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191ZKQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

48.447

100

0.506
  pilA2 Legionella pneumophila strain ERS1305867

48.649

96.104

0.468
  pilE Neisseria gonorrhoeae MS11

44.444

100

0.468
  comP Acinetobacter baylyi ADP1

45.513

100

0.461
  pilA2 Legionella pneumophila str. Paris

47.973

96.104

0.461
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.455
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.829

100

0.435
  pilA/pilA1 Eikenella corrodens VA1

41.615

100

0.435
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.662

100

0.377
  pilA Acinetobacter baumannii strain A118

38.255

96.753

0.37

Multiple sequence alignment